Long‐term oral administration of glucosamine or chondroitin sulfate reduces destruction of cartilage and up‐regulation of MMP‐3 mRNA in a model of spontaneous osteoarthritis in Hartley guinea pigs

Taniguchi, Shin; Ryu, Junnosuke; Seki, Masaaki; Sumino, Takanobu; Tokuhashi, Yasuaki; Esumi, Mariko

doi:10.1002/jor.22003

Cited by 47 publications

(36 citation statements)

References 23 publications

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“…Our observation that chondrocyte apoptosis is higher in the medial side and largely localised in the deep zone (DZ) of AC supports other studies of spontaneous OA, both in guinea pigs [23] and some human studies [19], but differs considerably from results of studies where OA was induced. Thus, animal models of surgical/injury-induced OA ( i.e.…”

Section: Discussionsupporting

confidence: 88%

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Increased Chondrocyte Apoptosis Is Associated with Progression of Osteoarthritis in Spontaneous Guinea Pig Models of the Disease

Zamli

Adams

Tarlton

et al. 2013

IJMS

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Osteoarthritis (OA) is the most common joint disease characterised by degradation of articular cartilage and bone remodelling. For almost a decade chondrocyte apoptosis has been investigated as a possible mechanism of cartilage damage in OA, but its precise role in initiation and/or progression of OA remains to the determined. The aim of this study is to determine the role of chondrocyte apoptosis in spontaneous animal models of OA. Right tibias from six male Dunkin Hartley (DH) and Bristol Strain 2 (BS2) guinea pigs were collected at 10, 16, 24 and 30 weeks of age. Fresh-frozen sections of tibial epiphysis were microscopically scored for OA, and immunostained with caspase-3 and TUNEL for apoptotic chondrocytes. The DH strain had more pronounced cartilage damage than BS2, especially at 30 weeks. At this time point, the apoptotic chondrocytes were largely confined to the deep zone of articular cartilage (AC) with a greater percentage in the medial side of DH than BS2 (DH: 5.7%, 95% CI: 4.2–7.2), BS2: 4.8%, 95% CI: 3.8–5.8), p > 0.05). DH had a significant progression of chondrocyte death between 24 to 30 weeks during which time significant changes were observed in AC fibrillation, proteoglycan depletion and overall microscopic OA score. A strong correlation (p ≤ 0.01) was found between chondrocyte apoptosis and AC fibrillation (r = 0.3), cellularity (r = 0.4) and overall microscopic OA scores (r = 0.4). Overall, the rate of progression in OA and apoptosis over the study period was greater in the DH (versus BS2) and the medial AC (versus lateral). Chondrocyte apoptosis was higher at the later stage of OA development when the cartilage matrix was hypocellular and highly fibrillated, suggesting that chondrocyte apoptosis is a late event in OA.

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Section: Discussionsupporting

confidence: 88%

“…Chondrocyte death by apoptosis has been reported both in human [13,17–20] and in animal models of OA [3,9,21–23]. In these studies, chondrocyte apoptosis was detected by caspase-3 expression and TUNEL, and confirmed by electron microscopic examination.…”

Section: Introductionmentioning

confidence: 97%

Increased Chondrocyte Apoptosis Is Associated with Progression of Osteoarthritis in Spontaneous Guinea Pig Models of the Disease

Zamli

Adams

Tarlton

et al. 2013

IJMS

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“…Этот важ-ный механизм действия ХС подтвержден в эксперимен-тальных исследованиях: на модели адъювантного артрита у крыс, ХС достоверно уменьшал тяжесть артрита, пода-вляя продукцию провоспалительных цитокинов, СРБ и фагоцитарную активность [35]. В другой эксперименталь-ной модели остеоартроза назначение глюкозамина и ХС снижало экспрессию мРНК матричной металопероксида-зы (ММП), в частности ММП-3, и уменьшало дегенерацию суставного хряща [36].…”

Section: ключевые слова: дорсалгия спондилоартроз структурно-модифициunclassified

The place of chondroprotectors in the treatment of osteoarthritis of the spine

Пизова¹

2016

Med. sov.

