Long-Read-Based Genome Sequences of Pandemic and Environmental Vibrio cholerae Strains

Matthey, Noémie; Dörr, Natália C. Drebes; Blokesch, Melanie

doi:10.1128/mra.01574-18

Cited by 24 publications

(38 citation statements)

References 20 publications

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“…Table S2 shows the sequencing details and the features of the closed genomes of the 15 environmental isolates. All genomes showed the dual chromosome architecture that is common for Vibrio species (Okada et al, 2005), a similar overall size of the two chromosomes, and average GC percentages within the same range as the one observed for the pandemic V. cholerae strains N16961 and A1552 (Heidelberg 2000;Matthey et al, 2018).…”

Section: Genome Sequencing Of Environmental V Cholerae Strainssupporting

confidence: 52%

“…This strain is connected to a cholera outbreak in Peru in the 1990s. We recently reported its genome sequence, including more than 1,000 manual gene annotations, according to previous experimental validations (Matthey et al, 2018). Table S2 shows the sequencing details and the features of the closed genomes of the 15 environmental isolates.…”

Section: Genome Sequencing Of Environmental V Cholerae Strainsmentioning

confidence: 99%

“…Furthermore, we were also interested in characterizing the full set of E/I modules in these strains, which would allow us to speculate about the strains' competitive potential against one another. Using the newly assembled genomic data together with the previously reported genome sequence of clade B strain SA5Y (Matthey et al, 2018;Matthey et al, 2019) we determined the E/I modules of these 15 environmental strains and predicted their function based on BlastP analyses ( Fig. 3 and Table S3).…”

Section: Diversity Of T6ss Effectors In Environmental V Cholerae Isomentioning

confidence: 99%

“…T6SS clusters of the environmental strains were identified by searching the Prokka-annotated genomes for conserved genes, such as paar, vgrG and hcp, as well as according to their location when aligned to the genome of the pandemic strain A1552 (Matthey et al, 2018).…”

Section: Characterization Of E/i Modules From Environmental Strainsmentioning

confidence: 99%

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Interbacterial competition and anti-predatory behavior of environmentalVibrio choleraestrains

Doerr

Blokesch²

2020

Preprint

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SummaryVibrio cholerae isolates responsible for cholera pandemics represent only a small portion of the diverse strains belonging to this species. Indeed, most V. cholerae are encountered in aquatic environments. To better understand the emergence of pandemic lineages, it is crucial to discern what differentiates pandemic strains from their environmental relatives. Here, we studied the interaction of environmental V. cholerae with eukaryotic predators or competing bacteria and tested the contributions of the hemolysin and the type VI secretion system (T6SS) to those interactions. Both of these molecular weapons are constitutively active in environmental isolates but subject to tight regulation in the pandemic clade. We showed that several environmental isolates resist amoebal grazing and that this anti-grazing defense relies on the strains’ T6SS and its actin-cross-linking domain (ACD)-containing tip protein. Strains lacking the ACD were unable to defend themselves against grazing amoebae but maintained high levels of T6SS-dependent interbacterial killing. We explored the latter phenotype through whole-genome sequencing of fourteen isolates, which unveiled a wide array of novel T6SS effector and (orphan) immunity proteins. By combining these in silico predictions with experimental validations, we showed that highly similar but nonidentical immunity proteins were insufficient to provide cross-immunity among those wild strains.Originality-Significance StatementThis work contributes to the understanding of phenotypic consequences that differentiate diverse Vibrio cholerae strains. We focused on the type VI secretion system (T6SS) and the pore forming toxin hemolysin, which are tightly regulated in pandemic strains but remain constitutively active in non-pandemic isolates. We unveiled diverse arrays of T6SS effector/immunity modules in a set of environmental strains by long-read whole genome sequencing and de novo assembly. These modules determine whether the strains are able to evade amoebal predation and dictate their level of compatibility or competitiveness with one another.

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Section: Genome Sequencing Of Environmental V Cholerae Strainssupporting

confidence: 52%

Section: Genome Sequencing Of Environmental V Cholerae Strainsmentioning

confidence: 99%

Section: Diversity Of T6ss Effectors In Environmental V Cholerae Isomentioning

confidence: 99%

Section: Characterization Of E/i Modules From Environmental Strainsmentioning

confidence: 99%

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Interbacterial competition and anti-predatory behavior of environmentalVibrio choleraestrains

Doerr

Blokesch²

2020

Preprint

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“…The previous study that addressed TfoY, however, was based on a single strain of V. cholerae, namely strain A1552 (Yildiz and Schoolnik, 1998). This human isolate originated from foodborne transmission on an airplane returning to the US from Peru/South America (Blokesch, 2012a) and belongs to the West-African South American (WASA) lineage of the currently ongoing seventh cholera pandemic (Domman et al, 2017;Matthey et al, 2018). As strain-specific differences are frequently reported, this study was designed to first confirm the generality of TfoX-and TfoY-dependent T6SS activation in pandemic strains of V. cholerae.…”

Section: Resultsmentioning

confidence: 99%

Ecological implications of gene regulation by TfoX and TfoY among diverse Vibrio species

Metzger

Matthey

Stoudmann

et al. 2019

Environmental Microbiology

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Summary Bacteria of the genus Vibrio are common members of aquatic environments where they compete with other prokaryotes and defend themselves against grazing predators. A macromolecular protein complex called the type VI secretion system (T6SS) is used for both purposes. Previous research showed that the sole T6SS of the human pathogen V. cholerae is induced by extracellular (chitin) or intracellular (low c‐di‐GMP levels) cues and that these cues lead to distinctive signalling pathways for which the proteins TfoX and TfoY serve as master regulators. In this study, we tested whether the TfoX‐ and TfoY‐mediated regulation of T6SS, concomitantly with natural competence or motility, was conserved in non‐cholera Vibrio species, and if so, how these regulators affected the production of individual T6SSs in double‐armed vibrios. We show that, alongside representative competence genes, TfoX regulates at least one T6SS in all tested Vibrio species. TfoY, on the other hand, fostered motility in all vibrios but had a more versatile T6SS response in that it did not foster T6SS‐mediated killing in all tested vibrios. Collectively, our data provide evidence that the TfoX‐ and TfoY‐mediated signalling pathways are mostly conserved in diverse Vibrio species and important for signal‐specific T6SS induction.

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Interactions between pili affect the outcome of bacterial competition driven by the type VI secretion system

Otto,

Servajean,

Lemopoulos

et al. 2024

Current Biology

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Long-Read-Based Genome Sequences of Pandemic and Environmental Vibrio cholerae Strains

Cited by 24 publications

References 20 publications

Interbacterial competition and anti-predatory behavior of environmentalVibrio choleraestrains

Interbacterial competition and anti-predatory behavior of environmentalVibrio choleraestrains

Ecological implications of gene regulation by TfoX and TfoY among diverse Vibrio species

Interactions between pili affect the outcome of bacterial competition driven by the type VI secretion system

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