Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney

Abstract

In vitro cultures of native fish cell lines are of great importance, both for basic research and applied science. In particular, there is strong demand for long-term growable cell lines from breeding fish, like sturgeon. Here, we describe the culture of cells from Siberian sturgeon (Acipenser baerii) head kidney. The cells have so far been cultured over a period of 12 months (24 passages). Cytochemical and immunocytochemical examination suggests that, in vitro, the cells exhibit markers that are indicative for different cell types. In particular, fat storing cells (adipocytes) were observed, and the expression of cytokeratins and glial fibrilar acidic protein (GFAP) can be concluded on the basis of immuncytochemical analysis. The observation of different morphologies additionally underlines the heterogeneity of the cell population and matches the typical behaviour of in vitro cultures of stem/progenitor cells. Different applications can be imagined.

Isolation of cells from Atlantic sturgeon Acipenser oxyrinchus oxyrinchus and optimization of culture conditions

“…However, the most substantial factor for cell growth is the cell itself. Cells change their function, morphology and proliferation rate during culturing (Ciba et al 2008, Neuhuber et al 2008. These changes require an alteration of culture conditions.…”

“…Recently, the Fraunhofer Research Institution for Marine Biotechnology has developed methods for culturing cells from larval fish and also from tissues (e.g. head, kidney, pancreas, brain, muscle, skin, heart and pyloric caeca) of adult fish from 11 mainly commercially important fish species (Ciba et al 2008, Grunow et al 2010, Langner et al 2011.…”

“…basal culture media supplemented with mammalian sera, such as fetal calf serum (FCS). Several researchers have worked on cell and tissue cultures derived from fish to estimate the optimal growth conditions (Bradford et al 1994, Ghosh & Collodi 1994, Ciba et al 2008.…”

A Novel Xenogeneic Co-Culture System to Examine Neuronal Differentiation Capability of Various Adult Human Stem Cells

“…Such a xenogeneic approach is not uncommon as Anwar et al [10] already described the differentiation of human neural SCs into dopaminergic neurons by co-culture with rat brain slices. Many growth factors are evolutionarily highly conserved [35], [36] and the cross-reactivity of growth factors between different species plays a decisive role while using fetal bovine serum (FBS) in the routine cultivation of any cell type [37], [38], [39]. Furthermore, an analysis of the amino acid sequences of potentially important growth factors via BLAST (http://blast.ncbi.nlm.nih.gov) reveals partial or high homologies between human and rat, so that one can principally assume a sufficient interaction of the rat brain-derived growth factors with receptors on the human SCs as well as with the antibodies on the human growth factor array.…”

New cell line from adipopancreatic tissue of Atlantic herring Clupea harengus

“…In the last few years, accumulation of expert knowledge about fish cell culture, along with advances in aquaculture, have led to the establishment of several new cell lines from economically important fishes (Bols 1991, Bejar et al 1997, Segner 1998, Villena 2003, Ciba et al 2008, Fan et al 2010. Examples are caudal fin cells from gilt-head sea bream Sparus aurata (Bejar et al 1997), neural stem cells from adult seabass Dicentrarchus labrax (Servili et al 2009), muscle and fin cells of bluefin trevally Caranx melampygus (Zhao & Lu 2006), gill cells from rainbow trout ), and head kidney cells from Atlantic salmon Salmo salar (Dannevig et al 1995).…”