Long-term culture and characterization of goat primordial germ cells

Kühholzer, B.; Baguisi, A.; Overström, E.W.

doi:10.1016/s0093-691x(00)00253-3

Cited by 30 publications

(19 citation statements)

References 13 publications

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“…Anti-SSEA-1 antibody also reacts specifically with mouse embryonic carcinoma (EC) cells, mouse embryonic germ (EG) cells, and human EG cells. Anti-SSEA-1 antibody has also been used for the detection of PGCs in several species [19][20][21][22], but human EC cells do not react with anti-SSEA-1 antibody.…”

Section: Discussionmentioning

confidence: 99%

Development of Novel Markers for the Characterization of Chicken Primordial Germ Cells

et al. 2005

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This study was undertaken to develop novel markers for chicken primordial germ cells (PGCs), which are of potentially enormous value in transgenic research. Gonadal cells collected from 5.5-day-old chicken embryos were cultured in a Dulbecco's minimal essential medium and the PGC colonies formed during the primary culture period were subcultured three times. Characterization of the PGCs with the candidate marker reagents was performed on the mixed cell population 2 hours after seeding, after the primary culture period (day 10), and after the third passage (day 40). Mouse embryonic stem (

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Section: Discussionmentioning

confidence: 99%

Development of Novel Markers for the Characterization of Chicken Primordial Germ Cells

et al. 2005

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“…Caprine ES or ES-like cell lines have been reported as well [43]. Also, a goat EG cell line was reported by [44]. Ovine ES or ES-like cell lines have also been reported [22].…”

Section: Review Of Ungulate Es Cell Line Literaturementioning

confidence: 99%

The Pursuit of ES Cell Lines of Domesticated Ungulates

Talbot¹,

Blomberg²

2008

Stem Cell Rev

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In contrast to differentiated cells, embryonic stem cells (ESC) maintain an undifferentiated state, have the ability to self-renew, and exhibit pluripotency, i.e., they can give rise to most if not all somatic cell types and to the germ cells, egg and sperm. These characteristics make ES cell lines important resources for the advancement of human regenerative medicine, and, if established for domesticated ungulates, would help make possible the improvement of farm animals through their contribution to genetic engineering technology. Combining other genetic engineering technologies, such as somatic cell nuclear transfer with ESC technology may result in synergistic gains in the ability to precisely make and study genetic alterations in mammals. Unfortunately, despite significant advances in our understanding of human and mouse ESC, the derivation of ES cell lines from ungulate species has been unsuccessful. This may result from a lack of understanding of species-specific mechanisms that promote or influence cell pluripotency. Thorough molecular characterizations, including the elucidation of stem cell "marker" signaling cascade hierarchy, species-appropriate pluripotency markers, and pluripotency-associated chromatin alterations in the genomes of ungulate species, should improve the chances of developing efficient, reproducible technologies for the establishment of ES cell lines of economically important species like the pig, cow, goat, sheep and horse.

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“…PGCs have strong alkaline phosphatase (AKP) activity in mouse (Hua and Martin, 2001), Naemorhedus goral (Kühholzer et al, 2000), and human (Gould In later-stage oocytes, ma-vas RNA was concentrated at the cortical region (black arrows in B). Obvious positive signals were exhibited somewhere in the peripheral layer of late ovary (black arrow in C).…”

Section: Spermatogonia and Alkaline Phosphatasementioning

confidence: 99%

Cloning and Characterization of a Rice Field Eel vasa-Like Gene cDNA and Its Expression in Gonads During Natural Sex Transformation

Ding

Song

et al. 2007

Biochem Genet

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The vasa (vas)-related gene encodes an RNA helicase protein member of the DEAD-box family and plays key roles in germ-cell formation in higher metazoans. Using degenerate PCR and RACE, we cloned the vasa gene of the rice field eel (Monopterus albus), which is homologous to the Drosophila vasa gene. We named it ma-vas (Monopterus albus vas). Ma-vas encodes a protein of 618 amino acids, which contains all of the known characteristics of vasa homologs. RT-PCR analysis revealed that ma-vas was exclusively expressed in the gonads of the female, intersex, and male. During gonadal natural sex reversal, ma-vas is expressed in oocytes at all stages of oogenesis, in degenerating oocytes of ovotestis, and in spermatogonia and spermatocytes at early stages. The vasa positive signal was also observed in the peripheral layer of late ovary. It was not found, however, in that layer of the testis. Alkaline phosphatase (AKP) staining on the ovary and testis also indicated that some cells had differentiational potential in the peripheral layer of the ovary, suggesting that spermatogonia might arise from cells with AKP and vasa-positive staining in the peripheral layer of the female gonad.

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Long-term culture and characterization of goat primordial germ cells

Cited by 30 publications

References 13 publications

Development of Novel Markers for the Characterization of Chicken Primordial Germ Cells

Development of Novel Markers for the Characterization of Chicken Primordial Germ Cells

The Pursuit of ES Cell Lines of Domesticated Ungulates

Cloning and Characterization of a Rice Field Eel vasa-Like Gene cDNA and Its Expression in Gonads During Natural Sex Transformation

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