Long-term stable hydrogen production from acetate using immobilized Rhodobacter capsulatus in a panel photobioreactor

Elkahlout, Kamal E; Sağır, Emrah; Alipour, Siamak; Koku, Harun; Gündüz, Ufuk; Eroğlu, İnci; Yucel, Meral

doi:10.1016/j.ijhydene.2018.10.133

Cited by 29 publications

(6 citation statements)

References 34 publications

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“…Assessment of the light conversion efficiency (LCE), which is an important parameter to ensure good light utilization and is obtained from the irradiance intensity applied on PBR, will then have to be addressed (Basak et al, 2014; Zhang, Dechatiwongse, et al, 2015). Finally, it should be pointed out that other approaches, such as continuous culture (Basak et al, 2014), biofilm reactors (Fu et al, 2017; Wen et al, 2017), and the use of immobilized cells (Elkahlout et al, 2019; Sagir et al, 2017; Zagrodnik et al, 2015), may stabilize H 2 bioproduction during photofermentation, allowing long‐term operation, although the productivity values are still much lower than those obtained with conventional cultures. These approaches would also allow us to prolong the phase of maximal hydrogen production, which is relatively short when cultures are carried out in batch mode.…”

Section: Resultsmentioning

confidence: 99%

H₂ production by photofermentation in an innovative plate‐type photobioreactor with meandering channels

Turon

Ollivier

Cwicklinski

et al. 2021

Biotech & Bioengineering

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Hydrogen production by Rhodobacter capsulatus is an anaerobic, photobiological process requiring specific mixing conditions. In this study, an innovative design of a photobioreactor is proposed. The design is based on a plate‐type photobioreactor with an interconnected meandering channel to allow culture mixing and H2 degassing. The culture flow was characterized as a quasi‐plug‐flow with radial mixing caused by a turbulent‐like regime achieved at a low Reynolds number. The dissipated volumetric power was decreased 10‐fold while maintaining PBR performances (production and yields) when compared with a magnetically stirred tank reactor. To increase hydrogen production flow rate, several bacterial concentrations were tested by increasing the glutamate concentration using fed‐batch cultures. The maximum hydrogen production flow rate (157.7 ± 9.3 ml H2/L/h) achieved is one of the highest values so far reported for H2 production by R. capsulatus. These first results are encouraging for future scale‐up of the plate‐type reactor.

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Section: Resultsmentioning

confidence: 99%

H₂ production by photofermentation in an innovative plate‐type photobioreactor with meandering channels

Turon

Ollivier

Cwicklinski

et al. 2021

Biotech & Bioengineering

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“…Moreover, they are suitable to prevent culture contamination by unwanted microorganisms. Many PBR designs with symmetrical shapes have been proposed and optimized for the cultivation of PNSB [2,10,[58][59][60]. To date, many studies on PHB production by PNSB have been carried out using small, closed-type PBRs [27,50].…”

Section: Resultsmentioning

confidence: 99%

Poly-β-Hydroxybutyrate Production by Rhodopseudomonas sp. Grown in Semi-Continuous Mode in a 4 L Photobioreactor

et al. 2021

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The synthesis of polyhydroxybutyrate (PHB) by photosynthetic non-sulfur bacteria is a potential approach for producing biodegradable plastics. In this work, acetate was used as a single carbon source to study the effect on PHB formation in Rhodopseudomonas sp. cultured in a cylindrical four-liter photobioreactor under semi-continuous mode. The cultivation process is divided into a symmetrical growth phase and a PHB accumulation phase separated temporally. The symmetrical growth phase (nutrient sufficient conditions) was followed by a sulfur-limited phase to promote PHB accumulation. The main novelty is the progressive lowering of the sulfur concentration into Rhodopseudomonas culture, which was obtained by two concomitant conditions: (1) sulfur consumption during the bacterial growth and (2) semi-continuous growth strategy. This caused a progressive lowering of the sulfur concentration into Rhodopseudomonas culturedue to the sulfur-free medium used to replace 2 L of culture (50% of the total) that was withdrawn from the photobioreactor at each dilution. The PHB content ranged from 9.26% to 15.24% of cell dry weight. At the steady state phase, the average cumulative PHB was >210 mg/L. Sulfur deficiency proved to be one of the most suitable conditions to obtain high cumulative PHB in Rhodopseudomonas culture.

