Long-term culture of primary breast cancer in defined medium

McCallum, H.; Lowther, G W

doi:10.1007/bf01806153

Cited by 35 publications

(36 citation statements)

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“…Over the years, new cell lines have been established from only a very small percentage of primary tumours (1-2%; Petersen et al, 1990;Band et al, 1990;Meltzer et al, 1991). However, a more recent report has described the long-term culture of approximately 7% of cultures established from primary breast cancers, nearly all of which went on to form new cell lines (McCallum and Lowther, 1996). Interestingly, the majority of these cultures grew in suspension, in contrast to most other studies, and were more likely to be established from grade III tumours with a steroid receptor-negative phenotype (McCallum and Lowther, 1996).…”

Section: Genetic Analysismentioning

confidence: 99%

“…However, a more recent report has described the long-term culture of approximately 7% of cultures established from primary breast cancers, nearly all of which went on to form new cell lines (McCallum and Lowther, 1996). Interestingly, the majority of these cultures grew in suspension, in contrast to most other studies, and were more likely to be established from grade III tumours with a steroid receptor-negative phenotype (McCallum and Lowther, 1996). However, in our study, pathoclinical features including tumour histology, grade, stage or node status had no bearing on culture success.…”

Section: Genetic Analysismentioning

confidence: 99%

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Short-term primary culture of epithelial cells derived from human breast tumours

Speirs

Green²,

Walton³

et al. 1998

Br J Cancer

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Summary As experimental models for breast cancer, most studies rely on established human breast cancer cell lines. However, many of these lines were established over 20 years ago, many from pleural effusions rather than the primary tumour, so the validity of using them as representative models is questionable. This paper describes our experiences, over a 3-year period, in establishing short-term epithelial-cellenriched preparations from primary breast tumours based on differential centrifugation followed by culture in selective media. Epithelial cells were successfully cultured from 55% of samples, but culture success did not appear to be correlated with tumour histology, stage, grade or node status. Epithelial cell-enriched cultures were immunopositive for broad-spectrum cytokeratin and epithelial membrane antigen (EMA). Positivity for keratin 19 confirmed that the cultures contained tumour-derived cells, which additionally showed significantly higher activity of the reductive pathway of the steroid-converting enzyme 17p-hydroxysteroid dehydrogenase type 1. That the cultures contained tumour and not normal epithelial cells was further substantiated by the complete absence of the calmodulin-like gene NB-1 in tumour-derived cultures; this is only associated with normal breast epithelia. Eighty-five per cent of cultures established from oestrogen receptor (ER)-positive tumours expressed ER in vitro; this was functional in 66% of cultures, although ER-positive phenotype was gradually lost over time. In conclusion, epithelial cells can be isolated and maintained as short-term cultures from primary breast tumours irrespective of histopathological or clinical details, providing a model system with a greater biological and clinical relevance than breast cancer cell lines.

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Section: Genetic Analysismentioning

confidence: 99%

Section: Genetic Analysismentioning

confidence: 99%

Short-term primary culture of epithelial cells derived from human breast tumours

Speirs

Green²,

Walton³

et al. 1998

Br J Cancer

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“…Human breast tumor cell lines, however, are difficult to establish in culture (Smith et al, , 1987. Moreover, breast tumor cells are frequently contaminated with normal epithelial, stromal or mesothelial cells that demonstrate initial in vitro growth, making it difficult to determine the source of the proliferating cultured cells (McCallum and Lowther, 1996). Although about 50-70 human breast cancer lines have been described in publications, the number of breast tumor cell lines that have been adequately characterized and are widely used is only about 20 .…”

Section: ^Vfth-nmosomalmentioning

confidence: 99%

“…Almost all of the primary tumor cell lines were derived from patients who also had nodal metastases . Additional problems with the use of currently available breast cancer cell lines include slow growth rates in vitro (McCallum and Lowther, 1996), and lack of hormonal responses. MCF-7 is the most widely studied breast carcinoma cell line because of its steroid receptor status and estrogen sensitivity (Levenson and Jordan, 1997), whereas other cell lines that have low steroid receptor expression (such as PMC42) are not widely used .…”

