Long-term exposure to environmental levels of phenanthrene disrupts spermatogenesis in male mice

Huang, Jie; Fang, Lü; Zhang, Shenli; Zhang, Ying; Ou, Kunlin; Wang, Chonggang

doi:10.1016/j.envpol.2021.117488

Cited by 21 publications

(11 citation statements)

References 70 publications

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“…Previous studies showed the same results as our study of PI3K-Akt signalling pathway regulated spermatogenesis. [40][41][42] Aquila et al study found that estradiol could enhance phosphorylation of the protein Akt, which was regarded as a germ cell survival factor in the human testis. 43 Dube et al…”

Section: Discussionmentioning

confidence: 99%

Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction

et al. 2022

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Objectives:To evaluate the expression, potential functions and mechanisms of long noncoding RNAs (lncRNAs) in the pathogenesis of varicocele (VC)-induced spermatogenic dysfunction. Materials and Methods:We established a rat model with left experimental VC and divided rats into the sham group, the VC group, and the surgical treatment group (each group, n = 10). Haematoxylin and eosin (HE) staining and sperm quality were analysed to evaluate spermatogenesis function. LncRNA expression profiles were analysed using lncRNA-Seq (each group n = 3) and validated using quantitative realtime polymerase chain reaction (each group n = 10). Correlation analysis and gene target miRNA prediction were used to construct competing endogenous RNA network.The regulated signalling pathway and spermatogenic dysfunction of differentially expressed lncRNAs (DE lncRNAs) were validated by Western blot.Results: HE detection and sperm quality analysis showed that VC could induce spermatogenic dysfunction. Eight lncRNAs were upregulated and three lncRNAs were downregulated in the VC group compared with the sham group and surgical treatment group. The lncRNA of NONRATG002949.

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Section: Discussionmentioning

confidence: 99%

Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction

et al. 2022

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“…It has been abundantly demonstrated that PHE exposure led to increase of apoptosis in cells and tissues by increasing the activity of caspase 3 and caspase 9 enzymes and Bax expression. 14,18 And the apoptosis is most likely due to oxidative stress in tissues caused by PHE. 15,61 In line with this, our result demonstrated that PHE induced oxidative stress and apoptosis in ovary, which may explain the low quality of oocyte after exposure of PHE in vivo.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…PHE exposure increased the level of apoptosis in testicle cells and altered the expression levels of oestrogen receptor, androgen receptor and aromatic hydrocarbon receptor in male mice. 18 It has been shown that PHE exposure increased follicular atresia and inhibited ovarian development by disrupting the function of hypothalamus-pituitary-gonadal (HPG) axis and the steroidogenic pathway aquatic organisms. [19][20][21] In addition, gestational exposure to PHE caused reproductive disorders in F1 adult female mice, which exhibited by the increased number of antral follicles and reduced levels of luteinizing hormone (LH) and estradiol in serum.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, long‐term exposure to environmental levels of PHE reduced the number of sperm and Sertoli cells. PHE exposure increased the level of apoptosis in testicle cells and altered the expression levels of oestrogen receptor, androgen receptor and aromatic hydrocarbon receptor in male mice 18 . It has been shown that PHE exposure increased follicular atresia and inhibited ovarian development by disrupting the function of hypothalamus–pituitary–gonadal (HPG) axis and the steroidogenic pathway aquatic organisms 19–21 .…”

Section: Introductionmentioning

confidence: 99%

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Exposure to phenanthrene affects oocyte meiosis by inducing mitochondrial dysfunction and endoplasmic reticulum stress

Wang¹,

Li²,

Yang³

et al. 2022

Cell Proliferation

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Objectives: Phenanthrene (PHE) is one of the most abundant polycyclic aromatic hydrocarbons (PAHs), which is a widespread environmental contaminant. Various studies showed that PHE has adverse impacts on animals and human health. It has been shown that PHE exposure induced follicular atresia and endocrine dyscrasia in female mice. However, the potential mechanism regarding how PHE affects female reproductive system especially the oocyte quality has not been elucidated. Methods and Results:In this study, we set up PHE exposure model and found that PHE exposure compromised oocytes maturation competence by inhibiting spindle assembly and chromosomes alignment. Moreover, PHE exposure induced mitochondrial dysfunction and endoplasmic reticulum (ER) stress, leading to increased reactive oxygen species (ROS) and aberrant calcium levels in cytoplasm, eventually induced oxidative stress and DNA damage in oocytes. Furthermore, we found that oral administration of PHE caused the occurrence of oxidative stress and apoptosis in female ovary. In addition, the oocyte exhibited aberrant spindle morphology and failure of actin cap formation in metaphase II oocytes. Conclusions:Taken together, our study demonstrated that mitochondrial dysfunction and ER stress-induced oxidative stress and DNA damage are the major cause of poor oocyte quality after PHE exposure.

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Chronic exposure to low levels of phenanthrene induces histological damage and carcinogenic risk in the uterus of female mice

Huang,

Zhang,

Fang

et al. 2024

Environ Sci Pollut Res

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Long-term exposure to environmental levels of phenanthrene disrupts spermatogenesis in male mice

Cited by 21 publications

References 70 publications

Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction

Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction

Exposure to phenanthrene affects oocyte meiosis by inducing mitochondrial dysfunction and endoplasmic reticulum stress

Chronic exposure to low levels of phenanthrene induces histological damage and carcinogenic risk in the uterus of female mice

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