Long‐term adaptation of global transcription and metabolism in the liver of high‐fat diet‐fed C57BL/6J mice

Min, Gyeong; Oh, Hyun-Joo; Kwon, Eun Young; Cho, Yun Young; Shin, Su; Park, Hae Jin; Jeon, Soyeon; Kim, Eun-Jung; Hur, Cheol Goo; Park, Tae Sun; Sung, Mi Kyung; McGregor, Robin A.; Choi, Myung Sook

doi:10.1002/mnfr.201100064

Cited by 76 publications

(98 citation statements)

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“…The observed situation may occur due to short period of dietary intervention. In particular, it has been demonstrated that feeding C57BL/6 J mice with a diet consisting of 20 % fat and 1 % cholesterol induces significant changes in serum glucose, insulin, and HOMA-IR values only after 16 weeks [30]. At the same time, significant diet-induced alteration of serum TC and LDL-C was detected after 6 weeks of dietary intervention [30].…”

Section: Discussionmentioning

confidence: 95%

“…In particular, it has been demonstrated that feeding C57BL/6 J mice with a diet consisting of 20 % fat and 1 % cholesterol induces significant changes in serum glucose, insulin, and HOMA-IR values only after 16 weeks [30]. At the same time, significant diet-induced alteration of serum TC and LDL-C was detected after 6 weeks of dietary intervention [30]. Moreover, feeding of weanling male Sprague-Dawley with a high-fat diet containing 45 % of kilocalorie from fat did not result in a significant change in blood glucose levels after 4, 8, or 12 weeks [31].…”

Section: Discussionmentioning

confidence: 99%

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Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats

Tinkov

Gatiatulina

Popova

et al. 2016

Biol Trace Elem Res

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The primary objective of the current study was to assess the influence of early high-fat feeding on tissue trace element content in young male Wistar rats. Twenty weanling male Wistar rats were divided into two groups fed standard (STD) or high-fat diet (HFD) containing 10 and 31.6 % of total calories from fat, respectively, for 1 month. Serum lipid spectrum, apolipoproteins, glucose, insulin, adiponectin, and leptin levels were assessed. The level of trace elements was estimated using inductively coupled plasma mass spectrometry. High-fat feeding significantly increased epidydimal (EDAT) and retroperitoneal adipose tissue (RPAT), as well as total adipose tissue mass by 34, 103, and 59 %, respectively. Serum leptin levels in HFD animals were twofold higher than those in the control rats. No significant difference in serum lipid spectrum, apolipoproteins, glucose, adiponectin, and insulin was detected between the groups. HFD significantly altered tissue trace element content. In particular, HFD-fed animals were characterized by significantly lower levels of Cu, I, Mn, Se, and Zn in the liver; Cr, V, Co, Cu, Fe, and I content of EDAT; Co, Cu, I, Cr, V, Fe, and Zn concentration in RPAT samples. At the same time, only serum Cu was significantly depressed in HFD-fed animals as compared to the control ones. Hair Co, Mn, Si, and V levels were significantly increased in comparison to the control values, whereas Se and I content was decreased. HFD feeding induced excessive adiposity and altered tissue trace element content in rats without insulin resistance, adiponectin deficiency, and proatherogenic state. Hypothetically, trace element disbalance may precede obesity-associated metabolic disturbances.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats

Tinkov

Gatiatulina

Popova

et al. 2016

Biol Trace Elem Res

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“…Inflammation is a key factor in the progression of fatty liver into more severe stages, and it appears to be required for development of hepatic insulin resistance [33][34][35]. Furthermore, obesity is closely associated with a state of chronic low-grade inflammation [2].…”

Section: Lingonberry and Bilberry Intake Have Antiinflammatory Effectsmentioning

confidence: 99%

Berry intake changes hepatic gene expression and DNA methylation patterns associated with high-fat diet

Heyman-Lindén

Seki

Storm

et al. 2016

The Journal of Nutritional Biochemistry

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The liver is a critical organ for regulation of energy homeostasis and fatty liver disease is closely associated with obesity and insulin resistance. We have previously found that lingonberries, blackcurrants and bilberries prevent, whereas açai berries exacerbate, the development of hepatic steatosis and obesity in the high-fat (HF)-fed C57BL/6J mouse model. In this follow-up study, we investigated the mechanisms behind these effects. Genome-wide hepatic gene expression profiling indicates that the protective effects of lingonberries and bilberries are accounted for by several-fold downregulation of genes involved in acute-phase and inflammatory pathways (e.g. Saa1, Cxcl1, Lcn2). In contrast, açai-fed mice exhibit marked upregulation of genes associated with steatosis (e.g. Cfd, Cidea, Crat) and lipid and cholesterol biosynthesis, which is in line with the exacerbation of HF-induced hepatic steatosis in these mice. In silico transcription factor analysis together with immunoblot analysis identified NF-κB, STAT3 and mTOR as upstream regulators involved in mediating the observed transcriptional effects. To gain further insight into mechanisms involved in the gene expression changes, the HELP-tagging assay was used to identify differentially methylated CpG sites. Compared to the HF control group, lingonberries induced genome-wide hypermethylation and specific hypermethylation of Ncor2, encoding the corepressor NCoR/SMRT implicated in the regulation of pathways of metabolic homeostasis and inflammation. We conclude that the beneficial metabolic effects of lingonberries and bilberries are associated with downregulation of inflammatory pathways, whereas for blackcurrants, exerting similar metabolic effects, different mechanisms of action appear to dominate. NF-κB, STAT3 and mTOR are potential targets of the health-promoting effects of berries.

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“…However, results from anthropometric and biochemical measurements showed that incremental TG was strongly related to an increased risk of NAFLD with the highest ORs, which was consistent with the findings by Fan et al that TG was the most significant predictor (OR: 1.39; 95% CI: 1.25-1.55) in all the blood biochemical indices 9 . The explanation was because, at least in part, excessive accumulation of TG in hepatocytes appeared to be the crucial candidate contributing to hepatic insulin resistance which was dramatically important in the pathogenesis of metabolic syndrome and NAFLD 10,[38][39][40][41] .…”

Section: Discussionmentioning

confidence: 99%

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Long‐term adaptation of global transcription and metabolism in the liver of high‐fat diet‐fed C57BL/6J mice

Cited by 76 publications

References 57 publications

Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats

Early High-Fat Feeding Induces Alteration of Trace Element Content in Tissues of Juvenile Male Wistar Rats

Berry intake changes hepatic gene expression and DNA methylation patterns associated with high-fat diet

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