Despite the excellent osseointegration of carbon-fiber-reinforced polyetheretherketone (CFR/PEEK) with a surface hydroxyapatite (HA) coating, the bone-implant interfacial shear strength of HA-coated CFR/PEEK after osseointegration is unclear. We examined the interfacial shear strength of HA-coated CFR/PEEK implants after in vivo implantation in a rabbit femur-implant pullout test model. HA coating was performed by a newly developed method. Uncoated CFR/PEEK, HA-coated blasted titanium alloy, and uncoated blasted titanium alloy were used as control implants. The implants were inserted into drilled femoral cortex, and pull-out tests were conducted after 6 and 12 weeks of implantation to determine maximum interfacial shear strength. The HA-coated CFR/ PEEK (15.7 AE 4.5 MPa) and HA-coated titanium alloy (14.1 AE 6.0 MPa) exhibited significantly larger interfacial shear strengths than the uncoated CFR/PEEK (7.7 AE 1.8 MPa) and the uncoated titanium alloy (7.8 AE 2.1 MPa) at 6 weeks. At 12 weeks, only the uncoated CFR/PEEK (8.3 AE 3.0 MPa) exhibited a significantly smaller interfacial shear strength, as compared to the HA-coated CFR/PEEK (17.4 AE 3.6 MPa), HA-coated titanium alloy (14.2 AE 4.8 MPa), and uncoated titanium alloy (15.0 AE 2.6 MPa). Surface analysis of the removed implants revealed detachment of the HA layer in both the HA-coated CFR/PEEK and titanium alloy implants Keywords: carbon-fiber-reinforced PEEK; interfacial shear strength; hydroxyapatite; coating adhesion; osseointegration Carbon-fiber-reinforced polyetheretherketone (CFR/ PEEK) is attractive for use in orthopedic implants, 1-4 due to high fatigue strength, high chemical stability, no metal ion release, and excellent biocompatibility with direct cell attachment. 5,6 Moreover, mechanical properties, including strength, toughness, and stiffness, can be easily modified by the arrangement of reinforcing fiber volume and orientation. In cementless hip prostheses, tailoring stiffness can minimize bone resorption of the proximal femur due to stress shielding by designing CFR/PEEK stems with elasticity similar to surrounding cortical bone. 7-10 The fatigue resistance and toughness could minimize the risk of implant fracture. 11-13 The metal-free nature of CFR/PEEK prostheses eliminate metal allergy concerns 14 and problems passing through security gates with metal detectors. 15 Furthermore, CT and MRI of areas around the implants can be achieved without artifacts. 16 Despite these theoretical advantages, CFR/PEEK cementless hip prostheses are not yet practical for clinical applications. In vivo wear resistance and subsequent immunoreaction have not been established when used as a bearing surface. Another issue is implant fixation. Achieving a stable bone-implant interface by osseointegration has been a longstanding problem with carbon-fiber-reinforced polymer materials, including CFR/PEEK. 17,18 To date, studies have focused on wear reduction 4,19 or secure implant fixation 20-27 to develop cementless CFR/PEEK prostheses.Given that CFR/PEEK is...