Long‐Term Stabilization of the Activity of Ascorbate Oxidase Adsorbed on a Porous Carbon Material by Polymaleimidostyrene

Amperometric Urea Biosensor Using Aminated Glassy Carbon Electrode Covered with Urease Immobilized Carbon Sheet, Based on the Electrode Oxidation of Carbamic Acid

“…PMS contains a large amount of maleimide groups on its polystyrene chain, and sulfhydryl or amino groups of enzymes react with these maleimide groups through covalent bonding. Recently, the use of PMS in enzyme sensor has been proposed to fabricate glucose [23] and L-ascorbate acid [24] electrodes. It is well known that urease contains sulfhydryl groups [25,26] and the activity of glucose oxidase immobilized to PMS depends significantly on the pH value of enzyme immobilization [23].Therefore, the response characteristics of an amperometric urea electrode using PMS have been discussed.…”

Polymers for Biosensors Construction

“…It is popular for the immobilization in enzyme-linked immunosorbent assays (ELIA) by adsorption, however, and few methods for immobilizing proteins on the PS surface by covalent bonding have been proposed, because the complicated multistep methods must be employed for introduction of functional groups that react with proteins and their procedures are tedious and time consuming. In order to solve the difficulty of introduction of functional groups, we adopted polymaleimidostyrene (PMS) to introduce maleimide group in the bulk of PS, and the coating of enzyme containing PS membrane on the electrode surface under mild conditions opens up enormous possibilities for the immobilization of biomolecules [32][33][34][35][36][37][38][39][40]. The interferents and biofouling are two major problems which can affect the performance of a biosensor.…”

Amperometric Glucose Sensor Fabricated by Combining Glucose Oxidase Micelle Membrane and Aminated Glassy Carbon Electrode

“…PMS is a very effective, important, and useful reagent to immobilize enzyme strongly via covalent bond, because high density of maleimide groups of PMS can catch not only exposed SH groups but also buried SH groups (Uchiyama et al 2006;). Moreover, the stability of immobilized enzyme was improved by reacting enzyme to PMS (Tomita et al 2007). PMS exhibits a strong affinity to polystyrene, due to its styrene moiety.…”