Long-Term Persistence of Immunoglobulin A (IgA) and IgM Antibodies against Human Cytomegalovirus in Solid-Organ Transplant Recipients

Eing, Bodo R.; Baumeister, H.; Kuehn, Joachim E.; May, Guenter

doi:10.1128/cdli.6.4.621-623.1999

Cited by 5 publications

(1 citation statement)

References 17 publications

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“…This could be due to a persistence of IgM longer than the persistence of the virus in the blood after active CMV infection. In fact, viral DNA can persist in peripheral blood leukocytes for as long as 6 months after primary CMV infection in immunocompetent subjects [Revello et al, 1998;Zanghellini et al, 1999], whereas IgM are detectable for 30 weeks in immunocompetent subjects [Stagno et al, 1985] and even longer in immunocompromised patients [Allen et al, 1991;Eing et al, 1999]. On the other hand, in 19 IgM-negative patients with cirrhosis qPCR gave a positive result.…”

Section: Resultsmentioning

confidence: 99%

Laboratory signs of acute or recent cytomegalovirus infection are common in cirrhosis of the liver

et al. 2000

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Human cytomegalovirus (CMV) is an ubiquitous pathogen that can cause severe and often fatal infections in immunocompromised patients. Patients with cirrhosis often show various degrees of impaired cellular immunity that could lead to acute CMV reactivation. The aim of the present study was to determine whether laboratory findings of active CMV infections are common in patients with cirrhosis. Fifty-five patients with cirrhosis were studied for acute CMV infection by virological (antigenemia and quantitative polymerase chain reaction in polymorphonuclear leukocytes) and serological (detection of anti-CMV IgM by immunoblot) methods. The same tests were carried out on 50 blood donors and on 20 chronic hepatitis patients, considered as control populations. Acute or recent CMV infection had occurred in 31 (56%) of 55 patients with cirrhosis, whereas only 1 out of 20 (5%) patients with chronic non-cirrhotic liver disease and none of the 50 blood donors had laboratory signs of active CMV infection. The difference between patients with cirrhosis and the control groups was significant (P < 0.001, chi(2) test). CMV in patients with cirrhosis was not related to age, gender, hepatitis C virus infection or hepatocellular carcinoma. There was no significant correlation between impairment of liver function and the presence of active CMV infection. Patients with cirrhosis should be considered at risk for CMV infection, that seems to be mild and asymptomatic.

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Section: Resultsmentioning

confidence: 99%

Laboratory signs of acute or recent cytomegalovirus infection are common in cirrhosis of the liver

et al. 2000

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Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein‐Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens

Engelmann

Petzold

Kosinska

et al. 2008

Journal of Medical Virology

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Rapid diagnosis of human herpesvirus primary infections or reactivations is facilitated by quantitative PCRs. Quantitative PCR assays with a standard thermal cycling profile permitting simultaneous detection of herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and human herpesvirus 6 (HHV6) DNA were developed and validated for diagnostic use. High specificity and sensitivity were achieved and the new PCR assays correlated well with commercial PCR assays. Twenty two thousand eight hundred sixty eight PCR tests were undertaken on specimens obtained from immunosuppressed patients. DNAemia was frequent with EBV (43.5%), HHV6 (32.4%), CMV (12.8%), and VZV (12.9%). As already described for EBV and CMV, high virus loads of HHV6 and VZV were associated with clinical symptoms and poor clinical outcome, for example, three of four patients with VZV virus loads >10(5) copies/ml died. A high proportion of lower respiratory specimens was positive for EBV- (38.8%), HHV6- (29.4%), and CMV-DNA (18.2%). For CMV, infection was confirmed in 66.7% of patients by virus isolation or positive pp65 antigenaemia. Differentiation of HHV6A, -B and HSV-1, -2 by melting curve analysis revealed that HHV6A and HSV-2 represented only 1.8% and 3.3% of all positive specimens, respectively. In conclusion, these results indicate significant improvements for the early diagnosis of primary infections or reactivations of five human herpesviruses especially in immunosuppressed patients. Detection of coinfections with multiple herpesviruses is facilitated. Quantitative results enable monitoring of virus load during antiviral therapy. A standard thermal cycling profile permits time and cost effective use in a routine diagnostic setting.

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High intrahepatic HHV-6 virus loads but neither CMV nor EBV are associated with decreased graft survival after diagnosis of graft hepatitis

Pischke

Gösling

Schlué

et al. 2012

Journal of Hepatology

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Long-Term Persistence of Immunoglobulin A (IgA) and IgM Antibodies against Human Cytomegalovirus in Solid-Organ Transplant Recipients

Cited by 5 publications

References 17 publications

Laboratory signs of acute or recent cytomegalovirus infection are common in cirrhosis of the liver

Laboratory signs of acute or recent cytomegalovirus infection are common in cirrhosis of the liver

Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein‐Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens

High intrahepatic HHV-6 virus loads but neither CMV nor EBV are associated with decreased graft survival after diagnosis of graft hepatitis

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