Long-Term Expanding Porcine Airway Organoids Provide Insights into the Pathogenesis and Innate Immunity of Porcine Respiratory Coronavirus Infection

Jiang, Chengfan; Li, Liang; Xue, Mei; Zhao, Liyuan; Liu, Xiang; Wang, Wenzhe; Feng, Li; Liu, Pinghuang

doi:10.1128/jvi.00738-22

Cited by 8 publications

(3 citation statements)

References 36 publications

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“…Additionally, the protective effect of pig milk small extracellular vesicles (sEV) against PEDV was validated in the organoid model, aligning with observations from cell lines and in vivo experiments [ 72 ]. Furthermore, an extended pig airway organoid, capable of sustaining long-term cultures, was developed for the study of porcine respiratory coronavirus (PRCoV) [ 73 ]. Both 3D and 2D airway organoids proved permissive for PRCoV infection, establishing versatile platforms for studying respiratory coronavirus dynamics and test therapeutic strategies.…”

Section: Discussionmentioning

confidence: 99%

Mapping the scientific output of organoids for animal and human modeling infectious diseases: a bibliometric assessment

Yan,

Monlong,

Cougoule

et al. 2024

Vet Res

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The escalation of antibiotic resistance, pandemics, and nosocomial infections underscores the importance of research in both animal and human infectious diseases. Recent advancements in three-dimensional tissue cultures, or “organoids”, have revolutionized the development of in vitro models for infectious diseases. Our study conducts a bibliometric analysis on the use of organoids in modeling infectious diseases, offering an in-depth overview of this field’s current landscape. We examined scientific contributions from 2009 onward that focused on organoids in host‒pathogen interactions using the Web of Science Core Collection and OpenAlex database. Our analysis included temporal trends, reference aging, author, and institutional productivity, collaborative networks, citation metrics, keyword cluster dynamics, and disruptiveness of organoid models. VOSviewer, CiteSpace, and Python facilitated this analytical assessment. The findings reveal significant growth and advancements in organoid-based infectious disease research. Analysis of keywords and impactful publications identified three distinct developmental phases in this area that were significantly influenced by outbreaks of Zika and SARS-CoV-2 viruses. The research also highlights the synergistic efforts between academia and publishers in tackling global pandemic challenges. Through mostly consolidating research efforts, organoids are proving to be a promising tool in infectious disease research for both human and animal infectious disease. Their integration into the field necessitates methodological refinements for better physiological emulation and the establishment of extensive organoid biobanks. These improvements are crucial for fully harnessing the potential of organoids in understanding infectious diseases and advancing the development of targeted treatments and vaccines.

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Section: Discussionmentioning

confidence: 99%

Mapping the scientific output of organoids for animal and human modeling infectious diseases: a bibliometric assessment

Yan,

Monlong,

Cougoule

et al. 2024

Vet Res

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“…Porcine respiratory coronavirus (PRCoV), a naturally occurring spike deletion mutant of highly enteropathogenic transmissible gastroenteritis virus (TGEV), could be a surrogate to study the pathogenesis of human respiratory coronaviruses. Long-term 3D porcine airway organoids (AOs) derived from basal epithelial cells and AO-derived monolayer cultures were generated and provided insights into the pathogenesis and innate immunity of PRCoV ( 27 ). In these models, 3D AOs consists of four major types of airway epithelial cells identified by immunostaining, including ciliated cells, goblet cells, basal cells, and club cells.…”

Section: Organoid Models For Veterinary Infectious Diseasesmentioning

confidence: 99%

“…To date and to the best of our knowledge, organoid technology has modeled 12 veterinary infectious diseases, including infections caused by viruses [caprine arthritis encephalitis virus (26), swine pulmonary and enteric coronavirus (27)(28)(29)(30), feline coronavirus (56) and rabbit calicivirus (36)], bovine rotavirus (Group A rotaviruses) (32), bacteria (Lawsonia intracellularis (33), Salmonella typhimurium (34) and Enterotoxigenic E. coli (35)), and a parasite [Toxoplasma gondii (34)]. Detailed information on organoid models for specific infectious diseases is listed in Table 1.…”

Section: Organoid Models For Veterinary Infectious Diseasesmentioning

confidence: 99%

Advances in organoid technology for veterinary disease modeling

Chen,

Slocombe,

Georgy

2023

Front. Vet. Sci.

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Organoids are in vitro organ-like structures that faithfully recapitulate many characteristics of a specific organ. During the past decades, major progress has been accomplished in establishing three-dimensional (3D) culture systems toward stem cell-derived organoids. As a significant technological breakthrough, these amazing 3D organoid constructs bridge the conventional 2D in vitro models and in vivo animal models and provide an unprecedented opportunity to investigate the complexities of veterinary diseases ranging from their pathogenesis to the prevention, therapy, or even future organ replacement strategies. In this review, we briefly discuss several definitions used in organoid research and highlight the currently known achievements in modeling veterinary diseases, including infectious and inflammatory diseases, cancers, and metabolic diseases. The applications of organoid technology in veterinary disease modeling are still in their infancy stage but the future is promising.

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Advancing zoonotic respiratory virus research through the use of organoids

Flagg,

de Wit

2024

Current Opinion in Virology

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Long-Term Expanding Porcine Airway Organoids Provide Insights into the Pathogenesis and Innate Immunity of Porcine Respiratory Coronavirus Infection

Cited by 8 publications

References 36 publications

Mapping the scientific output of organoids for animal and human modeling infectious diseases: a bibliometric assessment

Mapping the scientific output of organoids for animal and human modeling infectious diseases: a bibliometric assessment

Advances in organoid technology for veterinary disease modeling

Advancing zoonotic respiratory virus research through the use of organoids

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