Long term <i>in vitro</i>‐cultured plant cells show typical neoplastic features at the cytological level

Häsler, Julien; Wüest, Jean; Gaspar, Thomas; Crèvecœur, Michèle

doi:10.1016/s0248-4900(03)00077-7

Cited by 22 publications

(21 citation statements)

References 17 publications

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“…2d). The functions of these protuberances are unknown, but some reports suggest these protuberances sometimes contain an accessory nucleus, which is related to cell division (Häsler et al 2003).…”

Section: Fatty Acid Analysesmentioning

confidence: 99%

Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls

Guo

et al. 2009

Plant Cell Tiss Organ Cult

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Callus browning is a typical feature of callus cultures derived from the hypocotyl of Jatropha curcas. Brown callus results in decreased regenerative ability, poor growth and even death. In this study, we investigated the effect of browning on callus morphology and biochemical indices. Light microscopy and scanning electron microscopy showed striking differences in callus morphology. During browning, chlorophylls and carotenoids concentrations decreased steadily. Polyphenol oxidase (PPO) and peroxidase (POD) enzymatic activities patterns were similar during callus culture with a higher activity level at week 3 compared to week 2 or later weeks. Grey relation degree analysis indicated that PPO played a more important role than POD in enzymatic callus browning. Polyacrylamide gel electrophoresis results showed differences between browning and non-browning callus. Gas chromatographymass spectrometry results showed that saturated and unsaturated fatty acid quantities differed significantly but there was little difference in fatty acid composition between non-browning and browning callus. Differences in 17, 18.4 and 25 kDa protein concentrations were also observed in browning and non-browning callus using sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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Section: Fatty Acid Analysesmentioning

confidence: 99%

Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls

Guo

et al. 2009

Plant Cell Tiss Organ Cult

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“…The functions of protuberances are still unknown. HÄSLER et al (2003) suggested that these buds sometimes contain an accessory nucleus, which is related to cell division.…”

Section: Morphology Analysis Of Transgene Callimentioning

confidence: 99%

Agrobacterium tumefaciens-mediated Transformation of Jatropha curcas: Factors Affecting Transient Transformation Efficiency and Morphology Analysis of Transgenic Calli

Pasapula

et al. 2009

Silvae Genetica

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An efficient system for Agrobacterium tumefaciensmediated transformation of Jatropha curcas was developed in this study. Several factors affecting the transformation efficiency were optimized, including the explant type, preculture and coculture periods, usage of acetosyringone and density of A. tumefaciens. Compared with other explants, 2-day precultured and cocultured hypocotyl explants showed a significant GUS transient expression efficiency (67.7 %). Moreover, adding AS showed a remarkable increase in transformation efficiency. After infecting with A. tumefaciens, hypocotyl explants were subjected to expansion and proliferation on MS medium with 1.0 mg · l -1 IBA and 0.5 mg · l -1 BA. Transformants were demonstrated by the GUS assay and PCR analysis. Rate of hypocotyl explants forming resistant calli reached 67.08 %. Furthermore, in the transformed calli development, morphologic changes (calli superficial development and internal structure) were observed by SEM and LM. The present study has provided a fundamental information and research approach for the future study of inducing new traits to J. curcas and genetic modification.

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“…Minor discrepancies between these previous results and the present work are probably due to variations of the in vitro cultures during the intervening years (Hasler et al 2003), as well as to the methods used (spectrophotometric analysis vs. HPLC determination). However, our Wrst aim was to monitor the basic cell redox state of these lines.…”

Section: Cell Redox and Cell Diverentiation Statesmentioning

confidence: 99%

Relationship between DNA methylation and histone acetylation levels, cell redox and cell differentiation states in sugarbeet lines

Causevic

Gentil

Delaunay

et al. 2006

Planta

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In order to evaluate the permanent chromatin remodeling in plant allowing their high developmental plasticity, three sugarbeet cell lines (Beta vulgaris L. altissima) originating from the same mother plant and exhibiting graduate states of differentiation were analyzed. Cell differentiation has been estimated by the cell redox state characterized by 36 biochemical parameters as reactive oxygen species steady-state levels, peroxidation product contents and enzymatic or non-enzymatic protective systems. Chromatin remodeling has been estimated by the measurement of levels of DNA methylation, histone acetylation and corresponding enzyme activities that were shown to differ between cell lines. Furthermore, distinct loci related to proteins involved in cell cycle, gene expression regulation and cell redox state were shown by restriction landmark genome scanning or bisulfite sequencing to display differential methylation states in relation to the morphogenic capacity of the lines. DNA methylating, demethylating and/or histone acetylating treatments allowed to generate a collection of sugarbeet cell lines differing by their phenotypes (from organogenic to dedifferentiated), methylcytosine percentages (from 15.0 to 43.5%) and acetylated histone ratios (from 0.37 to 0.52). Correlations between methylcytosine or acetylated histone contents and levels of various parameters (23 or 7, respectively, out of 36) of the cell redox state could be established. These data lead to the identification of biomarkers of sugarbeet morphogenesis in vitro under epigenetic regulation and provide evidence for a connection between plant morphogenesis in vitro, cell redox state and epigenetic mechanisms.

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Long term in vitro‐cultured plant cells show typical neoplastic features at the cytological level

Cited by 22 publications

References 17 publications

Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls

Changes in morphology and biochemical indices in browning callus derived from Jatropha curcas hypocotyls

Agrobacterium tumefaciens-mediated Transformation of Jatropha curcas: Factors Affecting Transient Transformation Efficiency and Morphology Analysis of Transgenic Calli

Relationship between DNA methylation and histone acetylation levels, cell redox and cell differentiation states in sugarbeet lines

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