Long-Term Organic Nutrient Management Fosters the Eubacterial Community Diversity in the Indian Semi-arid Alfisol as Revealed by Length Heterogeneity–PCR

Balachandar, D.; Doud, Melissa; Schneper, Lisa; Mills, DeEtta K.; Mathee, Kalai

doi:10.1080/00103624.2013.841919

Cited by 11 publications

(4 citation statements)

References 43 publications

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“…The influence of the molecular ecology and culturing method on the diversity, composition and dynamics of the microbial community have been investigated using cell cultures, colony counting, denaturing gradient gel electrophoresis [9,10], temperature gradient gel electrophoresis [11–13], microarray [14] and length heterogeneity polymerase chain reaction [15,16]. However, a complete understanding of the microbial community remains elusive [17].…”

Section: Introductionmentioning

confidence: 99%

High-Throughput Sequencing of Microbial Community Diversity and Dynamics during Douchi Fermentation

Yang

2016

PLoS ONE

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Douchi is a type of Chinese traditional fermented food that is an important source of protein and is used in flavouring ingredients. The end product is affected by the microbial community present during fermentation, but exactly how microbes influence the fermentation process remains poorly understood. We used an Illumina MiSeq approach to investigate bacterial and fungal community diversity during both douchi-koji making and fermentation. A total of 181,443 high quality bacterial 16S rRNA sequences and 221,059 high quality fungal internal transcribed spacer reads were used for taxonomic classification, revealing eight bacterial and three fungal phyla. Firmicutes, Actinobacteria and Proteobacteria were the dominant bacterial phyla, while Ascomycota and Zygomycota were the dominant fungal phyla. At the genus level, Staphylococcus and Weissella were the dominant bacteria, while Aspergillus and Lichtheimia were the dominant fungi. Principal coordinate analysis showed structural separation between the composition of bacteria in koji making and fermentation. However, multivariate analysis of variance based on unweighted UniFrac distances did identify distinct differences (p <0.05), and redundancy analysis identified two key genera that are largely responsible for the differences in bacterial composition between the two steps. Staphylococcus was enriched in koji making, while Corynebacterium was enriched in fermentation. This is the first investigation to integrate douchi fermentation and koji making and fermentation processes through this technological approach. The results provide insight into the microbiome of the douchi fermentation process, and reveal a structural separation that may be stratified by the environment during the production of this traditional fermented food.

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Section: Introductionmentioning

confidence: 99%

High-Throughput Sequencing of Microbial Community Diversity and Dynamics during Douchi Fermentation

Yang

2016

PLoS ONE

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“…We measured these six variables from four long-term nutrient management adopted soils (control, inorganic fertilizer-applied, organic manure amended, and integrated nutrient management adopted). Based on the literature and our previous works, the continuous use of organic and inorganic nutrient amendments could significantly alter the above mentioned biological attributes and largely SOC (Preethi et al, 2012;Chinnadurai et al, 2013;Balachandar et al, 2014;Chinnadurai et al, 2014;Tamilselvi et al, 2015;Balachandar et al, 2016). All these biological attributes were reported highest in INM and OM, whereas the IC and control recorded on par values or sometimes IC was higher than control.…”

Section: Soil Biological Attributes and Soil Biological Quality Indexmentioning

confidence: 90%

Correlation between Carbon dioxide Evolution and Biological Quality Index of Long-term Nutrient Management Adopted Soils

