With lipid bilayer−bilayer junctions in mind as model systems for the study of cell−cell junctions, we have
examined adhesion between simple lipid membranes using fluorescence resonance energy transfer (FRET)
and fluorescence interference contrast microscopy (FLIC) to map the interfacial topography. The contact can
take the form of uniform adhesion, with an intermembrane separation of nanometers, or nonuniform adhesion,
in which blisters hundreds of nanometers in height coexist with tight adhesion zones. We find that blisters
can result as a consequence of rapid interbilayer contact induced by osmotic shock. We develop a model for
the formation and stabilization of the blisters, confirming that these phenomena are governed mainly by
hydrodynamic flow in the intermembrane space.