Long term regeneration by somatic embryogenesis in barley (Hordeum vulgare L.) tissue cultures derived from apical meristem explants

Weigel, Russell C.; Hughes, Karen W.

doi:10.1007/bf00040312

Cited by 39 publications

(12 citation statements)

References 21 publications

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“…Our histological sections also showed a differentiation of roots, leafy structures and shoot buds, which is similar to the observations of Weigel and Hughes [29]. Oka et al [18] reported that a barley scutellum-derived callus gave rise to a number of roots without shoots.…”

Section: Discussionsupporting

confidence: 90%

The effect of genotype on a barley scutella culture. Histological aspects

et al. 2013

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Cereals are known to be recalcitrant to the induction of morphogenesis in vitro and the majority of the methods used are callus-mediated and species/genotype-dependent. In the present investigation, a method of morphogenesis induction from immature scutella of selected barley cultivars was used, and particular attention was paid to histology in the initial stages of the regeneration process in order to confirm whether it occurs directly or indirectly (via callus formation). The length of the period from inoculating scutella on the medium to obtaining plantlets depended on the cultivar and the individual scutellum of the barley and varied between 2.5–4 months. The regeneration efficiency and viability of barley scutella was revealed to be highly genotype dependent. The average number of regenerated plants per regenerating scutellum was highest in the case of cv Granal (3.7). A histological analysis of the cultured explants showed both non-morphogenic and morphogenic callus formation. Two types of indirect morphogenetic response were observed: organogenesis (shoot bud formation) and somatic embryogenesis. This is the first report concerning an analysis of in vitro regeneration from immature scutella of barley cultivars (Stratus, Ryton, Granal and Binal).

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Section: Discussionsupporting

confidence: 90%

The effect of genotype on a barley scutella culture. Histological aspects

et al. 2013

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“…In barley, differences in the production of embryogenic calluses and regenerated plants have been observed when cultures were initiated from apical meristems (Weigel & Hughes 1985), immature embryos (Thomas & Scott 1985;Liihrs & Lorz 1987), immature inflorescences (Thomas & Scott 1985), seedling tissues (Rengel & Jelaska 1986), and leaves (Mohanty & Ghosh 1988). Also, differences have been described in the in vitro embryogenic response among different genotypes (Hanzel et al 1985;Goldstein & Kronstad 1986;L/ihrs & Lorz 1987;Picketing 1989).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis, plant regeneration and somaclonal variation in barley

Ruiz

Rueda

Peláez

et al. 1992

Plant Cell Tiss Organ Cult

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In vitro culture of immature embryo and young leaf tissues was carried out with five cultivars of barley, Hordeum vulgare. Two cultivars (Albacete and Porthos) responded poorly from both types of explants, while the three others (Dissa, Golden Promise and Ingrid) produced a high frequency of embryogenic callus from these explants (25-60%). For 'Dissa' and 'Ingrid', young leaf explants were slightly better than immature embryo explants for embryogenic callus induction, while immature embryo cultures of 'Golden Promise' responded better than young leaf explants. Thus, there appears to be a significant genotype × explant interaction in the initiation of embryogenic callus in barley.Some phenotypic variants were detected among the regenerated plants of 'Golden Promise' and 'Ingrid', most originating by epigenetic changes. Only in one case was the variant phenotype heritable, probably due to a mutation in the chloroplast DNA. Mitotic alterations were not detected. Consequently, somaclonal variation did not appear to be a very frequent event in plants regenerated from 1-to 6-month-old cultures of barley.

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“…In barley, as in all of the cereals, a variety of explants have been successfully used for obtaining morphogenesis in vitro (Jähne-Gärtner and Lörz 1996), of which the most common are apical meristems (Cheng and Smith 1975;Weigel and Hughes 1985), mesocotyles (Jelaska et al 1984;Müller et al 1989), seedling segments (Becher et al 1992), mature embryos (Lupotto 1984;Akula et al 1999) and immature inflorescences (Thomas and Scott 1985;Havrlentovµ et al 2001). However, developmental progression has been limited to cultures capable of somatic embryogenesis and plant regeneration directly from the explant or via a callus phase using immature embryos (Lührs and Lörz 1987;Dahleen and Bregitzer 2002) or microspores (Jähne et al 1991;Li and Devaux 2001).…”

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A highly efficient plant regeneration system through multiple shoot differentiation from commercial cultivars of barley (Hordeum vulgare L.) using meristematic shoot segments excised from germinated mature embryos

et al. 2004

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A fast and highly efficient short-term in vitro regeneration system was developed for barley (Hordeum vulgare L.) based on readily available explants. Clumps of multiple shoots and buds suitable for transformation were obtained 9-10 weeks after culture initiation from model and current commercial cultivars. Meristematic shoot segments (MSSs) excised from mature embryo-derived seedlings and subsequently cultured on MS-based medium containing 2 mg/l Picloram and 3 mg/l thidiazuron (TDZ) differentiated up to ten multiple shoots after 3-4 weeks with no or very little callus formation. Sectors of the already multiplied shoot clumps were further multiplied on proliferation-maintenance medium containing 2 mg/l Picloram and 2.5 mg/l TDZ. Biweekly subcultures resulted in a continuous process of multiplication of these highly differentiating green sectors without any loss of morphogenic potential. The differentiated small shoots and shoot buds gave rise to normal shoots on medium with 0.1 mg/l Picloram and 1 mg/l TDZ. After rooting on basal medium with 0.5 mg/l or 1 mg/l IBA the plants were transferred to soil and showed normal growth and fertility compared to the seed-grown plants. All of the genotypes tested formed multiple shoots. The percentage of relative MSS multiplication was 63-83%, and the average number of multiplied shoots per MSS ranged from 16 to 34 among the genotypes after 9-11 weeks.

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Long term regeneration by somatic embryogenesis in barley (Hordeum vulgare L.) tissue cultures derived from apical meristem explants

Cited by 39 publications

References 21 publications

The effect of genotype on a barley scutella culture. Histological aspects

The effect of genotype on a barley scutella culture. Histological aspects

Somatic embryogenesis, plant regeneration and somaclonal variation in barley

A highly efficient plant regeneration system through multiple shoot differentiation from commercial cultivars of barley (Hordeum vulgare L.) using meristematic shoot segments excised from germinated mature embryos

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