Long-term, functional culture andin vitromanipulation of adult mouse cardiomyocytes

Abstract

word count: 57 Main text word count (with abstract): 1507 Running title: Long-term culture of adult cardiomyocytes Long-term culture of adult cardiomyocytes Callaghan et al. 2 Abstract 1Primary adult cardiomyocyte (aCM) culture is challenged by poor survival and loss of phenotype, 2 rendering extended in vitro experiments unfeasible. Here, we establish murine aCM culture methods that 3 enhance survival and maintain sarcomeric structure and Ca 2+ cycling to enable physiologically-relevant 4 contractile force measurements. We also demonstrate genetic and small-molecule manipulations that 5 probe mechanisms underlying myocyte functional performance. 6