Long‐Term Field Fertilization Significantly Alters Community Structure of Ammonia‐Oxidizing Bacteria rather than Archaea in a Paddy Soil

Wu, Yucheng; Lu, Lu; Wang, Baozhan; Lin, Xiangui; Zhu, Jiang; Cai, Zucong; Yan, Xiaoyuan; Jia, Zhongjun

doi:10.2136/sssaj2010.0434

Cited by 124 publications

(89 citation statements)

References 45 publications

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“…Furthermore, there was no significant difference in the population size of AOB among fertilizer treatments with the exception of the MV treatment and CK. This was inconsistent with previous studies that showed the population size of AOB increased significantly after fertilization (Wu et al, 2011;Ai et al, 2013;Strauss et al, 2014). Compared with AOB, the population size of AOA responded to fertilizers in a different manner (Figure 2).…”

Section: Discussioncontrasting

confidence: 99%

“…These findings are inconsistent with the results of some previous studies in which only Nitrosospira and not Nitrosomonas spp. were detected in soil (Chu et al, 2007;Wu et al, 2011). It is likely caused by the differences in soil type which is the primary determinants of the composition of the bacterial communities in arable soils (Girvan et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…They reported that the AOB abundance was more abundant in the N fertilization treatments than the control, AOA abundance decreased with increasing N fertilization rates. Glaser et al (2010) and Wu et al (2011) suggested that bacteria rather than archaea dominate ammonia oxidation in nearneutral or alkaline soils and in acidic soils, AOA play a more important role in ammonia oxidization (Zhang et al, 2012). From above literature reviews, it demonstrated that AOB and AOA may prefer to different ecological environments.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil

Fang

Yan

Chen

et al. 2015

Chilean J. Agric. Res.

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Ammonia oxidization is a critical step in the soil N cycle and can be affected by the fertilization regimes. Chinese milkvetch (Astragalus sinicus L., MV) is a major green manure of rice (Oryza sativa L.) fields in southern China, which is recommended as an important agronomic practice to improve soil fertility. Soil chemical properties, abundance and community structures of ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) in a MV-rice rotation field under different fertilization regimes were investigated. The field experiment included six treatments: control, without MV and chemical fertilizer (CK); 100% chemical fertilizer (NPK); 18 000 kg MV ha -1 plus 100% chemical fertilizer (NPKM1); 18 000 kg MV ha -1 plus 40% chemical fertilizer (NPKM2); 18 000 kg MV ha -1 alone (MV); and 18 000 kg MV ha -1 plus 40% chemical fertilizer plus straw (NPKMS). Results showed that NPKMS treatment could improve the soil fertility greatly although the application of 60% chemical fertilizer. The abundance of AOB only in the MV treatment had significant difference with the control; AOA were more abundant than AOB in all corresponding treatments. The NPKMS treatment had the highest AOA abundance (1.19 × 10 8 amoA gene copies g -1 ) and the lowest abundance was recorded in the CK treatment (3.21 × 10 7 amoA gene copies g -1 ). The abundance of AOA was significantly positively related to total N, available N, NH4 + -N, and NO3 --N. The community structure of AOA exhibited little variation among different fertilization regimes, whereas the community structure of AOB was highly responsive. Phylogenetic analysis showed that all AOB sequences were affiliated with Nitrosospira or Nitrosomonas and all AOA denaturing gradient gel electrophoresis (DGGE) bands belonged to the soil and sediment lineage. These findings could be fundamental to improve our understanding of AOB and AOA in the N cycle in the paddy soil.Key words: Abundance, ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB), Astragalus sinicus, community structure, Chinese milk vetch, soil chemical properties. INTRODUCTIONAppropriate fertilizer application is an important management practice to improve soil fertility. In recent years, due to the rapid population growth and continuous decline in the amount of cultivated land area, the application rate of fertilizer keeps on rising in tropical and subtropical regions of China in order to obtain high crop production. However, the long-term inappropriate fertilization has caused many environmental problems, such as high acidity, low nutrients in cultivated land and unbalanced ecosystem (Chen et al., 2010). Recently, soil quality has gained much attention as a result of environmental issues related to soil degradation and production sustainability under different farming systems (Galantini and Rosell, 2006). Chinese milk vetch (Astragalus sinicus L., MV) is considered as the most popular green manure in paddy field of South China, due to its high N-fixing potential and better growth under wet paddy soi...

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil

Fang

Yan

Chen

et al. 2015

Chilean J. Agric. Res.

