Long-term biopersistence of tangled oxidized carbon nanotubes inside and outside macrophages in rat subcutaneous tissue

Sato, Yoshinori; Yokoyama, Atsuro; Nodasaka, Yoshinobu; Kohgo, Takao; Motomiya, Kenichi; Matsumoto, Hiroaki; Nakazawa, Eiko; Numata, Tomoko; Zhang, Minfang; Yudasaka, Masako; Hara, Hideyuki; Araki, Rikita; Tsukamoto, Osamu; Saito, Hiroaki; Kamino, Takeo; Watari, Fumio; Tohji, Kazuyuki

doi:10.1038/srep02516

Cited by 45 publications

(46 citation statements)

References 60 publications

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“…Furthermore, previous histological findings indicated that GO was frequently engulfed by macrophage-like cells, and there was evidence of residual graphene in cell lysosomes following phagocytosis 50) . In contrast, long-term (2 year) histological examination revealed that oxidized CNTs had degraded inside macrophages in rat subcutaneous tissue 51) . The degradation of GO-based nanomaterials, and a systematic method for testing the safety of nanocarbon materials, must be established in the future.…”

Section: Discussionmentioning

confidence: 99%

Graphene oxide coating facilitates the bioactivity of scaffold material for tissue engineering

Nishida

Miyaji

Takita

et al. 2014

Jpn. J. Appl. Phys.

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Carbon-based nanomaterials are being investigated for biomedical applications. Graphene oxide (GO), a monolayer of carbon, holds promise as a tissue engineering substrate due to its unique physicochemical properties. The aim of this study was to evaluate the effect of a GO coating on cell proliferation and differentiation in vitro. We also assessed the bioactivities of collagen scaffolds coated with different concentrations of GO in rats. The results showed that GO affects both cell proliferation and differentiation, and improves the properties of collagen scaffolds. Subcutaneous implant tests showed that low concentrations of GO scaffold enhances cell in-growth and is highly biodegradable, whereas high concentrations of GO coating resulted in adverse biological effects.Consequently, scaffolds modified with a suitable concentration of GO are useful as a bioactive material for tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Graphene oxide coating facilitates the bioactivity of scaffold material for tissue engineering

Nishida

Miyaji

Takita

et al. 2014

Jpn. J. Appl. Phys.

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“…In a following work, treating MWCNTs by sonication in H 2 O caused the implementation of O-containing functionalities (OH, C-O-C and COOH) and no considerable harm to the basic CNTs structure (Vanyorek et al 2014;Parodi et al 2014). The production of functional groups is reflected in the withdrawal of -CH n groups existing on the pristine CNTs and the presence of H bonding between the CNTs and the aqueous medium (Wang et al 2014a, b, c;Sato et al 2013). Previously, soluble and oxidized SWCNTs arranged by supramolecular attachment of functionalized organic crown ethers (2-aminomethyl-18-crown-6).…”

Section: Oxidized Functionalization Cntsmentioning

confidence: 96%

Multifunctionalized Carbon Nanotubes Polymer Composites: Properties and Applications

Julkapli

Sapuan

2015

Advanced Structured Materials

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Carbon nanotubes (CNTs) is a rigid rod-like nanoscale material produced from carbon in powder, liquid, or gel form via acid or chemical hydrolysis. Due to its unique and exceptional renewability, biodegradability, mechanical, physicochemical properties, and abundance, the incorporation associated with a small quantity of CNTs to polymeric matrices enhance the mechanical and thermal resistance, and also stability of the latter by several orders of magnitude. Moreover, NCC-derived carbon materials are of no serious threat to the environment, providing further impetus for the development and applications of this green and renewable biomaterial for lightweight and degradable composites. Surface functionalization of CNTs remains the focus of CNTs research in tailoring its properties for dispersion in hydrophilic and hydrophobic media. Through functionalization, the attachment of appropriate chemical functionalities between conjugated sp 2 of CNTs and polymeric matrix is established. It is thus of utmost importance that the tools and protocols for imaging CNTs in a complex matrix and quantify its reinforcement, antimicrobial, stability, hydrophilicity, and biodegradability are be developed.

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“…32 A number of studies have investigated the biodegradability of CNTs in an attempt to address this issue. [37][38][39] However, CNTs are inherently stable, and degradation by chemical modification has yet to be developed. Therefore, the present study examined the optimal CNT shape and size that can maximize biocompatibility using two different types of CNT.…”

Section: Discussionmentioning

confidence: 99%

Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells

Haniu¹,

Matsuda²,

Tsukahara³

et al. 2014

IJN

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This study aimed to investigate the influence of the shape and size of multi-walled carbon nanotubes (MWCNTs) and cup-stacked carbon nanotubes (CSCNTs) on biological responses in vitro. Three types of MWCNTs – VGCF ® -X, VGCF ® -S, and VGCF ® (vapor grown carbon fibers; with diameters of 15, 80, and 150 nm, respectively) – and three CSCNTs of different lengths (CS-L, 20–80 μm; CS-S, 0.5–20 μm; and CS-M, of intermediate length) were tested. Human bronchial epithelial (BEAS-2B) and malignant pleural mesothelioma cells were exposed to the CNTs (1–50 μg/mL), and cell viability, permeability, uptake, total reactive oxygen species/superoxide production, and intracellular acidity were measured. CSCNTs were less toxic than MWCNTs in both cell types over a 24-hour exposure period. The cytotoxicity of endocytosed MWCNTs varied according to cell type/size, while that of CSCNTs depended on tube length irrespective of cell type. CNT diameter and length influenced cell aggregation and injury extent. Intracellular acidity increased independently of lysosomal activity along with the number of vacuoles in BEAS-2B cells exposed for 24 hours to either CNT (concentration, 10 μg/mL). However, total reactive oxygen species/superoxide generation did not contribute to cytotoxicity. The results demonstrate that CSCNTs could be suitable for biological applications and that CNT shape and size can have differential effects depending on cell type, which can be exploited in the development of highly specialized, biocompatible CNTs.

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Long-term biopersistence of tangled oxidized carbon nanotubes inside and outside macrophages in rat subcutaneous tissue

Cited by 45 publications

References 60 publications

Graphene oxide coating facilitates the bioactivity of scaffold material for tissue engineering

Graphene oxide coating facilitates the bioactivity of scaffold material for tissue engineering

Multifunctionalized Carbon Nanotubes Polymer Composites: Properties and Applications

Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells

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