Long-term effects of cryopreservation on clinically prepared hematopoietic progenitor cell products

Winter, Jacob M.; Jacobson, Pam; Bullough, Brandon; Christensen, Austin P.; Boyer, Michael W.; Reems, Jo Anna

doi:10.1016/j.jcyt.2014.02.005

Cited by 27 publications

(10 citation statements)

References 31 publications

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“…This is achieved as cellular, chemical and biological processes in the sample effectively stop below this temperature [32]. Allowing the storage conditions to rise above the glass transition temperature, even briefly, introduces the risk of resumed diffusion, threatening the stability of the samples [38][39][40]. It should also be noted that the trypan blue assay gave a higher level of viability than flow cytometry in this instance, as was the case when assessing the effect of gender on post-that performance (Fig 5).…”

Section: Plos Onementioning

confidence: 99%

Automated dry thawing of cryopreserved haematopoietic cells is not adversely influenced by cryostorage time, patient age or gender

et al. 2020

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Cell therapies are becoming increasingly widely used, and their production and cryopreservation should take place under tightly controlled GMP conditions, with minimal batch-to-batch variation. One potential source of variation is in the thawing of cryopreserved samples, typically carried out in water baths. This study looks at an alternative, dry thawing, to minimise variability in the thawing of a cryopreserved cell therapy, and compares the cellular outcome on thaw. Factors such as storage time, patient age, and gender are considered in terms of cryopreservation and thawing outcomes. Cryopreserved leukapheresis samples from 41 donors, frozen by the same protocol and stored for up to 17 years, have been thawed using automated, water-free equipment and by conventional wet thawing using a water bath. Post-thaw viability, assessed by both trypan blue and flow cytometry, showed no significant differences between the techniques. Similarly, there was no negative effect of the duration of frozen storage, donor age at sample collection or donor gender on post-thaw viability using either thawing method. The implication of these results is that the cryopreservation protocol chosen initially remains robust and appropriate for use with a wide range of donors. The positive response of the samples to water-free thawing offers potential benefits for clinical situations by removing the subjective element inherent in water bath thawing and eliminating possible contamination issues.

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Section: Plos Onementioning

confidence: 99%

Automated dry thawing of cryopreserved haematopoietic cells is not adversely influenced by cryostorage time, patient age or gender

et al. 2020

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“…By focusing on parameters potentially affecting the rate of CD34 + cells caspases activation, we find an impact on post thawing graft level of granulocytes whereas patients diseases do not seem to have of influence. A significant negative correlation was previously determined by Winter et al, between pre-freeze percent granulocyte content and post-thaw percent viability of TNCs, not specifically targeting CD34 + cells 21 . Those observations might be related to degranulation of granulocytes probably occurs during thawing.…”

Section: Discussionmentioning

confidence: 59%

Cryopreservation and thawing of hematopoietic stem cell CD34-induced apoptosis through caspase pathway activation: Key role of granulocytes

et al. 2019

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“…In addition to the quantification of CD34þ cells, determination of clonogenic potential represents another standard test for the evaluation of graft quality. Some authors showed decreased CFUs in PBSCs or cord blood units after cryopreservation and thawing 38,39 . Regardless, CFU recoveries remain high and do not change significantly with storage time.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Cryopreservation Does Not Affect Quality of Peripheral Blood Stem Cell Grafts: A Comparative Study of Native, Short-Term and Long-Term Cryopreserved Haematopoietic Stem Cells

Lysák¹,

Brychtová

Leba

et al. 2021

Cell Transplant

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Cryopreserved haematopoietic progenitor cells are used to restore autologous haematopoiesis after high dose chemotherapy. Although the cells are routinely stored for a long period, concerns remain about the maximum storage time and the possible negative effect of storage on their potency. We evaluated the effect of cryopreservation on the quality of peripheral stem cell grafts stored for a short (3 months) and a long (10 years) period and we compared it to native products.The viability of CD34+ cells remained unaffected during storage, the apoptotic cells were represented up to 10% and did not differ between groups. The clonogenic activity measured by ATP production has decreased with the length of storage (ATP/cell 1.28 nM in native vs. 0.63 in long term stored products, P < 0.05). Only borderline changes without statistical significance were detected when examining mitochondrial and aldehyde dehydrogenase metabolic activity and intracellular pH, showing their good preservation during cell storage. Our experience demonstrates that cryostorage has no major negative effect on stem cell quality and potency, and therefore autologous stem cells can be stored safely for an extended period of at least 10 years. On the other hand, long term storage for 10 years and longer may lead to mild reduction of clonogenic capacity. When a sufficient dose of stem cells is infused, these changes will not have a clinical impact. However, in products stored beyond 10 years, especially when a low number of CD34+ cells is available, the quality of stem cell graft should be verified before infusion using the appropriate potency assays.

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Long-term effects of cryopreservation on clinically prepared hematopoietic progenitor cell products

Cited by 27 publications

References 31 publications

Automated dry thawing of cryopreserved haematopoietic cells is not adversely influenced by cryostorage time, patient age or gender

Automated dry thawing of cryopreserved haematopoietic cells is not adversely influenced by cryostorage time, patient age or gender

Cryopreservation and thawing of hematopoietic stem cell CD34-induced apoptosis through caspase pathway activation: Key role of granulocytes

Long-Term Cryopreservation Does Not Affect Quality of Peripheral Blood Stem Cell Grafts: A Comparative Study of Native, Short-Term and Long-Term Cryopreserved Haematopoietic Stem Cells

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