Long-term culture of integumental explants from premolt blue crabs,Callinectes sapidus

Abstract

SUMMARY: A technique is described for the contamination-free maintenance of blue crab (CaUinectes sapidus) premoh hypodermis in vitro for up to 36 days. Pre-exuvial integument is collected by sterile dissection from the animal after copious washing of the dorsal carapace with Betadyne solution and 70% ethanol. The tissue is cut into 3-to 5-ram 2 pieces while immersed in medium, then placed in culture dishes for 3 days in medium containing a broad range of antibiotic and antimycotic agents. After 3 days, the tissue is incubated with medium containing low antimicrobial chemical concentrations. This in vitro method will allow for the study of factors which influence the molt cycle.

Hepatopancreas cell cultures from mud crab, Scylla paramamosain

“…have been established. However, there are hardly any reports on culturing hepatopancreas cells from crabs (Ballard et al 1993;Sashikumar and Desai 2008).…”

Development of primary cell culture from Scylla serrata

“…Earlier studies on crab were related to cytotoxicology and histology Nott 1985, 1987). Ballard et al (1993) attempted integumentary cuticular epithelial cell culture of crab. However, there is hardly any report on development of primary cell cultures of commercially important crab species.…”

Susceptibility of testicular cell cultures of crab, Scylla serrata (Forskal) to white spot syndrome virus

“…Primary cell cultures from different shrimp tissues such as lymphoid organ (Chen and Kou 1989;Chen and Wang 1999;Wang et al 2000), heart (Chen and Wang 1999; Owens and Smith 1999), gut (Nadala et al 1993), ovaries (Maeda et al 2004) have been used to study various viral infections and replications but long surviving and proliferating cell cultures were not then available for evaluating viral infections. Though, few researchers have attempted primary cell cultures of crabs (Ballard et al 1993;Walton and Smith 1999;Sashikumar and Desai 2008;Zeng et al 2009) none have used crabs' cell culture for studying WSSV pathogenicity.…”