Long‐term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor

Kuzin, Igor; Sun, Hongliang; Moshkani, Safiekhatoon; Feng, Changyong; Mantalaris, Athanasios; Wu, Junchen; Bottaro, Andrea

doi:10.1002/bit.23055

Cited by 19 publications

(12 citation statements)

References 40 publications

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“…LECs can also attach to unwoven polyglycolic acid scaffolds [246]. In order to simulate lymph nodes, nonwoven polyamides, agarose matrix sheets, and macroporous cellulose microcarriers within an in vitro bioreactor have been utilized [247, 248].…”

Section: Techniques For Lymphatic Tissue Engineeringmentioning

confidence: 99%

Lymphatic Tissue Engineering and Regeneration

2018

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The lymphatic system is a major circulatory system within the body, responsible for the transport of interstitial fluid, waste products, immune cells, and proteins. Compared to other physiological systems, the molecular mechanisms and underlying disease pathology largely remain to be understood which has hindered advancements in therapeutic options for lymphatic disorders. Dysfunction of the lymphatic system is associated with a wide range of disease phenotypes and has also been speculated as a route to rescue healthy phenotypes in areas including cardiovascular disease, metabolic syndrome, and neurological conditions. This review will discuss lymphatic system functions and structure, cell sources for regenerating lymphatic vessels, current approaches for engineering lymphatic vessels, and specific therapeutic areas that would benefit from advances in lymphatic tissue engineering and regeneration.

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Section: Techniques For Lymphatic Tissue Engineeringmentioning

confidence: 99%

Lymphatic Tissue Engineering and Regeneration

2018

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“…In terms of an in vitro attempt to create the human LN environment, it was demonstrated that antigen-specific B cell clusters could be observed in a bioreactor -an ex vivo culture device -containing a porous matrix with antigen-primed DCs (Giese et al 2006). Long-term human peripheral lymphoid tissue cultures with immunologically competence in a 3D bioreactor have been reported (Kuzin et al 2011). Potentially useful cell lines of artificial antigen-presenting cells (aAPC) were established for humans (Butler et al 2007).…”

Section: Attempts To Generate Human Peripheral Lymphoid Tissuesmentioning

confidence: 99%

Synthesis of Functional Tertiary Lymphoid Organs

Kobayashi

Kato

Nakamura

et al. 2016

Synthetic Immunology

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The regeneration of functional immune organ will be one of major focus in future immunology research. It will be an useful tool which induces efficient immune responses in the body on demand and offers effective ways to restore the immune status and treat uncontrollable obstinate diseases such as cancer, autoimmune diseases, severe infection and immuno-insufficiency/deficiency caused by tissue damages, abnormality, primary defect and aging. Artificially synthesized lymphoid organs may also provide us with a highly informative method not only for clinical aim but also basic study on the development and functions of immunological tissues and organs. We first reported successful generation of artificiallyconstructed lymph node-like tertiary lymphoid tissues at ectopic sites in mouse by applying certain stromal cell lines (Suematsu S, Watanabe T. Nat Biotechnol 22 (11):422-428, 2010). They showed a remarkable ability to induce immune responses upon antigen stimulation, especially when transplanted into naïve or immune-compromised hosts. In this review, we discuss about the rationale and method for the synthesis of functional tertiary lymph node-like lymphoid tissues in mouse. Especially, we discuss here on the method with applying only soluble factors but without using any stromal cell, that enables proper accumulation and functional organization of immune cells in grafts.

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“…Both of these approaches are being extended to immune organs – LNs, thymus, and BM – to create tools to elucidate immune cell interactions and the link to immune response and disease. This work has already identified matrix porosity, fluid flow, cytokine expression, and stromal cell interactions as important design parameters 17, 18, 19, 20, 21 .…”

Section: Immune Organoids and Organs-on-a-chipmentioning

confidence: 99%

Designing natural and synthetic immune tissues

et al. 2018

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Vaccines and immunotherapies have provided enormous improvements for public health, but there are fundamental disconnects between where most studies are performed-in cell culture and animal models-and the ultimate application in humans. Engineering immune tissues and organs, such as bone marrow, thymus, lymph nodes and spleen, could be instrumental in overcoming these hurdles. Fundamentally, designed immune tissues could serve as in vitro tools to more accurately study human immune function and disease, while immune tissues engineered for implantation as next-generation vaccines or immunotherapies could enable direct, on-demand control over generation and regulation of immune function. In this Review, we discuss recent interdisciplinary strategies that are merging materials science and immunology to create engineered immune tissues in vitro and in vivo. We also highlight the hurdles facing these approaches and the need for comparison to existing clinical options, relevant animal models, and other emerging technologies.

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Long‐term immunologically competent human peripheral lymphoid tissue cultures in a 3D bioreactor

Cited by 19 publications

References 40 publications

Lymphatic Tissue Engineering and Regeneration

Lymphatic Tissue Engineering and Regeneration

Synthesis of Functional Tertiary Lymphoid Organs

Designing natural and synthetic immune tissues

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