2019
DOI: 10.15252/embj.2018100300
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Long‐term expanding human airway organoids for disease modeling

Abstract: Organoids are self‐organizing 3D structures grown from stem cells that recapitulate essential aspects of organ structure and function. Here, we describe a method to establish long‐term‐expanding human airway organoids from broncho‐alveolar resections or lavage material. The pseudostratified airway organoids consist of basal cells, functional multi‐ciliated cells, mucus‐producing secretory cells, and CC10‐secreting club cells. Airway organoids derived from cystic fibrosis (CF) patients allow assessment of CFTR … Show more

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Cited by 747 publications
(896 citation statements)
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References 75 publications
(104 reference statements)
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“…Since then, organoid cultures have been established for a variety of human tissues, including lung (Hild & Jaffe, ; Tan et al , ; Sachs et al , ), colon (Sato et al , ), stomach (Bartfeld et al , ), liver (Huch et al , ), pancreas (Boj et al , ), prostate (Chua et al , ; Karthaus et al , ), kidney (Jun et al , ; Schutgens et al , ) and fallopian tube (Kessler et al , ). Moreover, organoid culture protocols have been established for patient‐derived tumour tissue as well.…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Since then, organoid cultures have been established for a variety of human tissues, including lung (Hild & Jaffe, ; Tan et al , ; Sachs et al , ), colon (Sato et al , ), stomach (Bartfeld et al , ), liver (Huch et al , ), pancreas (Boj et al , ), prostate (Chua et al , ; Karthaus et al , ), kidney (Jun et al , ; Schutgens et al , ) and fallopian tube (Kessler et al , ). Moreover, organoid culture protocols have been established for patient‐derived tumour tissue as well.…”
Section: Introductionmentioning
confidence: 99%
“…Moreover, organoid culture protocols have been established for patient‐derived tumour tissue as well. Human tumour organoids have been generated from colon (Sato et al , ; van de Wetering et al , ), pancreas (Boj et al , ; Huang et al , ), prostate (Gao et al , ; Drost et al , ), breast (Sachs et al , ), gastric (Nanki et al , ; Yan et al , ), lung (Sachs et al , ), oesophageal (Li et al , ), bladder (Lee et al , ; Mullenders et al , ), ovarian (Kopper et al , ), kidney (Schutgens et al , ) and liver (Broutier et al , ; Li et al , ) tumour tissue (Fig ). An important feature of a number of these PDTOs is that they genetically and phenotypically mirror the tumour epithelium, including its intra‐tumour heterogeneity (Huang et al , ; van de Wetering et al , ; Nanki et al , ; Sachs et al , ; Yan et al , ).…”
Section: Introductionmentioning
confidence: 99%
“…Currently, organoids are used as models of different pathologies, including infectious diseases caused by viruses, bacteria, and protozoans [3][4][5]. Viral infection studies with organoid systems have included: norovirus, rotavirus, enteric adenovirus, and coronavirus invasion of intestinal organoids [3][4][5][6]; herpes simplex virus 1 [23] and cytomegalovirus [24] infection of cerebral organoids; Zika virus infection of cerebral [4,5] and human testicular organoids [25]; human airway organoids to model pathology and assess infectivity of emerging influenza [26], parainfluenza [27] and respiratory syncytial viruses [28]; BK virus infection in human kidney tubuloids [29]; and human liver organoids to study infection with hepatitis B and its related tumorigenesis [30].…”
Section: Organoids: What Whence and Where To Infection Biologymentioning
confidence: 99%
“…In addition to surgically removed human lung tissue, organoids can be generated from the culture of human lung epithelial cells collected from bronchoalveolar lavage fluid (BALF) or rectal biopsies for certain purposes [46]. Isolated human lung stem/progenitor cells may be cryopreserved and thawed later for organoid culture [47].…”
Section: Development Of Alveolar Organoidsmentioning
confidence: 99%