Long-Term Persistence of the Nuclear Polyhedrosis Virus of the Douglas-Fir Tussock Moth,
Orgyia pseudotsugata
(Lepidoptera: Lymantriidae), in Forest Soil

Thompson, Clarence G.; Scott, Donald W.; Wickman, Boyd E.

doi:10.1093/ee/10.2.254

Cited by 69 publications

(30 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Clearly, there was no significant reduction in the number of biologically active polyhedra between years 2 and 3. Persistence of baculovirus polyhedra in soil is a well-documented phenomenon (Thomas et al 1972, Jaques 1975, Thompson et al 1981.…”

Section: Discussionmentioning

confidence: 99%

Field Studies of the Co-Occlusion Strategy with a Genetically Altered Isolate of the Autographa californica Nuclear Polyhedrosis Virus

Wood

Hughes

Shelton

1994

Environmental Entomology

View full text Add to dashboard Cite

The first field study of a genetically altered virus in the United States was performed with an isolate of the Autographa californica nuclear polyhedrosis virus (AcMNPV), which lacks a polyhedrin gene. In the first year of the study, three applications of 7.4 x 1011 AcMNPV polyhedra containing 48% genetically altered and 52% wild-type virus particles (co-occluded) were made on a O.I-ha circular plot of cabbage plants. The application area was surrounded by a 0.7-ha circular buffer zone. Before each application, the plants in the application area were infested with 4,500 third-ins tar Trichoplusia ni (Hubner) larvae. After each application, 100% of the T. ni test larvae sampled 5 dafter infection were infected with AcMNPV and produced progeny polyhedra containing an average of 42 :t 17.6% genetically altered virus particles. At the end of the 1st yr, the progeny polyhedra population in the application area was estimated at 1.6 x 10 13 polyhedra. In the 2nd yr, the application and buffer sites were replanted with cabbage plants. At four times during the growing season, the plants were seeded with T. ni larvae or eggs. Less than 2% of the test larvae became infected with AcMNPV. Polyhedra were extracted from soil samples collected in the application and buffer areas. Using neonate larval bioassays with the soil extracts, it was estimated that the soil in the application and buffer areas contained an average of 1,652 ± 3,370 and 832 ± 2,539 biologically active polyhedra per gram dry weight, respectively. Seventy-five larvae infected with polyhedra extracted from application area soil samples produced progeny polyhedra containing a mean of9 :t 19% genetically altered virus particles. In the 3rd yr, the application area soil samples contained an average of 1,671 :t 3,274 biologically active polyhedra per gram dry weight. Eighty-four progeny polyhedral samples contained a mean of 6 ± 14% genetically altered virus particles. The co-occlusion strategy did not alter the environmental persistence of the polyhedra containing both wild-type and polyhedrin-minus virus particles. However, the data show a decline in the percent of polyhedrin-minus particles in the polyhedra and demonstrate that the persistence of a polyhedrin-minus virus in a cycling virus population is limited by the co-occlusion process. The environmentally desirable attributes of using the co-occlusion process for genetically enhanced baculovirus pesticides and possible problems are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Field Studies of the Co-Occlusion Strategy with a Genetically Altered Isolate of the Autographa californica Nuclear Polyhedrosis Virus

Wood

Hughes

Shelton

1994

Environmental Entomology

View full text Add to dashboard Cite

show abstract

“…The rodshaped virions, occluded in a proteinaceous occlusion body (OB) (Fig. 1) that can persist outside the host for a considerable period of time (Clark 1956, Thompson et al 1981) must be ingested or vertically transmitted to cause infection (Cory and Myers 2003). Baculoviruses have been reported exclusively from arthropods, and have been recovered and genome sequenced so far only from Diptera, Hymenoptera, and Lepidoptera (Herniou et al 2004).…”

Section: Baculoviruses and Eruptive Forest Insectsmentioning

confidence: 99%

A brief review of the past use of baculoviruses for the management of eruptive forest defoliators and recent developments on a sawfly virus in Canada

Moreau¹,

Lucarotti²

2007

The Forestry Chronicle

View full text Add to dashboard Cite

show abstract

“…While NPV occlusion bodies (OBs) have been found to persist in soil for years (Thompson et al, 1981), the fate of OBs in aquatic environments is not known. If the OBs are susceptible to breakdown from enzymatic activities or biochemical processes in aquatic bottom substrates, indigenous competent bacteria could take up viral DNA containing the genetic inserts via natural transformation.…”

Section: Introductionmentioning

confidence: 99%

Comparative Effects of a Genetically Engineered Insect Virus and a Growth-Regulating Insecticide on Microbial Communities in Aquatic Microcosms

Kreutzweiser

England

Shepherd

et al. 2001

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

Long-Term Persistence of the Nuclear Polyhedrosis Virus of the Douglas-Fir Tussock Moth, Orgyia pseudotsugata (Lepidoptera: Lymantriidae), in Forest Soil

Cited by 69 publications

References 0 publications

Field Studies of the Co-Occlusion Strategy with a Genetically Altered Isolate of the Autographa californica Nuclear Polyhedrosis Virus

Field Studies of the Co-Occlusion Strategy with a Genetically Altered Isolate of the Autographa californica Nuclear Polyhedrosis Virus

A brief review of the past use of baculoviruses for the management of eruptive forest defoliators and recent developments on a sawfly virus in Canada

Comparative Effects of a Genetically Engineered Insect Virus and a Growth-Regulating Insecticide on Microbial Communities in Aquatic Microcosms

Contact Info

Product

Resources

About