Long-term predictive value of salivary microbial diagnostic tests in children

Ollila, Päivi; Larmas, Markku

doi:10.1007/bf03321592

Cited by 12 publications

(8 citation statements)

References 19 publications

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“…Candida albicans is the most commonly isolated candida species in the oral cavity and constitute up to 80% of the clinical isolates [20]. Furthermore, candida has been ascribed a significant predictive value for dental caries in children [21]. The majority of the lactobacilli strains were fully identified with biochemical analysis using the API 50 CH system while a satisfactory identification of the L .…”

Section: Discussionmentioning

confidence: 99%

Growth inhibition of oral mutans streptococci and candida by commercial probiotic lactobacilli - an in vitro study

et al. 2010

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BackgroundProbiotic bacteria are suggested to play a role in the maintenance of oral health. Such health promoting bacteria are added to different commercial probiotic products. The aim of the study was to investigate the ability of a selection of lactobacilli strains, used in commercially available probiotic products, to inhibit growth of oral mutans streptococci and C. albicans in vitro.MethodsEight probiotic lactobacilli strains were tested for growth inhibition on three reference strains and two clinical isolates of mutans streptococci as well as two reference strains and three clinical isolates of Candida albicans with an agar overlay method.ResultsAt concentrations ranging from 109 to 105 CFU/ml, all lactobacilli strains inhibited the growth of the mutans streptococci completely with the exception of L. acidophilus La5 that executed only a slight inhibition of some strains at concentrations corresponding to 107 and 105 CFU/ml. At the lowest cell concentration (103 CFU/ml), only L. plantarum 299v and L. plantarum 931 displayed a total growth inhibition while a slight inhibition was seen for all five mutans streptococci strains by L. rhamnosus LB21, L. paracasei F19, L. reuteri PTA 5289 and L. reuteri ATCC 55730. All the tested lactobacilli strains reduced candida growth but the effect was generally weaker than for mutans streptococci. The two L. plantarum strains and L. reuteri ATCC 55730 displayed the strongest inhibition on Candida albicans. No significant differences were observed between the reference strains and the clinical isolates.ConclusionThe selected probiotic strains showed a significant but somewhat varying ability to inhibit growth of oral mutans streptococci and Candida albicans in vitro.

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Section: Discussionmentioning

confidence: 99%

Growth inhibition of oral mutans streptococci and candida by commercial probiotic lactobacilli - an in vitro study

et al. 2010

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“…This was attributed to its acidogenicity, capabilities of forming hyphae and the secretion of dentine‐degrading enzymes . Some studies support a significant association between dental caries and the presence and levels of C. albicans in saliva . A study following 183 children over 7 years showed that early colonization with Candida was associated with caries onset in primary molars, but not in permanent first molars .…”

Section: Microorganisms In Saliva As Biomarkers Of Cariesmentioning

confidence: 99%

“…Some studies support a significant association between dental caries and the presence and levels of C. albicans in saliva . A study following 183 children over 7 years showed that early colonization with Candida was associated with caries onset in primary molars, but not in permanent first molars . In some other studies, however, association between caries and Candida was not supported .…”

Section: Microorganisms In Saliva As Biomarkers Of Cariesmentioning

confidence: 99%

Salivary biomarkers for dental caries

Gao¹,

Jiang²,

Koh³

et al. 2015

Periodontology 2000

176

123

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As a highly prevalent multifactorial disease, dental caries afflicts a large proportion of the world's population. As teeth are constantly bathed in saliva, the constituents and properties of this oral fluid play an essential role in the occurrence and progression of dental caries. Various inorganic (water and electrolytes) and organic (proteins and peptides) components may protect teeth from dental caries. This occurs via several functions, such as clearance of food debris and sugar, aggregation and elimination of microorganisms, buffering actions to neutralize acid, maintaining supersaturation with respect to tooth mineral, participation in formation of the acquired pellicle and antimicrobial defense. Modest evidence is available on the associations between dental caries and several salivary parameters, including flow rate, buffering capacity and abundance of mutans streptococci. Despite some controversial findings, the main body of the literature supports an elevated caries prevalence and/or incidence among people with a pathologically low saliva flow rate, compromised buffering capacity and early colonization or high titer of mutans streptococci in saliva. The evidence remains weak and/or inconsistent on the association between dental caries and other saliva parameters, such as other possible cariogenic species (Lactobacillus spp., Streptococcus sanguis group, Streptococcus salivarius, Actinomyces spp. and Candida albicans), diversity of saliva microbiomes, inorganic and organic constituents (electrolytes, immunoglobulins, other proteins and peptides) and some functional properties (sugar clearance rate, etc.). The complex interactions between salivary components and functions suggest that saliva has to be considered in its entirety to account for its total effects on teeth.

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“…Recently, C. albicans has been shown to cause caries (Nikawa et al, 2003). Streptococcus mutans, lactic acid bacteria, and actinomycetes have traditionally been recognized as the main factors causing caries bacteria in the oral cavity; however, C. albicans is frequently present in caries-active groups, and the rate of detection of C. albicans in the oral cavity is much higher than that in those who are caries-free (Ollila and Larmas, 2008;Raja et al, 2010). C. albicans has been detected in children's dentine caries at a rate of 71-97% (de Carvalho et al, 2006), indicating the importance of this pathogen in causing caries.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Candida albicans in the oral cavity of children aged 3-5 years of Uygur and Han nationality and their genotype in caries-active groups

Lin

et al. 2015

Genet. Mol. Res.

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ABSTRACT. We analyzed the distribution of Candida albicans in the oral cavity of 3-5-year-old children of Uygur and Han nationalities as well as their genotypes in caries-active groups in the Urumqi municipality. CHROMagar Candida was separately cultivated, and we identified 359 Uygur and Han children aged 3-5 years. We randomly selected 20 Han children and 20 Uygur children for this study. We chose a bacterial strain for polymerase chain reaction (PCR) 25S rDNA genotyping and random amplified polymorphic DNA (RAPD) genotyping. The rate of caries-active in Han children was higher than that in Uygur children, with values of 39.6 and 24.3%, respectively. The detection rate of C. albicans was closely correlated to the caries filling index classification (c 2 = 31.037, P = 0.000, r = 0.421; c 2 = 80.454, P = 0.000, r = 0.497). PCR of 25S rDNA from 40 strains of Han and Uygur children revealed 3 genotypes, while RAPD analysis revealed 5 genotypes. The distribution of 25S rDNA genotyping of Han children from PCR differed from that of Uygur children (c 2 = 7.697, P = 0.021), 749©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 748-757 (2015) Candida albicans in children both of which were mainly the A type. RAPD genotyping of both Han and Uygur children showed similar results (c 2 = 1.573, P = 0.814). There were differences in the distributions of C. albicans in children of different nationalities. C. albicans is a key factor causing caries. The PCR 25S rDNA genotyping method is simple and sensitive, while the RAPD genotyping method is reliable and comprehensive.

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Long-term predictive value of salivary microbial diagnostic tests in children

Abstract: Children whose mouths were colonized by salivary lactobacilli at baseline were more susceptible to caries onset in both primary and permanent molars at any given time during follow- up of seven years than children who were not colonized by those caries associated microorganisms.

Cited by 12 publications

References 19 publications

Growth inhibition of oral mutans streptococci and candida by commercial probiotic lactobacilli - an in vitro study

Growth inhibition of oral mutans streptococci and candida by commercial probiotic lactobacilli - an in vitro study

Salivary biomarkers for dental caries

Distribution of Candida albicans in the oral cavity of children aged 3-5 years of Uygur and Han nationality and their genotype in caries-active groups

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