Long-term live cell microscopy studies of lipid droplet fusion dynamics in adipocytes

Jungreuthmayer, Christian; Klein, Matthias; Zumbusch, Andreas

doi:10.1194/jlr.m042515

Cited by 48 publications

(37 citation statements)

References 64 publications

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“…This process is thought to be distinct from much faster LD coalescence events which have been observed in vitro and the in vivo relevance of which are still debated; current consensus suggests that coalescence probably does not occur in vivo, although depleting surface PC or the addition of certain pharmacological agents can destabilise LDs and promote this type of rapid fusion [47,48]. LD permeation has also been observed using CARS imaging in differentiating adipocytes, where it was possible to precisely document the additive effect of several 'permeation' events on LD volume and measure factors influencing the rate of lipid transfer between LDs [49,50 ]. The latter suggests that this depends primarily on the volume of the smaller LD.…”

Section: Ld-ld Contact Sitesmentioning

confidence: 96%

Lipid droplet–organelle interactions: emerging roles in lipid metabolism

Barbosa

Savage

Siniossoglou

2015

Current Opinion in Cell Biology

146

106

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Cellular homeostasis depends on the precisely coordinated use of lipids as fuels for energy production, building blocks for membrane biogenesis or chemical signals for intra-cellular and inter-cellular communication. Lipid droplets (LDs) are universally conserved dynamic organelles that can store and mobilize fatty acids and other lipid species for their multiple cellular roles. Increasing evidence suggests that contact zones between LDs and other organelles play important roles in the trafficking of lipids and in the regulation of lipid metabolism. Here we review recent advances regarding the nature and functional relevance of interactions between LDs and other organelles-particularly the endoplasmic reticulum (ER), LDs, mitochondria and vacuoles-that highlight their importance for lipid metabolism.

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Section: Ld-ld Contact Sitesmentioning

confidence: 96%

Lipid droplet–organelle interactions: emerging roles in lipid metabolism

Barbosa

Savage

Siniossoglou

2015

Current Opinion in Cell Biology

146

106

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“…One example for such an experiment is the unraveling of the growth dynamics of lipid droplets (LDs) in live adipocytes as shown in Figure (Jüngst et al . ). LDs have long been known as the storage site for lipids in animal and plant cells.…”

Section: Applications Of Nonlinear Vibrational Microscopymentioning

confidence: 97%

“…This and the fact that no photobleaching occurs, allows longterm observation of lipids in living cells and model organisms (Jüngst et al, 2011;Zumbusch et al, 2013). One example for such an experiment is the unraveling of the growth dynamics of lipid droplets (LDs) in live adipocytes as shown in Figure 2 (Jüngst et al 2013). LDs have long been known as the storage site for lipids in animal and plant cells.…”

Section: Applications Of Nonlinear Vibrational Microscopymentioning

confidence: 99%

Nonlinear optical microscopy with vibrational contrast

Winterhalder

Zumbusch

2014

Journal of Microscopy

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Summary Nonlinear optical techniques which provide vibrational contrast have gained increasing attention in microscopy during the last two decades. After outlining the potential of these techniques, we give a brief introduction to coherent anti‐Stokes Raman scattering, stimulated Raman scattering and sum frequency generation and discuss their suitability for contrast generation in optical microscopy. The rapid developments in these fields during the last decade have resulted in many different applications. Three exemplary application areas will therefore be presented in the last part of this manuscript.

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“…Here, an especially interesting point has been to understand the growth mechanism of LDs in human adipocytes, which was largely unknown until recently. Long-term CARS microscopy experiments have shown that LDs indeed grow by fusion of smaller LDs [70,71]. Due to the slow fusion dynamics, fluorescence microscopy could not reveal these processes as the long observation times necessary ultimately lead to strong photobleaching of the fluorescent stains.…”

Section: Lipidsmentioning

confidence: 97%