Long-Term Intake of a High-Protein Diet with or without Potassium Citrate Modulates Acid-Base Metabolism, but Not Bone Status, in Male Rats

Mardon, Julie; Habauzit, Vèronique; Trzeciakiewicz, Anna; Davicco, Marie-Jeanne; Lebecque, Patrice; Mercier, Sylvie; Tressol, J.C.; Horcajada, Marie‐Noëlle; Demigné, Christian; Coxam, Véronique

doi:10.1093/jn/138.4.718

Cited by 33 publications

(32 citation statements)

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“…These results are consistent with previous observations in rats (16,21) that reported no change in femoral bone mass, macro-mineral composition, or urinary excretion of pyridinium crosslinks of collagen in response to a dietary protein excess, despite hypercalciuria. Zernicke et al (22) found a greater femoral neck percent ash in 8-wk-old rats fed a 30% protein diet (versus 15%), however, without any improvement regarding structural and material properties.…”

Section: Discussionsupporting

confidence: 93%

“…Calcium and magnesium concentrations in food, urine, and faecal samples were determined by atomic absorption spectrophotometry (AA800, Perkin Elmer, Norwalk, CT), as described previously (16). Then, calcium retention (mol/24 h), defined as [calcium intake Ϫ (urinary calcium excretion ϩ faecal calcium excretion)] was calculated.…”

Section: Methodsmentioning

confidence: 99%

“…Phosphate, sulfate, and citrate concentrations were assayed by ionic chromatography (DX320, Dionex, Sunnydale, CA) (16).…”

Section: Methodsmentioning

confidence: 99%

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Dietary Protein Supplementation Increases Peak Bone Mass Acquisition in Energy-Restricted Growing Rats

et al. 2009

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Peak bone mass is a major determinant of osteoporosis pathogenesis during aging. Respective influences of energy and protein supplies on skeletal growth remains unclear. We investigated the effect of a 5-mo dietary restriction on bone status in young rats randomized into six groups (n ϭ 10 per group). Control animals were fed a diet containing a normal (13%) (C-NP) or a high-protein content (26%) (C-HP). The other groups received a 40% protein energy-restricted diet (PER-NP and PER-HP) or a 40% energyrestricted diet (ER-NP and ER-HP). High-protein intake did not modulate bone acquisition, although a metabolic acidosis was induced and calcium retention impaired. PER and ER diets were associated with a decrease in femoral bone mineral density. The compensation for protein intake in energy-restricted conditions induced a bone sparing effect. Plasma osteocalcin (OC) and urinary deoxypyridinoline (DPD) assays revealed a decreased OC/DPD ratio in restricted rats compared with C animals, which was far more reduced in PER than in ER groups. Circulating IGF-1 levels were lowered by dietary restrictions. In conclusion, both energy and protein deficiencies may contribute to impairment in peak bone mass acquisition, which may affect skeleton strength and potentially render individuals more susceptible to osteoporosis. In particular, alterations in energy intake have been demonstrated to impair bone quality and strength in rodent models (1-5). Lamothe et al. (1) and Mardon et al. (2) reported that energy restriction adversely affected bone mineral content (BMC), bone mineral density (BMD), and mechanical properties in old rats, despite a micronutrient compensation. Nevertheless, the impact of energy restriction on bone was suggested to be modulated by the age at onset of restriction (5,6). To date, no clear consensus regarding the effects of energy restriction on peak bone mass acquisition has been established.Protein intake is widely linked to bone growth, hence to bone strength (7). Indeed, collagen is the major constituent of bone organic matrix, and noncollagenic proteins are involved in the regulation of the mineralization process. Dietary proteins provide the essential amino acids necessary for new matrix synthesis and may modulate circulating IGF-1 levels as well, an osteotrophic growth factor (8). In growing animals, protein deficiency was demonstrated to lead to decreased bone status (9). Ammann et al. (10) also reported that a 2.5% casein diet during 16 wk in 5-mo-old rats was associated with bone loss.Consequently, one would expect high-protein intake to promote bone health. However, other reports suggest that such a diet may alter calcium homeostasis in ways that could lead to bone loss (11,12). The metabolic acidosis, derived from sulfur amino acids catabolism, would also exert a direct stimulatory effect on bone resorption and an inhibitory action on matrix mineralization (13,14). Actually, the role of dietary protein on bone remains controversial.In the current context of rising incidence of obesity...

