Long-term maintenance of in vitro cultured honeybee (Apis mellifera) embryonic cells

Bergem, Monica; Norberg, Kari; Aamodt, Rolf

doi:10.1186/1471-213x-6-17

Cited by 34 publications

(7 citation statements)

References 51 publications

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“…Thus far, many attempts have resulted in several honey bee cell culture methods. These methods are highly varied as they use different target tissues, growth media and isolation methods (Bergem et al, 2006;Hunter, 2010;Ju and Ghil, 2015). Honey bee primary cell cultures have been established using different life stages from egg to adult bees and various isolated tissues including neural cells, antennae, fat body, hemocyte, and embryos (Kreissl and Bicker, 1992;Gascuel et al, 1994;Goldberg et al, 1999;Sorescu et al, 2003;Barbara et al, 2008;Ju and Ghil, 2015).…”

Section: Cell Line In Honey Beesmentioning

confidence: 99%

Bee Viruses: Routes of Infection in Hymenoptera

et al. 2020

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Numerous studies have recently reported on the discovery of bee viruses in different arthropod species and their possible transmission routes, vastly increasing our understanding of these viruses and their distribution. Here, we review the current literature on the recent advances in understanding the transmission of viruses, both on the presence of bee viruses in Apis and non-Apis bee species and on the discovery of previously unknown bee viruses. The natural transmission of bee viruses will be discussed among different bee species and other insects. Finally, the research potential of in vivo (host organisms) and in vitro (cell lines) serial passages of bee viruses is discussed, from the perspective of the host-virus landscape changes and potential transmission routes for emerging bee virus infections.

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Section: Cell Line In Honey Beesmentioning

confidence: 99%

Bee Viruses: Routes of Infection in Hymenoptera

et al. 2020

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“…This should be explored at both the individual and colony levels, since the information gained may guide the use of virus susceptibility as an additional selectable trait in honey bee breeding programs [129,132,133]. In addition, further use and development of immortalized honey bee lines (i.e., AmE-711) [134], long-term cell cultures [135], and primary cell cultures [136,137], are required to further the field of honey bee virology. Future use of immortalized cell lines and infectious honey bee virus clones will serve to normalize future studies and lead to a better understanding of honey bee antiviral defense mechanisms.…”

Section: Two Sides To the Story – Host Vs Virus Geneticsmentioning

confidence: 99%

Antiviral defense mechanisms in honey bees

Brutscher

Daughenbaugh

Flenniken

2015

Current Opinion in Insect Science

159

155

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Honey bees are significant pollinators of agricultural crops and other important plant species. High annual losses of honey bee colonies in North America and in some parts of Europe have profound ecological and economic implications. Colony losses have been attributed to multiple factors including RNA viruses, thus understanding bee antiviral defense mechanisms may result in the development of strategies that mitigate colony losses. Honey bee antiviral defense mechanisms include RNA-interference, pathogen-associated molecular pattern (PAMP) triggered signal transduction cascades, and reactive oxygen species generation. However, the relative importance of these and other pathways is largely uncharacterized. Herein we review the current understanding of honey bee antiviral defense mechanisms and suggest important avenues for future investigation.

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“…Currently, visualization tools and the genetic manipulation of honeybee embryos have been developed, including in situ hybridization [ 26 ], immunohistochemistry [ 27 ], RNA interference [ 28 , 29 ], transgenesis with the transposon piggyBac [ 30 ], and genome editing by the CRISPR/Cas9 method [ 31 ]. Short-term [ 32 ], long-term [ 33 ] and immortalized cell lines [ 34 ] have been successfully developed, and non- Apis genes can be expressed in cultured embryonic cells [ 35 ]. Meanwhile, high-throughput sequencing, also known as next-generation sequencing (NGS), has become essential for modern biological research, and it has been widely applied to honeybee genome [ 36 , 37 ], transcriptome [ 38 , 39 ] and metagenome [ 40 ] analyses.…”

Section: Introductionmentioning

confidence: 99%

Dynamic transcriptome landscape of Asian domestic honeybee (Apis cerana) embryonic development revealed by high-quality RNA sequencing

Wang

et al. 2018

BMC Dev Biol

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BackgroundHoneybee development consists of four stages: embryo, larva, pupa and adult. Embryogenesis, a key process of cell division and differentiation, takes 3 days in honeybees. However, the embryonic transcriptome and the dynamic regulation of embryonic transcription are still largely uncharacterized in honeybees, especially in the Asian honeybee (Apis cerana). Here, we employed high-quality RNA-seq to explore the transcriptome of Asian honeybee embryos at three ages, approximately 24, 48 and 72 h (referred to as Day1, Day2 and Day3, respectively).ResultsNine embryo samples, three from each age, were collected for RNA-seq. According to the staging scheme of honeybee embryos and the morphological features we observed, our Day1, Day2 and Day3 embryos likely corresponded to the late stage four, stage eight and stage ten development stages, respectively. Hierarchical clustering and principal component analysis showed that same-age samples were grouped together, and the Day2 samples had a closer relationship with the Day3 samples than the Day1 samples. Finally, a total of 18,284 genes harboring 55,646 transcripts were detected in the A. cerana embryos, of which 44.5% consisted of the core transcriptome shared by all three ages of embryos. A total of 4088 upregulated and 3046 downregulated genes were identified among the three embryo ages, of which 2010, 3177 and 1528 genes were upregulated and 2088, 2294 and 303 genes were downregulated from Day1 to Day2, from Day1 to Day3 and from Day2 to Day3, respectively. The downregulated genes were mostly involved in cellular, biosynthetic and metabolic processes, gene expression and protein localization, and macromolecule modification; the upregulated genes mainly participated in cell development and differentiation, tissue, organ and system development, and morphogenesis. Interestingly, several biological processes related to the response to and detection of light stimuli were enriched in the first-day A. cerana embryogenesis but not in the Apis mellifera embryogenesis, which was valuable for further investigations.ConclusionsOur transcriptomic data substantially expand the number of known transcribed elements in the A. cerana genome and provide a high-quality view of the transcriptome dynamics of A. cerana embryonic development.Electronic supplementary materialThe online version of this article (10.1186/s12861-018-0169-1) contains supplementary material, which is available to authorized users.

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Long-term maintenance of in vitro cultured honeybee (Apis mellifera) embryonic cells

Cited by 34 publications

References 51 publications

Bee Viruses: Routes of Infection in Hymenoptera

Bee Viruses: Routes of Infection in Hymenoptera

Antiviral defense mechanisms in honey bees

Dynamic transcriptome landscape of Asian domestic honeybee (Apis cerana) embryonic development revealed by high-quality RNA sequencing

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