Long-range neuropeptide relay as a central-peripheral communication mechanism for the context-dependent modulation of interval timing behaviors

Zhang, Tianmu; Wu, Zekun; Song, Yutong; Li, Wenjing; Sun, Yanying; Zhang, Xiaoli; Wong, Kyle; Schweizer, Justine; Nguyen, Khoi-Nguyen Ha; Kwan, Alex; Kim, Woo Jae

doi:10.1101/2024.06.03.597273

Cited by 4 publications

(1 citation statement)

References 84 publications

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“…Although the specific population of neurons in the EB relies on the foraging gene for the induction of LMD, there may also be a non-neuronal contribution from the foraging gene to elicit LMD behavior. This is supported by our recent findings that corazonin receptor (CrzR), a gene expressed in glial cells, is a crucial component for LMD 48 . Recent studies have indicated that the foraging gene is expressed across various tissues and is not confined to neuronal populations, extending its reach to glial subtypes and peripheral tissues such as the gastric and reproductive systems 49 .…”

Section: Expression Of Foraging In Pdfr-expressing Specific Cell Popu...supporting

confidence: 60%

Insights into theforagingGene’s Influence on Mating Investments of MaleDrosophila

Li,

Huang,

Kim

2024

Preprint

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Theforaginggene is a key genetic factor that modulates social behavior in insects, primarily by governing the trade-off between individual foraging and group-related activities. It has been associated with various behaviors associated with food search and resource exploitation, thereby playing a crucial role in determining the efficiency of foraging and the overall success of the collective. In this study, we investigate the critical role of theforaginggene in mediating complex interval timing behaviors, particularly mating duration, in the fruit flyDrosophila melanogaster. By examining two distinct variant phenotypes, rover and sitter, we observe specific deficiencies in longer (LMD) and shorter mating duration (SMD) behaviors, respectively, suggesting the gene’s crucial influence on these interval timing mechanisms. Utilizing single-cell RNA sequencing and knockdown experiments, we identify the gene’s significant expression in key neurons involved in learning and memory. However, its impact on mating duration is not observed in these brain regions. Instead, our data reveal the gene’s crucial role in specific neurons expressing Pdfr, a critical regulator of circadian rhythms. Furthermore, the study uncovers sexually dimorphic expression patterns in the brain and highlights the necessity of the gene’s dosage in specific cell populations within the ellipsoid body for normal mating duration. These findings underscore theforaginggene’s pivotal role in mediating complex interval timing behaviors inDrosophila, providing valuable insights into the intricate interplay between genetics, environment, and behavior. This research contributes to a deeper understanding of the genetic and neural mechanisms underlying complex interval timing behaviors, with broader implications for unraveling the function offoraginggene.

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Section: Expression Of Foraging In Pdfr-expressing Specific Cell Popu...supporting

confidence: 60%

Insights into theforagingGene’s Influence on Mating Investments of MaleDrosophila

Li,

Huang,

Kim

2024

Preprint

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A brief history of insect neuropeptide and peptide hormone research

Nässel

2024

Cell Tissue Res

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This review briefly summarizes 50 years of research on insect neuropeptide and peptide hormone (collectively abbreviated NPH) signaling, starting with the sequencing of proctolin in 1975. The first 25 years, before the sequencing of the Drosophila genome, were characterized by efforts to identify novel NPHs by biochemical means, mapping of their distribution in neurons, neurosecretory cells, and endocrine cells of the intestine. Functional studies of NPHs were predominantly dealing with hormonal aspects of peptides and many employed ex vivo assays. With the annotation of the Drosophila genome, and more specifically of the NPHs and their receptors in Drosophila and other insects, a new era followed. This started with matching of NPH ligands to orphan receptors, and studies to localize NPHs with improved detection methods. Important advances were made with introduction of a rich repertoire of innovative molecular genetic approaches to localize and interfere with expression or function of NPHs and their receptors. These methods enabled cell- or circuit-specific interference with NPH signaling for in vivo assays to determine roles in behavior and physiology, imaging of neuronal activity, and analysis of connectivity in peptidergic circuits. Recent years have seen a dramatic increase in reports on the multiple functions of NPHs in development, physiology and behavior. Importantly, we can now appreciate the pleiotropic functions of NPHs, as well as the functional peptidergic “networks” where state dependent NPH signaling ensures behavioral plasticity and systemic homeostasis.

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Male-specific sNPF peptidergic circuits control energy balance for mating duration through neuron-glia interactions

Zhang,

Miao,

Kang

et al. 2024

Preprint

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This study reveals a sexually dimorphic sNPF-sNPF-R circuit in the Drosophila brain that regulates energy balance behavior, specifically the shorter-mating-duration (SMD) response to sexual experience. sNPF is predominantly expressed in neurons, while sNPF-R is expressed in both neurons and glial cells, particularly astrocyte-like glia (ALG) in a subset of cells outside the mushroom body (MB) termed "Rishi cells" (RS cells). Sexual experience induces global alterations in calcium signaling and synaptic plasticity within this circuit, with sNPF-R expressing neurons and glia in RS cells playing a critical role in encoding sexual experience-related information into energy balance behavior related to mating duration. Neuronal glucose metabolism, specifically the Tret1l transporter, is essential for maintaining the high calcium levels and proper function of sNPF neurons near RS cells. This novel and intricate neuron-glia sNPF-sNPF-R network acts as critical circuits within the Drosophila brain for processing interval timing behaviors, highlighting the dynamic interplay between metabolism, neural circuits, and behavior in regulating energy balance.

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Long-range neuropeptide relay as a central-peripheral communication mechanism for the context-dependent modulation of interval timing behaviors

Cited by 4 publications

References 84 publications

Insights into theforagingGene’s Influence on Mating Investments of MaleDrosophila

Insights into theforagingGene’s Influence on Mating Investments of MaleDrosophila

A brief history of insect neuropeptide and peptide hormone research

Male-specific sNPF peptidergic circuits control energy balance for mating duration through neuron-glia interactions

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