Section: ^Vfth-nmosomalmentioning

confidence: 99%

A Comprehensive Repository of Normal and Tumor Human Breast Tissues and Cells

Shay¹

1999

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302, and to the Office of Management and Budget, Paperwork Reduction Project (0704-0188), Washington, DC 20503. AGENCY USE ONLY (Leave blank)REPORT DATE July 1999 REPORT TYPE AND DATES COVEREDFinal (1 Jul 94 -30 Jun 99) TITLE AND SUBTITLE A Comprehensive Repository of Normal and Tumor Human BreastTissues and Cells AUTHOR(S)Jerry W. Shay, Ph.D.• DCDcnRMINR ORGANIZATION NAME(S) AND ADDRESS(ES)The University We have successfully established a breast tissue repository which contains over 250 individual breast specimens. In addition, we have cryopreserved and cultured cells from human breast tumor tissues and have established 21 new human breast tumor cell lines (including one with a BRCA-1 mutation). The repository also includes breast epithelial and stromal cell strains derived from non cancerous breast tissue as well as peripheral blood mononuclear cells. The new breast tumor cell lines have been deposited at the ATCC repository for unrestricted distribution. The rest of the respository is widely available for distribution at our world wide web homepage: http://www.swmed.edu/home_pages/bcrep/ with links to other breast tissue banks.14. SUBJECT TERMS Where copyrighted material is quoted, permission has been obtained to use such material. Breast CancerWhere material from documents designated for limited distribution is quoted, permission has been obtained to use the material.rJ / Citations of commercial organizations and trade names in this report do not constitute an official Department of Army endorsement or approval of the products or services of these organizations. Qt&Jfc JJ adhe:In conducting research using animals, the investigator(s) adhered to the "Guide for the Care and Use of Laboratory Animals," prepared by the Committee on Care and use of Laboratory Animals of the Institute of Laboratory Resources, national Research Council (NIH Publication No. 86-23, Revised 1985).For the protection of human subjects, the investigator(s) adhered to policies of applicable Federal Law 45 CFR 46. 5/In conducting research utilizing recombinant DNA technology, the investigator(s) adhered to current guidelines promulgated by the National Institutes of Health.In the conduct of research utilizing recombinant DNA, the investigator(s) adhered to the NIH Guidelines for Research Involving Recombinant DNA Molecules. %fIn the conduct of research involving hazardous organisms, the investigator(s) adhered to the CDC-NIH Guide for Biosafety in Microbiologica...

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“…However, selective culture of neoplastic cells and successful establishment of new cell lines have rarely been reported from primary breast carcinomas (2,3).…”

Section: Introductionmentioning

confidence: 99%

Glyceraldehyde-3-phosphate dehydrogenase as a surface associated antigen on human breast cancer cell lines MACL-1 and MGSO-3

Correa

Bertollo²,

Zouain³

et al. 2010

Oncol Rep

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Abstract. Breast cancer is a major health burden, responsible for >10% of all cases of cancer worldwide. Advances in breast cancer diagnosis and treatment have contributed to an improved rate of survival, although mortality rates remains significantly high. The establishment of breast cancer cell lines is an important model for understanding biological processes involved in this disease and for identifying potential therapeutic targets. The novel human breast cancer cell lines, MACL-1 and MGSO-3, were used in this study to identify possible surface antigens by antibodies directed against two commercial breast cancer cell lines MCF-7 and MDA-MB-231. We purified a 37 kDa antigen by affinity chromatography from MDA-MB-231, and its N-terminal amino acid sequence was homologous to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Therefore, immunohistochemical experiments, using specific monoclonal antibodies, evidenced a co-localization of GAPDH and Na + /K + -ATPase on the surface of commercially available and recently established breast cancer cell lines. It is of note that Na + /K + -ATPase was used as a plasma membrane marker. This finding opens new perspectives for breast cancer diagnosis and treatment since GAPDH could be used as a biomarker or as a potential therapeutic target in breast cancer. IntroductionCancer is a public health problem worldwide. Among the various types of cancer, breast cancer is the most common in women, in both high and low-resource settings, and is responsible for over one million of the estimated 10 million neoplasms diagnosed in both sexes worldwide each year (1). It is also the primary cause of death among women globally, responsible for ~375,000 deaths in the year 2000 (1). Although the 5-year survival rate has greatly increased as a result of advances in detection and treatment, many breast cancer patients still die from metastatic disease. Therefore, more effective methods of prevention and treatment are greatly needed.Cell cultures, established directly from patients, are an important model for examining and manipulating the potentially relevant molecules and cellular processes underlying malignant breast disease. However, selective culture of neoplastic cells and successful establishment of new cell lines have rarely been reported from primary breast carcinomas (2,3).The discovery of T-cell recognition and subsequent lysis of human tumors led to the identification of specific oncoproteins or mutated oncogenes (e.g., MUC-1, HER-2, CEA, and p53) (4-7). Many of these tumor antigens are found on normal tissues, but in cases of oncogenesis, are recognized by the immune system due to their overexpression. Some proteins are tumor-specific, but others, such as the universal tumor Ag hTERT, are broadly expressed by most tumor cells (8,9).In the present study, we describe a potential therapeutic target antigen on breast cancer cell lines, established from primary breast carcinoma by Correa et al (9). Using antibodies directed against the commercial cell lines MCF-7 and MDA-MB-231, we we...

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Long-term culture of primary breast cancer in defined medium

Cited by 35 publications

References 11 publications

Short-term primary culture of epithelial cells derived from human breast tumours

Short-term primary culture of epithelial cells derived from human breast tumours

A Comprehensive Repository of Normal and Tumor Human Breast Tissues and Cells

Glyceraldehyde-3-phosphate dehydrogenase as a surface associated antigen on human breast cancer cell lines MACL-1 and MGSO-3

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