Long-term cytokine and growth factor release from equine platelet-rich fibrin clots obtained with two different centrifugation protocols

Jimenez-Aristizabal, R.; López, Catalina; Álvarez, María E.; Giraldo, Carlos Eduardo; Prades, Marta; Carmona, JU

doi:10.1016/j.cyto.2017.06.011

Cited by 29 publications

(20 citation statements)

References 29 publications

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“…In addition, growth factors and cytokines are released from platelets over a longer period of time, whereas in PRP these factors are released immediately into the site of application [22,23]. Nevertheless, PRF is not capable of replacing PRP in all therapeutic areas because of its compact three-dimensional structure, which hinders its application as an injection [24,25,26]. Consequently, PRF is mainly used for replacing injured tissues in oral and dental surgery, orthopedics, and wound healing [27,28,29,30,31].…”

Section: Introductionmentioning

confidence: 99%

The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method

Kardos

Simon

Vácz

et al. 2019

IJMS

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Autologous blood derived products, such as platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are widely applied in regenerative therapies, in contrast to the drawbacks in their application, mainly deriving from the preparation methods used. Eliminating the disadvantages of both PRP and PRF, hyperacute serum (HAS) opens a new path in autologous serum therapy showing similar or even improved regenerative potential at the same time. Despite the frequent experimental and clinical use of PRP and HAS, their protein composition has not been examined thoroughly yet. Thus, we investigated and compared the composition of HAS, serum, PRP and plasma products using citrate and EDTA by simple laboratory tests, and we compared the composition of HAS, serum, EDTA PRP and plasma by Proteome Profiler and ELISA assays. According to our results the natural ionic balance was upset in both EDTA and citrate PRP as well as in plasma. EDTA PRP contained significantly higher level of growth factors and cytokines, especially platelet derived angiogenic and inflammatory proteins, that can be explained by the significantly higher number of platelets in EDTA PRP. The composition analysis of blood derivatives revealed that although the preparation method of PRP and HAS were similar, the ionic and protein composition of HAS could be advantageous for cell function.

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Section: Introductionmentioning

confidence: 99%

The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method

Kardos

Simon

Vácz

et al. 2019

IJMS

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“…Consequently, a storage pool of growth factors is formed from platelets upon activation. These molecules act as cell attractants and they increase the rate of the proliferation of adhered cells [ 15 ]. Serum squeezed out from the PRF clot, called hyperacute serum, has higher cell proliferative effect on bone marrow mesenchymal stem cells (MSCs), osteoblasts and osteoarthritic chondrocyte cells than PRP [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Biological and Mechanical Properties of Platelet-Rich Fibrin Membranes after Thermal Manipulation and Preparation in a Single-Syringe Closed System

Kardos

Hornyák

Simon

et al. 2018

IJMS

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Platelet-rich fibrin (PRF) membrane is a three-dimensional biodegradable biopolymer, which consists of platelet derived growth factors enhancing cell adhesion and proliferation. It is widely used in soft and hard tissue regeneration, however, there are unresolved problems with its clinical application. Its preparation needs open handling of the membranes, it degrades easily, and it has a low tensile strength which does not hold a suture blocking wider clinical applications of PRF. Our aim was to produce a sterile, suturable, reproducible PRF membrane suitable for surgical intervention. We compared the biological and mechanical properties of PRF membranes created by the classical glass-tube and those that were created in a single-syringe closed system (hypACT Inject), which allowed aseptic preparation. HypACT Inject device produces a PRF membrane with better handling characteristics without compromising biological properties. Freeze-thawing resulted in significantly higher tensile strength and higher cell adhesion at a lower degradation rate of the membranes. Mesenchymal stem cells seeded onto PRF membranes readily proliferated on the surface of fresh, but even better on freeze/thawed or freeze-dried membranes. These data show that PRF membranes can be made sterile, more uniform and significantly stronger which makes it possible to use them as suturable surgical membranes.

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“…This degradation occurred faster with increasing cell numbers within the matrix. After clot degradation different growth factors like TNF-α, TGF-β, PDGF, EGF or VEGF will be released and cell survival as well as cell proliferation is enhanced [82][83][84]. In general, the plasma clot represents a useful scaffold for transplantation due to many advantages and besides beneficial influence on OSCs, it can also be used as drug delivery system [85].…”

Section: Discussionmentioning

confidence: 99%

Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix

Rövekamp¹,

Volkenstein²,

Minovi³

et al. 2020

RGM

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Cell therapies represent promising strategies to improve neurological functions after spinal cord injury (SCI). Olfactory mucosa (OM) might be an attractive source of multipotent cells for neuroregeneration because olfactory stem cells (OSCs) are resident. The regenerative capacity of OSCs has been demonstrated in animal models and some clinical case reports. Up to now, there are no standard methods for purification, characterization, and delivery of OSCs to the injury site. However, purification and characterization of the grafted cells are prerequisites for clinical use to ensure maximum safety for the patients. In this study, we isolated and purified OSCs from human OM using the neurosphere assay. Subsequently, the cells were characterized, and the behavior of purified OSCs in a plasma clot was investigated. Our study demonstrated that isolated cells from OM form neurospheres, which cells are positive for CD105 (98%) and CD90 (99%) and negative for Epcam (<1%) and MUC5AC (<1%). Purified OSCs were positive for Nestin, CD44 as well as GFAP and showed a lack of CD34 and CD45 expression. OSCs differentiated into neuron-like cells expressing ß-III tubulin. However, differentiation into adipocytes, chondrocytes or osteoblast could not be observed. In addition, OSCs stayed viable and were able to proliferate within the plasma clot. These results highlight OSC as a candidate for autologous transplantation in combination with the plasma clot as a cell carrier in SCI and neurodegenerative disease.

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Long-term cytokine and growth factor release from equine platelet-rich fibrin clots obtained with two different centrifugation protocols

Cited by 29 publications

References 29 publications

The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method

The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method

Biological and Mechanical Properties of Platelet-Rich Fibrin Membranes after Thermal Manipulation and Preparation in a Single-Syringe Closed System

Olfactory Stem Cells for the Treatment of Spinal Cord Injury: Isolation, Purification and Behaviour in a Plasma Clot Matrix

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