den Hoed M, Hesselink MK, van Kranenburg GP, Westerterp KR. Habitual physical activity in daily life correlates positively with markers for mitochondrial capacity. J Appl Physiol 105: 561-568, 2008. First published May 29, 2008 doi:10.1152/japplphysiol.00091.2008.-Physical exercise training is a powerful tool to maintain or improve mitochondrial density and function (mitochondrial capacity). This study aims to determine whether mitochondrial capacity is also associated with habitual physical activity in daily life (PA DL). The capacity of classic markers for mitochondrial density, i.e., the capacity of citrate synthase (CS) and succinate dehydrogenase (SDH), as well the capacity of cytochrome c oxidase (COX) and -hydroxyacyl-CoA dehydrogenase (HAD), was determined in homogenized muscle biopsy samples obtained from the vastus lateralis muscle of nonexercising healthy young (age 20 Ϯ 2 yr) subjects (31 women, 7 men). PA DL was measured during two periods of 14 days using a triaxial accelerometer for movement registration. CS, SDH, and COX were positively associated with PA DL [P Ͻ 0.05, R ϭ 0.36, 95% confidence interval (CI): 1.3 ⅐ 10 Ϫ4 to 2.2 ⅐ 10 Ϫ3 ; P Ͻ 0.05, R ϭ 0.39, 95% CI: 1.1 ⅐ 10 Ϫ5 to 9.9 ⅐ 10 Ϫ5 ; and P Ͻ 0.05, R ϭ 0.33, 95% CI: 7.5 ⅐ 10 Ϫ6 to 3.6 ⅐ 10 Ϫ4 , respectively], and HAD tended to correlate positively with PA DL (P ϭ 0.06, R ϭ 0.31, 95% CI: Ϫ2.2 ⅐ 10 Ϫ5 to 1.1 ⅐ 10 Ϫ3 ). The population was subsequently stratified based on the intensity of the activities performed. CS was only associated with PADL in subjects spending more time on high-intensity physical activity, whereas HAD was only associated with PADL in subjects spending less time on low intensity physical activity. We are the first to report that even within the range of normal daily life activities, mitochondrial capacity is positively associated with the level of habitual physical activity in daily life. Thus an active lifestyle may help to maintain or improve mitochondrial capacity. accelerometer; intensity; citrate synthase; succinate dehydrogenase; cyctochrome c oxidase; -hydroxyacyl-CoA dehydrogenase IN WESTERNIZED SOCIETIES, the prevalence of Type 2 diabetes and obesity has increased significantly over the past few decades (18,25). A decreased physical activity has been associated with both Type 2 diabetes (13, 17) and obesity (15). Part of the pathophysiology related to these diseases has been attributed to a reduced mitochondrial capacity: the product of mitochondrial density and function (2,16,22).Mitochondria are vital organelles in the oxidative degradation of macronutrients to maintain cellular ATP levels. Mitochondrial aberrations, resulting in a reduced mitochondrial capacity, may therefore seriously impair normal energy and substrate metabolism. Mitochondrial aberrations have not only been reported for metabolic disorders like Type 2 diabetes and obesity but also in diseases like chronic heart failure and chronic obstructive pulmonary disease (11, 24).Proper mitochondrial function and maintenance of mitochondrial capac...