Experiments were carried out to test the capacity for a laboratory-scale biofilter operated at an elevated temperature level (∼50°C) to remove an air stream containing β-caryophyllene, a naturally occurring sesquiterpene of environmental concern emitted from wood-related industrial facilities. A water jacket was used to maintain high temperatures in a laboratory-scale biofilter. Inocula, pollutant loading and nutrient supply rate effects were evaluated over 84 days of biofilter operation. The start-up process took over two months when citrus peels were used as inocula while a relatively short start-up period was achieved after introducing forest compost products. While using a sparged-gas bioreactor to cultivate an enrichment culture for 97 days, removal efficiencies in excess of 80% were observed after 18 days. At empty bed contact times of 50 s and at a pollutant loading rate of 3.05 mg C/L/hr, removal efficiency levels reached 90% and the elimination capacity level reached 2.29 mg C/L/hr, corresponding to an elimination capacity of 2.60 mg β-caryophyllene/L/hr. Collectively, these results demonstrate that β-caryophyllene can be successfully removed from contaminated air using a biofilter operated at a high temperature (∼50°C), expanding the temperature range within which biofilters are known to biodegrade sesquiterpenes.