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“…The applicant also provided studies in animals on the effect of glucosamine on cartilage formation/degradation (Chen et al, 2010;Meulyzer et al, 2009;Naito et al, 2010;Oegema et al, 2002;Taniguchi et al, 2012). Four studies were provided in animal models of OA, i.e.…”

Section: Mechanistic Studiesmentioning

confidence: 99%

Scientific Opinion on the substantiation of a health claim related to glucosamine and maintenance of normal joint cartilage pursuant to Article 13(5) of Regulation (EC) No 1924/2006

Publication¹

2012

EFSA Journal

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Following an application from Merck Consumer Healthcare, submitted pursuant to Article 13(5) of Regulation (EC) via the Competent Authority of Belgium, the Panel on Dietetic Products, Nutrition and Allergies was asked to deliver an opinion on the scientific substantiation of a health claim related to glucosamine, formulated as glucosamine sulphate or hydrochloride, and maintenance of normal joint cartilage. Glucosamine is sufficiently characterised. The claimed effect is "contributes to the maintenance of normal joint cartilage". The target population as proposed by the applicant is the general population, and in particular people exposing their joints to high mechanical load and people with joint cartilage deterioration due to normal ageing. The Panel considers that the maintenance of normal joint cartilage is a beneficial physiological effect. The applicant provided references to studies in patients with osteoarthritis, in healthy subjects, in animals and in vitro as being pertinent to the health claim. In weighing the evidence, the Panel took into account that no human studies were provided from which conclusions could be drawn on the effect of dietary glucosamine on the maintenance of cartilage in individuals without osteoarthritis, and that the evidence provided in the in vitro and animal studies in support of the biological plausibility for a possible contribution of dietary glucosamine to the maintenance of joint cartilage in humans is weak. The Panel concludes that a cause and effect relationship has not been established between the consumption of glucosamine and maintenance of normal joint cartilage in individuals without osteoarthritis. © European Food Safety Authority, 2012 The scope of the application was proposed to fall under a health claim based on newly developed scientific evidence. KEY WORDSThe food constituent that is the subject of the health claim is glucosamine, formulated as glucosamine sulphate or hydrochloride. The Panel considers that glucosamine is sufficiently characterised.The claimed effect is "contributes to the maintenance of normal joint cartilage". The target population as proposed by the applicant is the general population, and in particular people exposing their joints to high mechanical load, for example through extensive sports activities or obesity, and people with joint cartilage deterioration due to normal ageing. From the information provided, the Panel notes that the claimed effect relates to the maintenance of joint cartilage. The Panel considers that the maintenance of normal joint cartilage is a beneficial physiological effect.In the original application, the applicant identified a total of 61 references as being pertinent to the health claim. These references comprised 20 references describing 12 human intervention studies, 13 reviews on the effects of glucosamine supplementation on maintenance of joints, four animal studies on the effects of glucosamine on joints, 14 in vitro studies on the effects of glucosamine on cartilage cells/tissues, nine studies i...

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Long‐term oral administration of glucosamine or chondroitin sulfate reduces destruction of cartilage and up‐regulation of MMP‐3 mRNA in a model of spontaneous osteoarthritis in Hartley guinea pigs

Cited by 47 publications

References 23 publications

Increased Chondrocyte Apoptosis Is Associated with Progression of Osteoarthritis in Spontaneous Guinea Pig Models of the Disease

Increased Chondrocyte Apoptosis Is Associated with Progression of Osteoarthritis in Spontaneous Guinea Pig Models of the Disease

The place of chondroprotectors in the treatment of osteoarthritis of the spine

Scientific Opinion on the substantiation of a health claim related to glucosamine and maintenance of normal joint cartilage pursuant to Article 13(5) of Regulation (EC) No 1924/2006

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