Long-term motility and fertility conservation of chilled canine semen using egg yolk added Tris–glucose extender: In vitro and in vivo studies

Verstegen, J.; Onclin, K.; Iguer-Ouada, Mokrane

doi:10.1016/j.theriogenology.2005.05.035

Cited by 63 publications

(66 citation statements)

References 15 publications

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“…Moreover, energy depletion may contribute to the negative consequences of prolonged storage time. These hypotheses were in agreement with Verstegen et al, who observed that cooled sperm stored for up to 96 h exhibits considerable recovery of progressive motility when the diluent was replaced daily [31].…”

Section: Discussionsupporting

confidence: 92%

The Efficacy of In Vitro Sperm Tests in Predicting Pregnancy Success after Artificial Insemination in the Bitch

Icn¹,

A²,

Cr³

et al. 2017

Andrology

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In many species, including the dog (Canis familiaris), semen analysis protocols has improved in recent years. In vitro assessment of semen quality provides important information; however, the ultimate parameter for determining semen quality is pregnancy outcome. The objective of the present study was to investigate the efficiency of in vitro sperm tests in predicting seminal quality in dogs by correlating those results with pregnancy success after artificial insemination (AI). Forty-eight semen samples refrigerated for 24, 48 or 72 hours were examined for sperm motility and progressive motility, membrane integrity, and zona pellucida-binding capacity before being used to artificially inseminate bitches. Results indicated positive correlations between pregnancy success and sperm motility (0.77), vigor (0.72), membrane integrity (0.72) and zona pellucida-binding (0.81). Both pregnancy success and the sperm characteristics declined with increased semen storage time. In conclusion, the semen characteristics evaluated in this study can be used to predict the conception potential of semen samples in the dog.

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Section: Discussionsupporting

confidence: 92%

The Efficacy of In Vitro Sperm Tests in Predicting Pregnancy Success after Artificial Insemination in the Bitch

Icn¹,

A²,

Cr³

et al. 2017

Andrology

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“…Martinez-Rodriguez et al (2012) afirman que el semen mantenido a 15ºC preserva su capacidad fertilizante hasta por 12 horas, sin embargo su motilidad puede permanecer estable por hasta 48 horas. Contrariando tales afirmaciones Verstegen et al (2005), en especies distintas, comprobaron que el cambio de medio puede elevar la motilidad espermática, del semen refrigerado por largos períodos. En el estudio aquí presentado, las cajas térmicas utilizadas son capaces de mantener la temperatura por máximo 48 horas, debido a la duración del hielo reciclable, lo que puede haber contribuido a la caída elevada de la motilidad después de este período.…”

Section: Resultados E Discusiónunclassified

Viabilidad de espermatozoides ovinos mantenidos a 5º y 15ºC en diferentes sistemas de refrigeración

Moscardini

Scott

Moura

et al. 2014

RBCV

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ResumenEl objetivo del presente estudio fue evaluar la cualidad espermática del semen refrigerado de carneros, en cajas térmicas con diferentes temperaturas. Fueron utilizados 6 eyaculados, de 2 carneros colectado con vagina artificial. En el semen se analizó volumen, movimiento en masa, motilidad, vigor, concentración espermática, test hipo-osmótico y coloración supra-vital. Las muestras fueron divididas en 2 alícuotas y diluidas en solución NaCl 0,9% o en un medio (Botubov ® , Botupharma, Botucatu, SP, Brasil); después, la dilución fue mantenida a temperatura ambiente, nevera (5ºC), caja Botubox ® (15ºC, Botupharma, Botucatu, SP, Brasil), caja Botutainer ® (5ºC, Botupharma, Botucatu, SP, Brasil) y caja MaxSemen ® (15ºC, EHG Agrofarma, Campinas, SP, Brasil). Todas las muestras fueron analizadas cada 24 horas, siguiendo los mismos parámetros. De acuerdo con los resultados, las muestras mantenidas en el diluyente presentaron viabilidad hasta 48 horas de refrigeración en las cajas térmicas y en la nevera, y la motilidad se mantuvo entorno de 30% hasta las 72 horas. Las muestras diluidas en solución NaCl 0,9% conservaron la motilidad en 30% hasta las 24 horas de refrigeración. Basado en los resultados se concluye que las tres cajas pueden ser utilizadas para transporte de semen ovino, diluido y refrigerado por un período de 24 a 48 horas.Palabras clave: carnero, crio-preservación, espermatozoide. AbstractThe aim of this study was to evaluate the ovine sperm quality colled in thermal boxs. Six ejaculates from 2 rams were collected using artificial vagina. Samples were analyzed to volume, mass movement, motility, vigor, sperm cell concentration, hypo-osmotic swelling test and supravital stain. Ejaculates were divided into 2 aliquots and diluted in 0.9% NaCl or in the extender (Botubov ® , Botupharma, Botucatu, SP, Brazil), and was kept at room temperature, refrigerator (5°C), Botubox ® (15°C, Botupharma, Botucatu, SP, Brazil), Botutainer ® (5ºC, Botupharma, Botucatu, SP, Brazil) and MaxSemen ® (15°C, EHG Agrofarma, Campinas, SP, Brazil). All samples were analyzed each 24 hours to the same parameters. According to the results the samples diluted and cooled in extender preserved sperm viability until 48 hours in thermal boxes and in refrigerator; and the samples extended preserved in environment maintained motility 30% up to 72 hours. Samples diluted in 0.9% saline retained motility around 30% until 24 hours of cooling. Based on the results it is concluded that the three boxes may be used for transport of ram semen, diluted and refrigerated for a period of 24 to 48 hours.Keywords: cryopreservation, ram, spermatozoa. IntroducciónLa criación de ovinos en los últimos años ha crecido y el mercado internacional de carne ovina se mantiene en alta, debido al menor costo de producción. Este crecimiento ha impulsado y estimulado la aplicación de biotécnicas de reproducción en la especie (Oliveira y Oliveira, 2008) con el objetivo de aumentar la productividad y rentabilidad del rebaño.Dentro de las biotecnologías se debe resalta...