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“…However, their production has been insufficiently explored, being underdeveloped, and remains a promising renewable source of H 2 considering the energy input (sunlight) and purity of product output ( Gupta et al, 2013 ; Chandrasekhar et al, 2015 ; Jiménez-Llanos et al, 2020 ; Chai et al, 2021 ). Several strategies to enhance photo-fermentative biohydrogen production have been described, such as immobilization of bacteria for continuous H 2 production ( Fiβler et al, 1995 ; Elkahlout et al, 2019 ), modification of carbon substrates, nitrogen source, and micronutrients contained in the H 2 production medium ( Liu et al, 2009 ; Laocharoen and Reungsang, 2014 ; Chen et al, 2017 ), and genetic modifications ( Barahona et al, 2016 ; Feng et al, 2018b ; Ma et al, 2021 ), among others.…”

Section: Discussionmentioning

confidence: 99%

A directed genome evolution method to enhance hydrogen production in Rhodobacter capsulatus

Barahona

Isidro²,

Sierra-Heras³

et al. 2022

Front. Microbiol.

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Nitrogenase-dependent H2 production by photosynthetic bacteria, such as Rhodobacter capsulatus, has been extensively investigated. An important limitation to increase H2 production using genetic manipulation is the scarcity of high-throughput screening methods to detect possible overproducing mutants. Previously, we engineered R. capsulatus strains that emitted fluorescence in response to H2 and used them to identify mutations in the nitrogenase Fe protein leading to H2 overproduction. Here, we used ultraviolet light to induce random mutations in the genome of the engineered H2-sensing strain, and fluorescent-activated cell sorting to detect and isolate the H2-overproducing cells from libraries containing 5 × 105 mutants. Three rounds of mutagenesis and strain selection gradually increased H2 production up to 3-fold. The whole genomes of five H2 overproducing strains were sequenced and compared to that of the parental sensor strain to determine the basis for H2 overproduction. No mutations were present in well-characterized functions related to nitrogen fixation, except for the transcriptional activator nifA2. However, several mutations mapped to energy-generating systems and to carbon metabolism-related functions, which could feed reducing power or ATP to nitrogenase. Time-course experiments of nitrogenase depression in batch cultures exposed mismatches between nitrogenase protein levels and their H2 and ethylene production activities that suggested energy limitation. Consistently, cultivating in a chemostat produced up to 19-fold more H2 than the corresponding batch cultures, revealing the potential of selected H2 overproducing strains.

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Long-term stable hydrogen production from acetate using immobilized Rhodobacter capsulatus in a panel photobioreactor

Cited by 29 publications

References 34 publications

H₂ production by photofermentation in an innovative plate‐type photobioreactor with meandering channels

H₂ production by photofermentation in an innovative plate‐type photobioreactor with meandering channels

Poly-β-Hydroxybutyrate Production by Rhodopseudomonas sp. Grown in Semi-Continuous Mode in a 4 L Photobioreactor

A directed genome evolution method to enhance hydrogen production in Rhodobacter capsulatus

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Long-term stable hydrogen production from acetate using immobilized Rhodobacter capsulatus in a panel photobioreactor

Cited by 29 publications

References 34 publications

H2 production by photofermentation in an innovative plate‐type photobioreactor with meandering channels

H2 production by photofermentation in an innovative plate‐type photobioreactor with meandering channels

Poly-β-Hydroxybutyrate Production by Rhodopseudomonas sp. Grown in Semi-Continuous Mode in a 4 L Photobioreactor

A directed genome evolution method to enhance hydrogen production in Rhodobacter capsulatus

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Resources

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H₂ production by photofermentation in an innovative plate‐type photobioreactor with meandering channels

H₂ production by photofermentation in an innovative plate‐type photobioreactor with meandering channels