Long‐term, long‐distance recording of a living migrating neuron by scanning ion conductance microscopy

Gesper, Astrid; Thatenhorst, Denis; Wiese, Stefan; Tsai, Teresa; Dietzel, Irmgard D.; Happel, Patrick

doi:10.1002/sca.21200

Cited by 9 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reason is that the imaging of AFM is realized by measuring the interaction force between probe and sample which will inevitably affect the cells. And the contactless imaging mechanism of SICM can avoid cell deformation 47,48 …”

Section: Application Of Sicm In Biomedical Researchmentioning

confidence: 99%

“…SICM can also be used to record living cells for a long time. Gesper et al used SICM to record the migration of neurons for a long time and long distance, indicating that SICM has little effect on cells which makes it have an irreplaceable position in scanning probe microscopy 47 …”

Section: Application Of Sicm In Biomedical Researchmentioning

confidence: 99%

“…And the contactless imaging mechanism of SICM can avoid cell deformation. 47,48 Gorelik et al are able to accurately detect the dynamics of microvilli through SICM. It is found that microvilli are membrane protrusions supported by actin bundles and go through three stages of life cycle of about 12 min.…”

Section: High-precision Imaging Of Cellular Structuresmentioning

confidence: 99%

See 2 more Smart Citations

Applications of scanning ion conductance microscope in biomedical fields

Gong

Fan

Huang

et al. 2023

MedComm – Biomaterials and Applications

View full text Add to dashboard Cite

Scanning ion conductance microscopy (SICM) is a scanning probe technique to reflect the surface morphology of the sample with the probe's motion trajectory through measuring the current between the probe and the surface of the sample. Since the surface of the sample can be scanned noncontact and the morphological characteristics of the living cell can be obtained under physiological conditions, SICM is particularly important in the field of biomedicine. SICM has strong potentials in various applications, such as the real-time and high-resolution imaging of living cells, monitoring the volume and movements of living cells. In addition, SICM combines with various technologies such as patch clamp and nanopipette to study the physical properties of cells. Furthermore, using SICM to observe cell and tissue structure provides more directions for studying the mechanism of disease. This review briefly introduces the principles and imaging modes of SICM and outlines the recent applications and development of SICM in biomedical research. We also introduce the limitations of the existing SICM technology and make a perspective on its future development.

show abstract

Section: Application Of Sicm In Biomedical Researchmentioning

confidence: 99%

Section: Application Of Sicm In Biomedical Researchmentioning

confidence: 99%

See 1 more Smart Citation

Applications of scanning ion conductance microscope in biomedical fields

Gong

Fan

Huang

et al. 2023

MedComm – Biomaterials and Applications

View full text Add to dashboard Cite

show abstract

“…Scanning ion conductance microscopy (SICM) was first proposed by Hansma et al in 1989, then further improved by Korchev et al , in 1997, and has become a formidable high-resolution microimaging tool. It has been applied to a wide range of applications in the fields of biology, medicine, and electrochemistry. − SICM uses a thin nanopipette filled with electrolyte solution as its scanning probe. By monitoring the change of ion current in the nanopipette, the distance between the nanopipette and sample can be controlled, which allows for reconstruction of the microscopic surface topography of the sample.…”

Section: Introductionmentioning

confidence: 99%

Study on a Rapid Imaging Method for Scanning Ion Conductance Microscopy Using a Double-Barreled Theta Pipette

et al. 2020

View full text Add to dashboard Cite

Scanning ion conductance microscopy (SICM) is a new noncontact, high-resolution scanning probe microscopy technique, which has become increasingly popular in recent years. The hopping modecurrently the most widely used scanning modecan be used for imaging samples with complicated surface topographies. However, its slow scanning rate seriously restricts its broader application. This paper proposes a fast imaging control mode using a doublebarreled theta pipette as the probe, which effectively increases the imaging rate. In this mode, sample surface height information is obtained when the doublebarreled theta pipette approaches the sample in a two-step downward process. The ion current sum of two barrels and ion current of one barrel are used as feedback signals to approach the sample until the feedback signals decrease to the set threshold, respectively, thereby obtaining the height of the imaging point. First, this work used COMSOL to establish an SICM model and perform simulation analysis. The simulation results verified the proposed method's feasibility. Second, a scanning time mathematical model was established. The results revealed that the new method is superior to the traditional method in terms of imaging rate. Finally, experiments were performed on poly(dimethylsiloxane) (PDMS) samples using the two imaging modes described above. The results demonstrated that the new scanning mode could significantly improve the imaging rate of SICM without a loss in imaging quality and stability.

show abstract

“…However, AFM has been found to bias cellular samples more than SICM . The nearly contact-free operating principle of SICM, operated in a mode where the scanning tip is advanced to the sample at every single pixel and subsequently withdrawn before lateral movement to avoid probe sample collisions − (see also Figure E), allowed SICM to, for example, determine the volume changes during the migration of neural cells. − Hitherto, no combined recordings of SICM and any fluorescence super-resolution technique have been reported, but direct stochastic optical reconstruction microscopy (dSTORM) has been used to quantify the number of fluorescent molecules deposited by a SICM tip onto a surface . However, SICM has been combined with conventional confocal fluorescence microscopy .…”

mentioning

confidence: 99%

Correlative Stimulated Emission Depletion and Scanning Ion Conductance Microscopy

2018

Self Cite

View full text Add to dashboard Cite

Correlation microscopy combining fluorescence and scanning probe or electron microscopy is limited to fixed samples due to the sample preparation and nonphysiological imaging conditions required by most probe or electron microscopy techniques. Among the few scanning probe techniques that allow imaging of living cells under physiological conditions, scanning ion conductance microscopy (SICM) has been shown to be the technique that minimizes the impact on the investigated sample. However, combinations of SICM and fluorescence microscopy suffered from the mismatch in resolution due to the limited resolution of conventional light microscopy. In the last years, the diffraction limit of light microscopy has been circumvented by various techniques, one of which is stimulated emission depletion (STED) microscopy. Here, we aimed at demonstrating the combination of STED and SICM. We show that both methods allow recording a living cellular specimen and provide a SICM and STED image of the same sample, which allowed us to correlate the membrane surface topography and the distribution of the cytoskeletal protein actin. Our proof-of-concept study exemplifies the benefit of correlating SICM with a subdiffraction fluorescence method and might form the basis for the development of a combined instrument that would allow the simultaneous recording of subdiffraction fluorescence and topography information.

show abstract

Long‐term, long‐distance recording of a living migrating neuron by scanning ion conductance microscopy

Cited by 9 publications

References 34 publications

Applications of scanning ion conductance microscope in biomedical fields

Applications of scanning ion conductance microscope in biomedical fields

Study on a Rapid Imaging Method for Scanning Ion Conductance Microscopy Using a Double-Barreled Theta Pipette

Correlative Stimulated Emission Depletion and Scanning Ion Conductance Microscopy

Contact Info

Product

Resources

About