2020
DOI: 10.1038/s41598-020-61325-3
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Long-term Intracellular Recording of Optogenetically-induced Electrical Activities using Vertical Nanowire Multi Electrode Array

Abstract: Continuous recording of intracellular activities in single cells is required for deciphering rare, dynamic and heterogeneous cell responses, which are missed by population or brief single-cell recording. Even if the field of intracellular recording is constantly proceeding, several technical challenges are still remained to conquer this important approach. Here, we demonstrate long-term intracellular recording by combining a vertical nanowire multi electrode array (VNMEA) with optogenetic stimulation to minima… Show more

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Cited by 29 publications
(32 citation statements)
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“…The realization that the use of substrate integrated planar MEA technologies for extracellular recordings (Figure 1) inherently limits the qualities of in-vitro and in-vivo systems has prompted the development of new 3D in-vitro technologies to enable parallel, multisite intracellular recordings and stimulation from many individual cultured cells (neurons, cardiomyocytes and striated muscles). In principle, this family of in-vitro MEA technologies utilizes different forms of 3D vertical nano-structures (nano-pillars) that pierce the plasma membrane of cultured cells (by electroporation or spontaneously) in a way similar to classical sharp electrodes (Figure 1 and Tian et al, 2010;Angle and Schaefer, 2012;Duan et al, 2012;Gao et al, 2012;Robinson et al, 2012;Angle et al, 2014;Qing et al, 2014;Abbott et al, 2017;Dipalo et al, 2017;Liu et al, 2017;Abbott et al, 2018;Abbott et al, 2019;Mateus et al, 2019;Li et al, 2020;Teixeira et al, 2020;Yoo et al, 2020;Xu et al, 2021;Zhang et al, 2021). At the same time, a number of laboratories have developed the "IN-CELL" recording and stimulation configuration, in which micrometer-sized, extracellular gold mushroom-shaped microelectrodes (gMμEs) record attenuated synaptic and action potentials (Figure 1 and Spira et al, 2007;Hai et al, 2010b;a;Fendyur and Spira, 2012;Spira and Hai, 2013;Rabieh et al, 2016;Shmoel et al, 2016;Weidlich et al, 2017;McGuire et al, 2018;Spira et al, 2018;Mateus et al, 2019;Spira et al, 2019;…”
Section: Introductionmentioning
confidence: 99%
“…The realization that the use of substrate integrated planar MEA technologies for extracellular recordings (Figure 1) inherently limits the qualities of in-vitro and in-vivo systems has prompted the development of new 3D in-vitro technologies to enable parallel, multisite intracellular recordings and stimulation from many individual cultured cells (neurons, cardiomyocytes and striated muscles). In principle, this family of in-vitro MEA technologies utilizes different forms of 3D vertical nano-structures (nano-pillars) that pierce the plasma membrane of cultured cells (by electroporation or spontaneously) in a way similar to classical sharp electrodes (Figure 1 and Tian et al, 2010;Angle and Schaefer, 2012;Duan et al, 2012;Gao et al, 2012;Robinson et al, 2012;Angle et al, 2014;Qing et al, 2014;Abbott et al, 2017;Dipalo et al, 2017;Liu et al, 2017;Abbott et al, 2018;Abbott et al, 2019;Mateus et al, 2019;Li et al, 2020;Teixeira et al, 2020;Yoo et al, 2020;Xu et al, 2021;Zhang et al, 2021). At the same time, a number of laboratories have developed the "IN-CELL" recording and stimulation configuration, in which micrometer-sized, extracellular gold mushroom-shaped microelectrodes (gMμEs) record attenuated synaptic and action potentials (Figure 1 and Spira et al, 2007;Hai et al, 2010b;a;Fendyur and Spira, 2012;Spira and Hai, 2013;Rabieh et al, 2016;Shmoel et al, 2016;Weidlich et al, 2017;McGuire et al, 2018;Spira et al, 2018;Mateus et al, 2019;Spira et al, 2019;…”
Section: Introductionmentioning
confidence: 99%
“…A limitation of the system is the inability to detect subthreshold events due to the extracellular nature of the multielectrode array recording, and thus to investigate the potential relationship between miniature excitatory and inhibitory postsynaptic currents and connectivity. Recently, the application of nanotechnology on neuronal electrophysiology has brought about a promising solution to overcome this limitation and to fabricate devices that are capable of detecting sub-threshold potential (Liu et al, 2017;Wei et al, 2018;Yoo et al, 2020). Although substantial engineering issues remain before the potential of nano-neural interfaces can be fully exploited (Wu et al, 2020), the future application of our algorithm to more sensitive recordings appear to be a promising approach for a more accurate understanding of network connectivity as a consequence of synaptic and extra-synaptic inputs as well as subthreshold potentials in both physiological and pathological conditions.…”
Section: Discussionmentioning
confidence: 99%
“…Adapted with permission. [ 81 ] Copyright 2020, Springer Nature. Neuronal studies: c) vertical nanowire multielectrode array.…”
Section: Recent Advances: Novel Recording‐stimulation Architecturesmentioning
confidence: 99%
“…Vertical‐type electrodes with high aspect ratio have been used as probes for large cells which have higher coupling probability and consequently form a tight seal with the electrode. [ 21,81,96,98 ] In contrast, neuronal cells have a much smaller body size reducing the probability of active coupling electrode sites, hindering the study of neuronal connections which is still a problem for GMμEAs. [ 7 ] Nevertheless, Kwon et al.…”
Section: Recent Advances: Novel Recording‐stimulation Architecturesmentioning
confidence: 99%