Priya¹,

Malarkodi²,

Karthikeyan³

et al. 2020

Madras Agricultural Journal

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Soil respiration in terms of carbon dioxide (CO 2) evolution is a potential biological indicator to assess the soil quality as influenced by different agricultural practices. However, the relation between the CO 2 evolution and the biological attributes of soil is a multitude and hence less resolved. The purpose of the study is to relate the measured CO 2 values with the biological quality indicators of the soil. Four long-term nutrient management treatments (organic manure amended, OM, integrated nutrient management enforced, INM, synthetic fertilizer applied, IC, and unfertilized control, Control) from 100-years old permanent manurial experiment was used for this study. The soils were analyzed for soil organic carbon, microbial biomass carbon, labile carbon, protein index, dehydrogenase activity, and substrate-induced respiration and calculated the soil biological quality index (SBQI) by quadrantplot method. All the soils were measured for the flush of CO 2 immediately after wetting the dry soil by three methods viz., alkali trap method, infra-red probe-based flux apparatus, and multi-gas analyzer. The results revealed that INM had a positive influence to improve all the six biological attributes followed by OM, while the IC and Control had at par levels of all the assessed variables. The SBQI also has high resolution to discriminate against the soils and INM > OM > IC ≈ Control is the order being observed. The CO 2 evolved from these four soils had a strong positive correlation with all the assessed biological health indicators. The regression analysis revealed that SBQI had a significant positive relation with alkali trap CO 2 (R 2 0.82), flux CO 2 (R 2 0.75), and multi-gas analyzer CO 2 (R 2 0.79). The present results suggest that analyzing CO 2 evolution could be a simple method to relate the overall biological quality of the soil. Hence, developing a simple tool to measure the soil CO 2 evolution could help the farmers to know the biological quality of the soil .

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“…Our ndings also showed that resource conservation practices have higher microbial biomass carbon (MBC) content compared to CC (Table 2). Balanced nutrient inputs and supplemented by resource conservation practices play a crucial role for enhancement of soil organic carbon (SOC) and MBC (Cookson et al, 2007;Balachandar et al, 2014). The higher content of MBC in green manuring treatment might be due to presence of higher shoot and root biomass turn- over (Mandal et al, 2007;Liu et al, 2013;Shahid et al, 2017), which signi cantly increases carbon input (Manna et al, 2005;Lal, 2015).…”

Section: Impact Of Resource Conservation On Soil Carbon and Nitrogen Fractionsmentioning

confidence: 99%

Long Term Resource Conservation Technologies Sustained Yield, Microbial Activities and Soil Physico-chemical Properties in Rice-green Gram Cropping System

Dash

Bhattacharyya

Shahid

et al. 2021

Preprint

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PurposeMicrobial communities in rhizospheric soil play a significant role in sustaining the soil quality and also recognized as key ecological indicators to assess the soil health. MethodsWe studied the long-term effects of resource conservation technologies on functional microbial diversity and their interactions with soil biochemical properties and enzymatic activities in tropical rice-green gram cropping system. The experiment included conventional practice (CC), brown manuring (BM), green manuring (GM), wet direct drum sowing (WDS), zero tillage (ZT), green manuring-customized leaf colour chart based-N application (GM-CLCC N) and biochar (BC) treatments. ResultsThe result revealed that microbial biomass nitrogen (N), carbon (C) and phosphorus (P) in GM practice increased by 23.3, 37.7 and 35.1%, respectively over CC. The Shannon index and McIntosh index were higher by 86.9% and 29.2% in GM as compared to conventional practice and significantly correlated with microbial biomass (C & P) and soil microbial populations whereas, Shannon index was positively correlated with the microbial biomass (C, N & P) and soil enzyme activities. Principal component analysis showed a significant separate cluster among the treatments amended with and without biomass addition. ConclusionsMoreover, dominance of carbon utilizing microbes in biomass amended treatments indicated that these could supply good amount of labile carbon sources on real time basis for microbial activity. Which may protect the stable carbon fraction in soil, hence could support higher build-up of carbon in long run and could offer sustainable yield under rice-green gram soil.

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Long-Term Organic Nutrient Management Fosters the Eubacterial Community Diversity in the Indian Semi-arid Alfisol as Revealed by Length Heterogeneity–PCR

Cited by 11 publications

References 43 publications

High-Throughput Sequencing of Microbial Community Diversity and Dynamics during Douchi Fermentation

High-Throughput Sequencing of Microbial Community Diversity and Dynamics during Douchi Fermentation

Correlation between Carbon dioxide Evolution and Biological Quality Index of Long-term Nutrient Management Adopted Soils

Long Term Resource Conservation Technologies Sustained Yield, Microbial Activities and Soil Physico-chemical Properties in Rice-green Gram Cropping System

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