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“…The reactivation could possibly have been due to the dilution effect, the osmolarity of the A CL being only199 mOsm/kg, or to the addition of more glucose. Verstegen et al (2005) [14] report how cold-stored spermatozoa that had become non-motile after a certain period of preservation were reactivated and motility parameters restimulated by centrifuging the sample and adding more of the extender.…”

Section: Discussionmentioning

confidence: 99%

“…Their conception rates obtained with semen stored for 5, 6, 7, and 9 days were 50.0% (1/2), 0% (0/5), 33.3% (1/3), and 0% (0/1), respectively, while the numbers of pups obtained with semen stored for 5 and 7 days were two and one. Verstegen et al (2005) [14] report a pregnancy rate of 60% with semen that had been preserved chilled for 7-11 days. The average litter size was 2.3±1.4 by single AI (five out of ten bitches pregnant) and 4.6±2.8 by two AIs at an interval of 48 hours (seven out of ten bitches pregnant).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Induced immotility during long-term storage at +5°C does not prolong survival of dog spermatozoa

Shahiduzzaman

Linde‐Forsberg

2007

Theriogenology

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The days are not so far away when sex sorted preserved liquid semen will be available in drug stores saying "Let me stay cool, I will give you beautiful puppies".To my parents (Abdus Satter and Jamela Khatun ) Preservation of canine semen by extension and chilling has provided the AI veterinarians with an opportunity to overcome limitations of both time and space. Several parameters are assessed to predict the fertility of canine spermatozoa for a given sample. Motility is one of them. Spermatozoa stay immotile while preserved in CLONE (Cryogenetic Laboratories of New England, Inc, USA) Chilled Semen Extender whereas they maintain motility over the whole 23 days period of study while preserved in a Tris egg yolk glucose extender at 5°C.The aim of this study was to investigate whether the immotility induced by the CLONE chilled semen extender prolongs the lifespan of dog spermatozoa stored at 5°C, compared with a Tris-egg yolk-glucose (TG) extender, which maintains motility. Semen from eleven dogs was pooled, split in four aliquots, centrifuged, and the supernatants removed. The four sperm pellets were mixed with a TG extender; with the CLONE chilled semen (CL) extender; with TG extender mixed with TG but without egg yolk and with less glucose (TG+A TG ); or with the CLONE extender mixed with the CLONE activator (CL+A CL ) to a final sperm concentration of 200 x 10 6 spermatozoa / mL. The samples were stored at 5ºC for 23 days and examined twelve times for sperm motility by computer-assisted sperm analysis (CASA), as well as for plasma membrane and acrosome integrity, glucose consumption, and DNA fragmentation index (DFI). The experiment was performed in triplicate. The third replicate was examined for presence of bacteria. For the statistical calculations, the 23 experimental days were divided into three periods, the first period comprising days 1-8, the second period days 9-14, and the third period days 15-23. Total motility (TM%) did not differ between extenders in the first period, but was higher in TG and TG+A TG compared with CL+A CL in the second and third periods, and with CL in the third period (P<0.05). Progressive motility (PM%) was higher in TG (P<0.0001), TG+A TG (P=0.0007) and CL+A CL (P=0.0176) than in CL in the first period. Both TG and TG+A TG maintained higher PM% than did CL and CL+A CL in the second and third period. Tris-egg yolkglucose and TG+A TG maintained higher straight line velocity (VSL) and average path velocity (VAP) compared with CL and CL+A CL during the whole experimental period. Curvilinear velocity (VCL) was higher in TG, CL, and TG+A TG than in CL+A CL samples (P<0.05) in the first and third periods. No differences in lateral head displacement (LHD) were observed between extenders or over time. Acrosome and plasma membrane integrity decreased over time, but there were no differences among the extenders in the first and second periods (P>0.05). In the third period, however, TG, TG+A TG , and CL maintained acrosome and plasma membrane integrity better than CL+A CL . Glucose consu...

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Long-term motility and fertility conservation of chilled canine semen using egg yolk added Tris–glucose extender: In vitro and in vivo studies

Cited by 63 publications

References 15 publications

The Efficacy of In Vitro Sperm Tests in Predicting Pregnancy Success after Artificial Insemination in the Bitch

The Efficacy of In Vitro Sperm Tests in Predicting Pregnancy Success after Artificial Insemination in the Bitch

Viabilidad de espermatozoides ovinos mantenidos a 5º y 15ºC en diferentes sistemas de refrigeración

Induced immotility during long-term storage at +5°C does not prolong survival of dog spermatozoa

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