Long-term changes in the properties of skin-derived fibroblasts following irradiation of the head and neck

Gehrke, Thomas; Scherzad, Agmal; Hackenberg, Stephan; Ickrath, Pascal; Schendzielorz, Philipp; Hagen, Rudolf; Kleinsasser, Norbert

doi:10.3892/ol.2017.6593

Cited by 2 publications

(2 citation statements)

References 41 publications

(33 reference statements)

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“…In the irradiated pouch (day 28), neutrophilic and lymphocytic infiltration was observed in the submucosa (densely populated area with nuclei) and destruction of the epithelium was still evident. Plump fibroblasts were seen throughout the sample as commonly seen in human patients that have been exposed to radiotherapy 13 . The formation of dense fibrosis also was observed (blue arrow).…”

Section: Resultsmentioning

confidence: 58%

Radiation‐induced oral mucositis hamster model using a linear accelerator enhances clinical relevance of preclinical studies for treatment strategy investigation

Jordan

Bradford

Cheek

et al. 2021

Anim Models and Exp Med

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Translational animal models for oral mucositis (OM) are necessary to simulate and assess the bioclinical effects and response in humans. These models should simulate high levels of radiation exposure that leads to oxidative stress and inflammatory‐initiated tissue changes. Hamster models have been extensively studied to observe pathological effects of radiation exposure and help in the development of effective treatments. To successfully evaluate the potential for treatment regimens with consistency and relevance, a radiation‐induced OM hamster model was developed using a clinical linear accelerator utilized by cancer patients daily. The dose exposure to the isolated, everted cheek pouch of a hamster, as well as the progression of injury, pro‐inflammatory marker, histological, and elasticity analyses of the buccal pouch were conducted to verify replicability and reproducibility of the injury model. The findings from this model demonstrated its ability to consistently induce injury and resolution over 28 days using an acute dose of 60 Gy. This model was developed to enhance clinical relevance when evaluating potential efficacious treatments and can now be utilized in efficacy studies to better evaluate developed therapeutics in a preclinical model that is easy to translate to clinical studies..

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Section: Resultsmentioning

confidence: 58%

Radiation‐induced oral mucositis hamster model using a linear accelerator enhances clinical relevance of preclinical studies for treatment strategy investigation

Jordan

Bradford

Cheek

et al. 2021

Anim Models and Exp Med

View full text Add to dashboard Cite

show abstract

“…Human fibroblasts are the predominant stromal cell type of the connective tissue [ 70 ], and the main effector of many physiological and pathological processes, such as ECM turnover and homeostasis [ 71 ], epidermal regeneration [ 72 ], wound healing and tumor [ 73 , 74 ]. The dermis of normal skin consists of two different fibroblast subpopulations with distinct cell morphology, gene expression patterns, and capability [ 75 , 76 ].…”

Section: Dermal Fibroblasts In Normal Skin: Features and Functions Before Melanoma Occurrencementioning

confidence: 99%

Influence of Tumor Microenvironment and Fibroblast Population Plasticity on Melanoma Growth, Therapy Resistance and Immunoescape

Romano¹,

Belviso²,

Venuta³

et al. 2021

IJMS

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Cutaneous melanoma (CM) tissue represents a network constituted by cancer cells and tumor microenvironment (TME). A key feature of CM is the high structural and cellular plasticity of TME, allowing its evolution with disease and adaptation to cancer cell and environmental alterations. In particular, during melanoma development and progression each component of TME by interacting with each other and with cancer cells is subjected to dramatic structural and cellular modifications. These alterations affect extracellular matrix (ECM) remodelling, phenotypic profile of stromal cells, cancer growth and therapeutic response. The stromal fibroblast populations of the TME include normal fibroblasts and melanoma-associated fibroblasts (MAFs) that are highly abundant and flexible cell types interacting with melanoma and stromal cells and differently influencing CM outcomes. The shift from the normal microenvironment to TME and from normal fibroblasts to MAFs deeply sustains CM growth. Hence, in this article we review the features of the normal microenvironment and TME and describe the phenotypic plasticity of normal dermal fibroblasts and MAFs, highlighting their roles in normal skin homeostasis and TME regulation. Moreover, we discuss the influence of MAFs and their secretory profiles on TME remodelling, melanoma progression, targeted therapy resistance and immunosurveillance, highlighting the cellular interactions, the signalling pathways and molecules involved in these processes.

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Long-term changes in the properties of skin-derived fibroblasts following irradiation of the head and neck

Cited by 2 publications

References 41 publications

Radiation‐induced oral mucositis hamster model using a linear accelerator enhances clinical relevance of preclinical studies for treatment strategy investigation

Radiation‐induced oral mucositis hamster model using a linear accelerator enhances clinical relevance of preclinical studies for treatment strategy investigation

Influence of Tumor Microenvironment and Fibroblast Population Plasticity on Melanoma Growth, Therapy Resistance and Immunoescape

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