Long-term effects of oxytetracycline exposure in zebrafish: A multi-level perspective

Almeida, Ana Rita; Tacão, Marta; Machado, Ana Luísa; Golovko, Oksana; Žlábek, Vladimír; Domingues, Inês; Henriques, Isabel

doi:10.1016/j.chemosphere.2019.01.147

Cited by 81 publications

(59 citation statements)

References 77 publications

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“…However, various sublethal effects were observed at low, environmentally realistic concentrations of antibiotics. Almeida et al [ 110 ] reported enhanced exploratory behavior and stress response in Danio rerio after long-term exposure to low oxytetracycline concentrations. The authors supposed that antibiotic increased energy consumption and reduced lipid level.…”

Section: Toxic Effects Of Antibacterials On Fish Organismsmentioning

confidence: 99%

Antibacterials in Aquatic Environment and Their Toxicity to Fish

2020

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Antibacterial agents are commonly present in aquatic environment at low concentrations. Terrestrial animal farms, human medicine and aquaculture are main sources of water contamination with antibacterials. Antibiotics were proved to be directly toxic to fish causing oxidative stress, general stress response, histopathological lesions, hematological, metabolic, and reproductive disorders, as well as immunosuppressive and genotoxic effects. Environmentally realistic low concentrations of antibiotics also disturb aquatic bacterial communities causing alterations in fish symbiotic microbiota and induce emergence of antibiotic-resistant pathogenic bacteria by exerting selective pressure on spread of antibiotic-resistance genes.

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Section: Toxic Effects Of Antibacterials On Fish Organismsmentioning

confidence: 99%

Antibacterials in Aquatic Environment and Their Toxicity to Fish

2020

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“…The situation becomes much worse when aquafarmers use antibiotics incorporated into feed at subtherapeutic doses as prophylactics and as growth promoters rather than using as therapeutics (Bush et al, ). It was reported that majority of these antibiotics are not completely metabolized by the treated organism and thereby discharged into the waterbody, making aquaculture responsible for contaminating the environment (Almeida et al, ; Burridge, Weis, Cabello, Pizarro, & Bostick, ). Oxytetracycline (OTC) is one such broad spectrum antibiotic from tetracycline family that is low‐cost compared to other antibiotics and represents nearly one‐third of the antibiotic consumption in animal production in the EU (Almeida et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…It was reported that majority of these antibiotics are not completely metabolized by the treated organism and thereby discharged into the waterbody, making aquaculture responsible for contaminating the environment (Almeida et al, ; Burridge, Weis, Cabello, Pizarro, & Bostick, ). Oxytetracycline (OTC) is one such broad spectrum antibiotic from tetracycline family that is low‐cost compared to other antibiotics and represents nearly one‐third of the antibiotic consumption in animal production in the EU (Almeida et al, ). Although its use as growth promoter is not permitted in the EU and the United States, its use is still allowed in many countries (Van Boeckel et al, ).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Oxytetracycline (OTC) is one such broad spectrum antibiotic from tetracycline family that is low-cost compared to other antibiotics and represents nearly one-third of the antibiotic consumption in animal production in the EU (Almeida et al, 2019). Although its use as growth promoter is not permitted in the EU and the United States, its use is still allowed in many countries (Van Boeckel et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial activity of oxytetracycline on microbial ecology of Nile tilapia ( Oreochromis niloticus ) gastrointestinal tract under laboratory condition

et al. 2020

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The antibacterial effect of oxytetracycline (OTC) on bacterial load, pathogen and possible development of antibiotic resistance in intestinal flora of apparently healthy Nile tilapia (Oreochromis niloticus) was investigated under laboratory condition. OTC, a broad spectrum approved antibiotic widely used in aquaculture, was fed to Nile tilapia through medicated diet at a rate of 2 g/ kg of feed for consecutive 7 days in treatment 1 (T1) and 30 days in treatment 2 (T2). Changes in physico‐chemical parameters of water were also recorded where pH and dissolved oxygen influenced bacterial load and coliform count respectively. At the start of the experiment, initial bacterial load in fish intestine was 1.61 ± 2.25 × 1010 cfu/g, which after OTC treatment decreased significantly (p < .05) to 3.06 ± 2.08 × 108 cfu/g on day 7 and 3.45 ± 4.46 × 107 cfu/g on day 30 when compared with the control group. Among the 10 bacterial genera identified in the intestine, 4 potential public health concern bacteria viz., Salmonella, Escherichia, Enterobacter and Staphylococcaus were predominant. There was a sharp increase in bacterial load on day 18 in T2 that may be attributed to the development of antibiotic resistance in intestinal bacteria. Antibiotic susceptibility test for isolates against seven antibiotics: amoxycillin, ciprofloxacin, co‐trimoxazole, erythromycin, gentamicin, nitrofurantoin and tetracycline using disc diffusion method revealed significantly increased resistance of Gram‐negative rods (p < .05) that possibly caused increased frequency of OTC‐resistant microorganisms. In conclusion, short‐ and long‐term exposure to OTC treatment affected the distribution of bacterial genera including pathogens in the Nile tilapia gastrointestinal tract and concomitantly influenced their antimicrobial resistance.

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Application of zebrafish in the study of the gut microbiome

Zhong

et al. 2022

Anim Models and Exp Med

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The gut microbiome is widely acknowledged to coexist with the animal host, consisting of symbiotic and pathogenic bacteria in a dynamic balance state that produce a wide variety of signaling molecules. These bacteria colonize the intestine and perform functions the host itself cannot accomplish; in turn, they rely on the habitat provided by the host. A healthy gut microbiome plays a significant and irreplaceable role in determining the host's overall health.Zebrafish is an omnivorous freshwater fish that belongs to the small carp family. It has been widely used for research purposes on embryology and tissue regeneration, molecular genetics, reproductive biology, and toxicology given its numerous advantages, including high fecundity, short lifespan, highly annotated genome, optical

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Long-term effects of oxytetracycline exposure in zebrafish: A multi-level perspective

Cited by 81 publications

References 77 publications

Antibacterials in Aquatic Environment and Their Toxicity to Fish

Antibacterials in Aquatic Environment and Their Toxicity to Fish

Antibacterial activity of oxytetracycline on microbial ecology of Nile tilapia ( Oreochromis niloticus ) gastrointestinal tract under laboratory condition

Application of zebrafish in the study of the gut microbiome

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