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

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Dietary Protein Supplementation Increases Peak Bone Mass Acquisition in Energy-Restricted Growing Rats

et al. 2009

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“…Thus, the need of alkaline organic salts increased to neutralize the endogenous acid production associated with excessive protein intake. And the supplementation of potassium citrate in the diet for male rat neutralized the metabolic acidosis induced by high dietary protein intake (Mardon et al, 2008). The lower potassium requirement in our study seems to correlate to the lower dietary protein content, however, direct proofs should be further investigated.…”

Section: Discussionmentioning

confidence: 57%

“…There are some evidences suggesting that dietary protein level would influence the potassium requirement. Longterm higher dietary protein induced metabolic acidosis in male rat, which was neutralized by the supplementation of potassium citrate (Mardon et al, 2008). Li et al (2011) also reported that the gill Na + -K + ATPase mRNA expression increased with the dietary protein level in L. vannamei.…”

Section: Introductionmentioning

confidence: 96%

Quantifying the dietary potassium requirement of juvenile grass carp (Ctenopharyngodon idellus)

Zhu¹,

Liu²,

Chen³

et al. 2014

Aquaculture

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A growth trial lasting for 12 weeks was conducted in a semi flow-through system to determine the dietary potassium requirement of juvenile grass carp (Ctenopharyngodon idellus) with an initial body weight of 4.8 g. Seven isonitrogenous and isoenergetic semi-purified diets were compounded with different dietary potassium levels: 1. 50, 2.32, 3.28, 5.30, 7.12, 9.45 and 10.44 g kg −1 diet. The results showed that specific growth rate and feed efficiency of grass carp were significantly (P b 0.05) affected by dietary potassium level, the highest value of specific growth rate was in 5.30 g kg −1 group and feed efficiency values in 5.30, 7.12 and 9.45 g kg −1 groups were significantly higher than those in the other groups. The survival rate of grass carp in the present study was not affected by dietary potassium level (P N 0.05). The Na + -K + ATPase activity value in the gills varied significantly (P b 0.05) with dietary potassium level and showed an increasing and then decreasing trend, and the highest value was in the 7.12 g kg −1 group. Dietary potassium level showed significant (P b 0.05) effect on serum SOD, ALP and AST activities. SOD and ALP activities increased and then decreased as potassium level increased, while AST decreased first and then increased. Serum TP and TC contents increased and then decreased with potassium level (P b 0.05). TG, ALT and BUN were not affected by dietary potassium level (P N 0.05). Body potassium and moisture contents increased and lipid content decreased with dietary potassium content, while ash content in fish body increased first and then decreased as potassium content increased (P b 0.05). Body protein in the 10.44 g kg −1 group was significantly lowered (P b 0.05). However, calcium, magnesium, phosphorus and sodium contents in dry matter were not affected by dietary potassium level. When the potassium content of water was 1.86-3.10 mg L −1 , judging from the polynomial regression analysis for the specific growth rate of fish and the Na + -K + ATPase activity in the gill, and the broken-line analysis for body potassium content, the optimal dietary potassium content for juvenile grass carp was 4.65, 7.27 and 5.98 g kg −1 diet respectively.

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Orange and Grapefruit Bioactive Compounds, Health Benefits and Other Attributes

Cancalon

2013

Bioactives in Fruit

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Long-Term Intake of a High-Protein Diet with or without Potassium Citrate Modulates Acid-Base Metabolism, but Not Bone Status, in Male Rats

Cited by 33 publications

References 52 publications

Dietary Protein Supplementation Increases Peak Bone Mass Acquisition in Energy-Restricted Growing Rats

Dietary Protein Supplementation Increases Peak Bone Mass Acquisition in Energy-Restricted Growing Rats

Quantifying the dietary potassium requirement of juvenile grass carp (Ctenopharyngodon idellus)

Orange and Grapefruit Bioactive Compounds, Health Benefits and Other Attributes

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