Long‐term muscle‐derived cell culture: multipotency and susceptibility to cell death stimuli

Abstract

Improvement in the yield of adult organism stem cells, and the ability to manage their differentiation and survival potential are the major goals in their application in regenerative medicine and in the adult stem cell research. We have demonstrated that adult rabbit muscle-derived cell lines with an unlimited proliferative potential in vitro can differentiate into myogenic, osteogenic, adipogenic and neurogenic lineages. Studies of cell survival in vitro showed that differentiated cells, except neurogenic ones, are more resistant to apoptosis inducers compared to proliferating cells. Resistance to death signals correlated with the level of protein kinase AKT phosphorylation. Skeletal muscle-derived cell lines can be multipurpose tools in therapy. Enhanced resistance of differentiated cells to certain types of damage shows their potential for long-term survival and maintenance in an organism. This article was published online on 29 January 2013. An error was subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected 6 March 2013.

JNK implication in adipocyte-like cell death induced by chemotherapeutic drug cisplatin

“…It is known that muscle-derived stem cells can be cultured in vitro as permanent lines and under certain conditions they give rise to different kinds of progeny cells, making them a suitable model for pharmacological and toxicological studies. We have shown that adult rabbit muscle-derived Myo cell lines with an unlimited proliferative potential in vitro can differentiate into myogenic, osteogenic, adipogenic and neurogenic lineages (Kalvelyte et al, 2013). Our data prove that proliferating and differentiated cells have different susceptibility to cytotoxic agents, e.g.…”

“…Our data prove that proliferating and differentiated cells have different susceptibility to cytotoxic agents, e.g. Myo cells differentiated into myogenic, osteogenic and adipogenic lineages appeared to be more resistant to apoptosis inducers compared to proliferating cells (Kalvelyte et al, 2013).…”

“…0121, 2004-07-09 and0171, 2007-10-31) was issued by Lithuanian Food and Veterinary Office. After cell isolation from the muscle, slowly adhering cell population was selected by the replating procedure of non-adherent cells (Kalvelyte et al, 2013). IMDM supplemented with 10% FBS and antibiotics (penicillin 100 U/mL, streptomycin 100 μg/mL) was used as growth medium.…”

Stem cell death and survival in heart regeneration and repair

“…Thus, it is also worth investigating mechanisms by which normal cells acquire resistance to death induction and stress conditions during differentiation. For example, mouse, rabbit and human differentiating muscle cells display differentiation-induced resistance to genotoxic agents, as well as diminished susceptibility to apoptosis [100–104]. Conversely, some investigators suggested that undifferentiated cells are more resistant to oxidative stress, probably because of a higher level of intracellular redox system, which is characteristic of stem cells [105, 106].…”

“…The major mediator of this pathway is the AKT kinase which is implicated in stem cell and myocyte survival, myogenesis, in part by promoting the expression of various molecules including the myogenin gene [109–112]. Moreover, both AKT1 and AKT2 proteins and kinase activities are upregulated during myogenic differentiation and are tightly associated with apoptosis-resistance [104, 110, 112, 113]. In particular, AKT signaling in the infarcted heart can block apoptosis, decrease infarct size, and increase left ventricular wall thickness compared to pre-infarction condition [114, 115].…”