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“…3a), which was in agreement with previous studies in a large number of agricultural soils (Wu et al 2011;Habteselassie et al 2013;Chen et al 2015). It has been reported that AOB growth is favored by high-N soil conditions (Jia and Conrad 2009;Verhamme et al 2011;Taylor et al 2012), whereas AOA growth is not affected (Di et al 2009(Di et al , 2010a.…”

Section: Nitrosospira Nitrososmonassupporting

confidence: 92%

Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Wang

Zhang

Shen

et al. 2016

Environ Sci Pollut Res

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Ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) are crucial for N 2 O emission as they carry out the key step of nitrification. Dicyandiamide (DCD) and acetylene (C 2 H 2 ) are typical nitrification inhibitors (NIs), while the comparative effects of these NIs on N 2 O production and ammonia oxidizers' (AOB and AOA) growth are unclear. Four treatments including a control, urea, urea + DCD, and urea + C 2 H 2 were set up to investigate their effect of inhibiting soil nitrification, nitrification-related N 2 O emission as well as the growth of ammonia oxidizers with a fluvo-aquic soil using microcosms for 28 days. N 2 O emission and net nitrification rate increased after the application of urea, but were significantly restrained in urea + NI treatments, while C 2 H 2 was more effective in reducing N 2 O emission and nitrification rate than DCD. The abundance of AOB, which was significantly correlated with N 2 O emission and net nitrification rate, was more inhibited by C 2 H 2 than DCD. Furthermore, the application of urea in all the soils had little impact on the AOA community, while obvious shifts of AOB community structure were found compared with the control. All AOB sequences fell within Nitrosospira cluster 3, and the AOA community was clustered to group 1.1b. Collectively, it indicated that application of urea combined with NIs (DCD or C 2 H 2 ) could potentially alter N 2 O emission, mainly through regulating the growth of AOB but not AOA in this fluvo-aquic soil.

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“…AOA did not respond to the application of NH 4 + in the alluvial soil, while it was inhibited with respect to the control in the red soil, thus confirming what it was obtained in agricultural soils (Wu et al 2011;Ai et al 2013;Habteselassie et al 2013;Di et al 2014Di et al , 2014Chen et al 2015). Generally, AOB growth is favored by high NH 4 + -N soil conditions (Jia and Conrad 2009;Verhamme et al 2011;Taylor et al 2012), whereas AOA growth is not affected or suppressed (Di et al 2009(Di et al , 2010Di and Cameron 2011;Di et al 2014).…”

Section: Discussionsupporting

confidence: 76%

Nitrogen fertiliser-induced changes in N2O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

et al. 2016

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Nitrification is believed to be one of the major sources of N 2 O production emitted from soil. Previous studies showed that both ammonia-oxidising bacteria (AOB) and archaea (AOA) can produce N 2 O via nitrification but their relative contributions are still poorly defined. Here, we used acetylene, an inhibitor of AOB and AOA ammonia monooxygenase (AMO), and 1-octyne, a selective inhibitor that specifically inhibits AOB AMO, to investigate how AOB versus AOA contribute to N 2 O emissions in two distinct arable soils. Soil amended with ammonium (NH 4 + ) increased N 2 O emissions to a greater extent than nitrate (NO 3 − ), and acetylene had a greater impact on N 2 O emissions in NH 4 + -treated soils than that in NO 3 − -amended soils, which indicated that nitrification was the dominant N 2 O emitting process in these two arable soils. In the alluvial and red soil, the percentage of evolved N 2 O after application of NH 4 + by AOB were 70.5~78.1 % and 18.7~19.7 % by AOA, respectively. Quantitative PCR revealed that NH 4 + addition stimulated AOB growth, and the growth could be significantly inhibited by acetylene or 1-octyne in the two soils. The stimulation of N 2 O emissions by NH 4 + and the relative suppression by inhibitors paralleled fluctuations in the AOB growth. In addition, cumulative N 2 O emissions were not correlated with AOA abundance in the two soils. Our results revealed that AOB could contribute more to soil N 2 O production than AOA in the NH 4 + -amended arable soils.

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Long‐Term Field Fertilization Significantly Alters Community Structure of Ammonia‐Oxidizing Bacteria rather than Archaea in a Paddy Soil

Cited by 124 publications

References 45 publications

Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil

Effect of chemical fertilization and green manure on the abundance and community structure of ammonia oxidizers in a paddy soil

Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Nitrogen fertiliser-induced changes in N2O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

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