Long-term follow-up of a Japanese Patient With Hepatitis B Virus Genotype H and Human Immunodeficiency Virus Coinfection

Ogawa, Masahiro; Kamimura, Shinya; Takahashi, Hiroshi; Mizutani, Taku; Matsumoto, Naoki; Yamamoto, Takeshi; Nirei, Kazushige; Matsuoka, Satoshi; Takei, Masami; Moriyama, Mitsuhiko

doi:10.2217/fvl-2019-0089

Cited by 2 publications

(2 citation statements)

References 30 publications

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“…GT-Ae, the original European genotype A; GT-Aa, the new African/Asian genotype A. The accession numbers of sequences of various HBV genotypes (GTs) are indicated [ 21 , 22 , 23 , 27 , 28 ].…”

Section: Figurementioning

confidence: 99%

Analysis of HBV Genomes Integrated into the Genomes of Human Hepatoma PLC/PRF/5 Cells by HBV Sequence Capture-Based Next-Generation Sequencing

Ishii

Tamura

Shibata

et al. 2020

Genes

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Hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma (HCC) worldwide. The integration of HBV genomic DNA into the host genome occurs randomly, early after infection, and is associated with hepatocarcinogenesis in HBV-infected patients. Therefore, it is important to analyze HBV genome integration. We analyzed HBV genome integration in human hepatoma PLC/PRF/5 cells by HBV sequence capture-based next-generation sequencing (NGS) methods. We confirmed the results by using Sanger sequencing methods. We observed that HBV genotype A is integrated into the genome of PLC/PRF/5 cells. HBV sequence capture-based NGS is useful for the analysis of HBV genome integrants and their locations in the human genome. Among the HBV genome integrants, we performed functional analysis and demonstrated the automatic expression of some HBV proteins encoded by HBV integrants from chromosomes 3 and 11 in Huh7 cells transfected with these DNA sequences. HBV sequence capture-based NGS may be a useful tool for the assessment of HBV genome integration into the human genome in clinical samples and suggests new strategies for hepatocarcinogenesis in HBV infection.

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Section: Figurementioning

confidence: 99%

Analysis of HBV Genomes Integrated into the Genomes of Human Hepatoma PLC/PRF/5 Cells by HBV Sequence Capture-Based Next-Generation Sequencing

Ishii

Tamura

Shibata

et al. 2020

Genes

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“…The primers used for PCR were 5 -GGTTTTTCACCTCTGCCTARTCATCTCWTGTWCATGT-3 (HBVfull P1C: sense, nt 1821-1855) and 5 -GGAAAAAGTTGCATGGTGCTGGTGMRCAGACCAATTT-3 (HBVfull-P2C: antisense, nt 1826-1791) [11]. These two sets of amplification primers were made at the position of the HBV X region based on the sequences of HBV GT-C (AB014376) [12].…”

Section: Extraction Of Serum Dna and Amplification Of Hbv Dna By Polymerase Chain Reactionmentioning

confidence: 99%

Analysis of full-length Hepatitis B Virus Genome From Chronic Hepatitis B-patients With Higher Alanine Aminotransferase Elevation

et al. 2020

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Background & aim: Higher elevation of alanine aminotransferase (ALT) occasionally leads to severe outcomes in hepatitis B virus (HBV)-infected patients. Our aim is to investigate the HBV sequence mutations associated with higher ALT elevation. Materials & methods: We analyzed full-length HBV sequences from patients with or without higher ALT elevation. Results: Nucleotide mutations in precore and core regions, which are associated with severe hepatitis B, were found in two HBV-infected patients with higher ALT elevation. Amino acid mutations within the pre-S1, pre-S2 and S regions were also found in a patient with HBV virologic breakthrough during the use of nucleoside analogs. Conclusion: It may be useful for HBV-infected patients with higher ALT elevation to analyze full-length HBV genome.

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Long-term follow-up of a Japanese Patient With Hepatitis B Virus Genotype H and Human Immunodeficiency Virus Coinfection

Cited by 2 publications

References 30 publications

Analysis of HBV Genomes Integrated into the Genomes of Human Hepatoma PLC/PRF/5 Cells by HBV Sequence Capture-Based Next-Generation Sequencing

Analysis of HBV Genomes Integrated into the Genomes of Human Hepatoma PLC/PRF/5 Cells by HBV Sequence Capture-Based Next-Generation Sequencing

Analysis of full-length Hepatitis B Virus Genome From Chronic Hepatitis B-patients With Higher Alanine Aminotransferase Elevation

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