Long-term and transgenerational effects of cryopreservation on rabbit embryos

Lavara, R.; Baselga, M.; Marco‐Jiménez, Francisco; Vicente, J.S.

doi:10.1016/j.theriogenology.2014.01.030

Cited by 20 publications

(22 citation statements)

References 29 publications

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“…Some authors postulate that these procedures do not induce major anomalies but can lead to morphologic and behavioral features in adult mice derived from frozen embryos [16]. However, Auroux et al [17] found a beneficial effect in longevity in adults, and Lavara et al [22] reported a positive long-term effect on reproduction traits in rederived rabbit females from vitrified embryos, probably due to changes in epigenetic control regions related with reproductive traits.…”

Section: Discussionmentioning

confidence: 99%

“…However, Auroux et al [17] found a beneficial effect in longevity in adults, and Lavara et al [22] reported a positive long-term effect on reproduction traits in rederived rabbit females from vitrified embryos, probably due to changes in epigenetic control regions related with reproductive traits. Values in the same row with different superscript are significantly different (P < 0.05).…”

Section: R Lavara Et Al / Theriomentioning

confidence: 99%

“…Recently, positive long-term effects of cryopreservation and transfer procedures on female reproduction have been reported in rabbits by Lavara et al [22], with changes in epigenetic control regions caused by embryo manipulation or favorable maternal effects the most plausible explana-tions. However, little is known about the possible effect of embryo cryopreservation and transfer on postnatal growth development in mammals.…”

Section: Introductionmentioning

confidence: 99%

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Embryo vitrification in rabbits: Consequences for progeny growth

et al. 2015

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The objective of this research is to examine if there are any effects of the rederivation procedures on rabbit growth pattern and on weight of different organ in adults. For this purpose, three experiments were conducted on two different groups of animals (control group and vitrified-transferred group) to evaluate the possible effect of embryo manipulation (vitrification and transfer procedures) on future growth traits. The first experiment studies body weight from 1 to 9 weeks of age from the two groups. The second experiment describes the growth curve of progeny from experimental groups and analyzes their Gompertz curve parameters, including the estimation of adult body weight. The third experiment has been developed to study if there are any differences in different organ weight in adult males from the two experimental groups. In general, the results indicate that rederivation procedures had effect on the phenotypic expression of growth traits. The results showed that rabbit produced by vitrification and embryo transfer had higher body weight in the first four weeks of age than control progeny. Results from body weight (a parameter) and b parameter estimated by fitting the Gompertz growth curve did not show any difference between experimental groups. However, differences related with growth velocity (k parameter of the Gompertz curve) were observed among them, showing that the control group had higher growth velocity than the vitrified-transferred group. In addition, we found that liver weight at 40th week of age exhibits significant differences between the experimental groups. The liver weight was higher in the control males than in the VF males. Although the present results indicate that vitrification and transfer pro-cedures might affect some traits related with growth in rabbits, further research is needed to assess the mechanisms involved in the appearance of these phenotypes and if these phenotypes could be transferred to the future progeny.

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Section: Discussionmentioning

confidence: 99%

Section: R Lavara Et Al / Theriomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Embryo vitrification in rabbits: Consequences for progeny growth

et al. 2015

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“…Vitrification also affects foetal survival and growth and modifies the placental development at term [60,76] and growth from birth to adult age [77]. Nonetheless, positive long-term effects of cryopreservation and transfer procedures on female offspring reproduction have been reported, namely in the litter size and the number of newborns alive at birth [78].…”

Section: Embryo Cryopreservationmentioning

confidence: 99%

“…A good understanding of the chronology of events that follows the ovulation is crucial to the recovery of preimplantation embryos [78]. At 24 hpc, all the embryos can be found in the isthmus [95].…”

Section: Embryo Recovery and Transfermentioning

confidence: 99%

Embryo Manipulation Techniques in the Rabbit

García¹

2018

New Insights Into Theriogenology

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Rabbits are both productive and classic laboratory animals. Some particularities of female reproductive physiology make the rabbit an extraordinary model for the study of embryology and assisted reproductive techniques. For instance, as the ovulation is induced, the embryo development can be known with accuracy. Embryos are surrounded by a mucin coat which is crucial to prevent embryo mortality. Besides, the anatomy of the uterus does not allow embryo transmigration between both uterine horns, and so it is possible to test different reproductive techniques. Knowledge on early embryo development, and on influencing factors, has allowed to develop new insights into embryo manipulation, such as recovery, transfer, cryopreservation, in vitro fertilisation, cloning, or transgenesis. Also the rabbit may be used as a model for human reproductive health, because rabbit embryo and feto-placental development are similar to the human. This chapter reviews the aspects of the reproductive physiology in the female rabbit and discusses some embryo manipulation techniques available in the species.

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