Long-term administration of magnesium after acoustic trauma caused by gunshot noise in guinea pigs

Abaamrane, Loubna; Raffin, F.; Gál, M.; Avan, Paul; Sendowski, Isabelle

doi:10.1016/j.heares.2008.11.005

Cited by 39 publications

(32 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result suggests that the caspase inhibitor may only accelerate hearing recovery during the immediate post-traumatic period. Faster recovery has been already reported after NIHL for other treatments such as methylprednisolone [7,10]. Cochleograms indicated that the caspase inhibitor significantly preserved hair cell survival.…”

Section: Discussionmentioning

confidence: 53%

“…To limit the individual variability, the methodology consisted of comparing the two ears of the same animal. Indeed, in previous experiments, we did not observe any significant differences in hair cell losses between the left and the right ear in the same animal exposed to impulse noise [7,8]. This methodology has been previously used in different studies [9,10].…”

Section: Discussionmentioning

confidence: 70%

“…The organs of Corti were stained with tetranitro blue tetrazolium and dissected as it was previously described [7]. The missing outer hair cells (OHCs) and inner hair cells (IHCs) were counted in 0.25 mm segments.…”

Section: Histological Analysismentioning

confidence: 99%

See 2 more Smart Citations

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Abaamrane¹,

Raffin²,

Schmerber

et al. 2011

Eur Arch Otorhinolaryngol

View full text Add to dashboard Cite

The therapeutic efficiency of cochlear infusion of two anti-apoptotic substances: a potent calpain inhibitor, leupeptin and a caspase inhibitor, z-VAD-FMK was evaluated in guinea pigs after a gunshot noise-induced trauma (170 dB SPL). A preliminary study showed that hair cell apoptosis appeared within 7 days of the noise trauma. For each animal, one of the cochleae was perfused directly starting 1 h after the trauma with leupeptin or z-VAD-FMK for 7 days via a mini-osmotic pump whereas the other cochlea was untreated. ABR threshold shifts were measured over a 14-day recovery period. The functional hearing study was supplemented by histological analysis. Two days after the trauma significant differences were observed between threshold shifts in the z-VAD-FMK-treated and the non-treated ears. Cochleograms showed that hair cell losses were significantly lower in z-VAD-FMK-treated ears. Regarding the leupeptin treatment, no significant difference between treated and non-treated ears was observed. This work indicates that early direct infusion of z-VAD-FMK into the cochlea accelerates hearing recovery and reduces hair cell loss after gunshot noise-induced trauma. These results suggest that the gunshot noise-induced trauma may involve the caspase pathway rather than the calpain pathway in the apoptotic process.

show abstract

Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 70%

See 1 more Smart Citation

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss

Abaamrane¹,

Raffin²,

Schmerber

et al. 2011

Eur Arch Otorhinolaryngol

View full text Add to dashboard Cite

show abstract

“…For example, glucocortocoids could increase the expression of cochlear anti-oxidant enzymes, which may neutralize the reactive oxygen species resulting from the trauma (Kopke et al, 1999;Wang et al, 2002). Subjects were also provided with magnesium carbonate, as magnesium has been shown to reduce noise-induced cochlear injuries (Abaamrane et al, 2009). Finally, it has been reported that acoustic stimulation following noise trauma can reduce noise-induced hearing loss and abolish the putative neural signs of tinnitus (Fukushima et al, 1990;Niu et al, 2004;Noreña andEggermont, 2005, 2006).…”

Section: Potential Effects Of the Pharmacological Treatments And Acoumentioning

confidence: 99%

Temporary off-frequency listening after noise trauma

et al. 2011

View full text Add to dashboard Cite

“…Conversion of cytocochleograms from position to frequency for correlation with dependent functional losses (e.g. Abaamrane et al, 2009) requires an exact tonotopic frequency map. Our earlier work suggests that the frequency-position relation is compromised by sound trauma in the murine inner ear .…”

Section: Introductionmentioning

confidence: 99%