Long‐term maintenance of lyophilized fungal cultures of the genera <i>Epidermophyton, Microsporum, Paecilomyces</i> and <i>Trichophyton</i>

Rybnikář, A.

doi:10.1111/j.1439-0507.1995.tb00038.x

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…Similar results with other hypha-forming fungi were found in previous studies (Banno et al 1979, Ellis and Roberson 1968, Haskins 1957, Haynes et al 1955, Smith and Onions 1983, 1994, Rybnikar 1985, 1995, Schipper and Bekker-Holtman 1976. Similar results with other hypha-forming fungi were found in previous studies (Banno et al 1979, Ellis and Roberson 1968, Haskins 1957, Haynes et al 1955, Smith and Onions 1983, 1994, Rybnikar 1985, 1995, Schipper and Bekker-Holtman 1976.…”

Section: Discussionsupporting

confidence: 90%

“…In one study, 151 different species of wood-inhabiting basidiomycetes were shown to have maintained their viability for 7 yr when stored in distilled water at 4 to 5 C (Burdsall and Dorworth 1994). Conidium-or spore-forming fungi preserve their original characteristics after lyophilization (Antheunisse 1973, Ellis and Roberson 1968, Haskins 1957, Haynes et al 1955, Rybnikar 1985, 1995, Schipper and Bekker-Holtman 1976, Smith 1984, Smith and Onions 1983, Tan et al 1991, 1994, Wickerham and Flickinger 1946. Conidium-or spore-forming fungi preserve their original characteristics after lyophilization (Antheunisse 1973, Ellis and Roberson 1968, Haskins 1957, Haynes et al 1955, Rybnikar 1985, 1995, Schipper and Bekker-Holtman 1976, Smith 1984, Smith and Onions 1983, Tan et al 1991, 1994, Wickerham and Flickinger 1946.…”

Section: Introductionmentioning

confidence: 99%

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Preservation of Tropical Wood-Inhabiting Basidiomycetes

1999

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.Abstract: The objective of this study was to develop a method to preserve tropical wood-inhabiting basidiomycetes in a viable state for an extended period. Storage in water, lyophilization, and cryopreservation methods of preserving tropical wood-inhabiting basiomycotina were compared. When tropical mycelial isolates were stored in sterile distilled water, 57% survived at 4 C for 2.5 mo; 92% of the isolates survived at 15 C for 10 mo. Bulbil-, clamydospore-, and conidium-forming fungi survived in cold water storage and maintained excellent viability for a decade. Vegetative hypha-, bulbil-, clamydospore-, and conidium-forming fungi survived cryopreservation using liquid nitrogen vapor. Only basidiomycetes that formed bulbils, chlamydospores, or conidia survived lyophilization. We conclude that the best method for long-term preservation of tropical fungi is cryopreservation by means of storage in liquid nitrogen vapor.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Preservation of Tropical Wood-Inhabiting Basidiomycetes

1999

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“…In the present study, total loss of viability for P. lilacinus varied from 17.9 to 37.5% after four years storage. Recently, Rybnikár (1995) reported losses in viability during storage ranging from 30.4 to 96.6% for 26 lyophilized cultures; for instance, the loss of viability for P. lilacinus was 56.1% after 11 years.…”

mentioning

confidence: 99%

“…With the exception of a few reports (Bunse & Steigleder, 1991;Rybnikár, 1995), in which the reduction in viability of lyophilized cultures was quantified by recording the number of Colony Forming Units (CFUs), previous publications dealing with the efficiency of long-term preservation of fungi considered only the capability of growth on synthetic media. According to this criterion, all strains used in the present study were viable following lyophilization, although the germination rates were extremely low in certain cases.…”

mentioning

confidence: 99%

Entomopathogenic fungal (Hyphomycetes) collection: assessment of conidial viability

Faria

Martins

Mello

et al. 1999

Pesq. agropec. bras.

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-Twenty four strains of the entomopathogenic fungi (Hyphomycetes) Beauveria bassiana, Metarrhizium anisopliae, Nomuraea rileyi, Paecilomyces farinosus, P. fumosoroseus and P. lilacinus, maintained in the culture collection of Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (Cenargen) and preserved by lyophilization and in liquid nitrogen, had their conidial viability assessed. Germination rates of 16-to 84-month-old cultures stored in liquid nitrogen decreased, on average, less than 13.3%. For 29-to 49-month-old cultures preserved by lyophilization, the viability loss ranged, on average, from 28.6 to 94.5%. The results demonstrated the efficiency of the tested methods, especially liquid nitrogen, in preserving the viability of entomopathogenic fungi. COLEÇÃO DE FUNGOS ENTOMOPATOGÊNICOS (HYPHOMYCETES):AVALIAÇÃO DA VIABILIDADE CONIDIAL RESUMO -Vinte e quatro isolados dos fungos entomopatogênicos (Hyphomycetes) Beauveria bassiana, Metarrhizium anisopliae, Nomuraea rileyi, Paecilomyces farinosus, P. fumosoroseus e P. lilacinus, mantidos na coleção de culturas da Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (Cenargen) e preservados em nitrogênio líquido e liofilização, tiveram a viabilidade conidial avaliada. As taxas de germinação de conídios preservados em nitrogê-nio líquido diminuíram, em média, menos de 13,3% após 16 a 84 meses de armazenamento. A perda de viabilidade de isolados liofilizados, após 29 a 49 meses de armazenamento, variou de 28,6 a 94,5%. Os resultados demonstraram a eficiência dos métodos testados, especialmente nitrogênio líquido, na preservação da viabilidade de fungos entomopatogênicos.In Brazil, the occurrence of over 20 genera of entomopathogenic fungi has been reported (Alves, 1992). Embrapa-Centro Nacional de Pesquisa de Recursos Genéticos e Biotecnologia (Cenargen), in Brasília, Brazil, is engaged in establishing an entomopathogenic fungal culture collection

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“…Freeze‐drying of fungi was reported on a large scale by Raper and Alexander 23 and the viability of their freeze‐dried cultures after storage for 23 years was subsequently reported by Ellis and Robertson 24 . Overdrying may kill in some cases and mutation of the DNA may occur in others 25 . A residual moisture content between 1 and 2% proved to be successful 14 .…”

Section: Resultsmentioning

confidence: 99%

The maintenance and preservation of keratinophilic fungi and related dermatophytes

Deshmukh

2003

Mycoses

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A total of 239 fungi belonging to nine genera and 23 species were maintained on agar slopes in a refrigerator at +8 +/- 1 degrees C and in sterile distilled water, under liquid paraffin, dried on silica gel, in sterile soil at room temperature. A limited number has been stored by freeze-drying between 1985 and 1998. It is recommended that each isolate should be maintained by at least two of these methods. The choice of the method will depend to a large extent on the equipment and facilities available in the laboratory. It is reported that simple methods can be used for preservation of keratinophilic fungi and related dermatophytes.

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Long‐term maintenance of lyophilized fungal cultures of the genera Epidermophyton, Microsporum, Paecilomyces and Trichophyton

Cited by 11 publications

References 18 publications

Preservation of Tropical Wood-Inhabiting Basidiomycetes

Preservation of Tropical Wood-Inhabiting Basidiomycetes

Entomopathogenic fungal (Hyphomycetes) collection: assessment of conidial viability

The maintenance and preservation of keratinophilic fungi and related dermatophytes

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