Long-term storage does not impact the quality of cryopreserved human ovarian tissue

Fabbri, Raffaella; Macciocca, Maria; Vicenti, Rossella; Pasquinelli, Gianandrea; Caprara, Giacomo; Valente, Sabrina; Seracchioli, Renato; Paradisi, Roberto

doi:10.1186/s13048-016-0261-8

Cited by 20 publications

(15 citation statements)

References 40 publications

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“…One report has confirmed normal tissue morphology for up to 18 years following cryopreservation (Fabbri et al, 2016). Ovarian tissue is typically dissected into relatively small pieces that can be more readily cryopreserved relative to large ovarian tissue chunks.…”

Section: Discussionmentioning

confidence: 96%

“…Cryopreserved ovarian tissue from young patients with cancer can contain tens to hundreds of thousands of follicles depending on the age of the patient, and advances in the cryopreservation approach have supported long-term storage of ovarian tissue. One report has confirmed normal tissue morphology for up to 18 years after cryopreservation (Fabbri et al, 2016). Ovarian tissue is typically dissected into relatively small pieces that can be more readily cryopreserved relative to large ovarian tissue chunks.…”

Section: Discussionmentioning

confidence: 98%

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Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Rios

Kniazeva

Lee

et al. 2018

Biotech & Bioengineering

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Cancer survivorship rates have drastically increased due to improved efficacy of oncologic treatments. Consequently, clinical concerns have shifted from solely focusing on survival to quality of life, with fertility preservation as an important consideration. Among fertility preservation strategies for female patients, ovarian tissue cryopreservation and subsequent reimplantation has been the only clinical option available to cancer survivors with cryopreserved tissue. However, follicle atresia after transplantation and risk of reintroducing malignant cells have prevented this procedure from becoming widely adopted in clinics. Herein, we investigated the encapsulation of ovarian follicles in alginate hydrogels that isolate the graft from the host, yet allows for maturation after transplantation at a heterotopic (i.e., subcutaneous) site, a process we termed in vivo follicle maturation. Survival of multiple follicle populations was confirmed via histology, with the notable development of the antral follicles. Collected oocytes (63%) exhibited polar body extrusion and were fertilized by intracytoplasmic sperm injection and standard in vitro fertilization procedures. Successfully fertilized oocytes developed to the pronucleus (14%), two-cell (36%), and four-cell (7%) stages. Furthermore, ovarian follicles cotransplanted with metastatic breast cancer cells within the hydrogels allowed for retrieval of the follicles, and no mice developed tumors after removal of the implant, confirming that the hydrogel prevented seeding of disease within the host. Collectively, these findings demonstrate a viable option for safe use of potentially cancer-laden ovarian donor tissue for in vivo follicle maturation within a retrievable hydrogel and subsequent oocyte collection. Ultimately, this technology may provide novel options to preserve fertility for young female patients with cancer.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 98%

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Rios

Kniazeva

Lee

et al. 2018

Biotech & Bioengineering

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“…Because antral follicles are very large and surrounded by multiple layers of cuboidal granulosa cells, they could not tolerate the freezing damage and maintain their structural integrity during vitri cation [11]. Nevertheless, preantral follicles can maintain their follicular structure and survive the transplantation procedure [10,34]. Vitri cation is a kind of technology that is easy to perform for the simple and quick preservation of ovaries.…”

Section: Discussionmentioning

confidence: 99%

Effect of Resveratrol on Mouse Ovarian Cryopreservation and Transplantation

Wang

Geng

Gan

et al. 2021

Preprint

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BackgroundAfter ovarian tissue transplantation, ischemia-reperfusion injury and free radicals cause follicle depletion and apoptosis. Therefore, the use of antioxidants to reduce the production of free radicals is an important method to address the consequences of ischemia-reperfusion injury. Resveratrol is a natural active polyphenol compound with anti-inflammatory, antitumor, strong antioxidant and anti-free radical properties. The aim of this study was to investigate whether resveratrol could improve the effect of autologous ovarian transplantation after cryopreserve-thawn mouse ovarian tissue.MethodsWhole-ovary vitrification and autotransplantation models were used to investigate the effects of resveratrol. Six-week-old female mice from the Institute of Cancer Research (ICR) were subjected to vitrification. All ovaries were preserved in liquid nitrogen for 1 week before being thawed. After thawing, ovarian tissues were autotransplanted in the bilateral kidney capsules. Mice (n=72) were randomly divided into four groups to determine the optimal concentration of resveratrol (experiment I). Treatments were given as follows: saline, 5 mg/kg resveratrol, 15 mg/kg resveratrol and 45 mg/kg resveratrol, which were administered orally for one week. Grafted ovaries were collected for analysis on days 3, 7, and 21 after transplantation. Ovarian follicle morphology was assessed by hematoxylin and eosin staining. Serum FSH and E2 levels were measured to estimate the transplanted ovarian reserve and endocrine function. Other mice were randomly divided into two groups—saline and 45 mg/kg resveratrol to further evaluate the effect of resveratrol and explore the mechanisms underlying this effect (experiment II). Ovarian follicle apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assays. Immunohistochemistry, qRT-PCR and western blotting (MDA, SOD, NF-κB, IL-6 and SIRT1) were used to explore the mechanisms of resveratrol. Moreover, oocytes derived from autotransplanted ovaries at 21 days were cultured and fertilized in vitro. ResultsThe proportions of morphologically normal (G1) follicles at 3, 7 and 21 days were significantly higher in the 45 mg/kg resveratrol group than in the saline group. The TUNEL-stained follicles (%) at 7 days were significantly decreased in the 45 mg/kg resveratrol group compared with the saline group. Western blot analysis revealed that SOD2 and SIRT1 levels were significantly higher in the 45mg/kg resveratrol group than in the saline group at day 7 and that MDA and NF-κB levels were lower in the saline group on day 3. Likewise, IL-6 was lower in the saline group on day 7. These results are basically consistent with the qRT-PCR results. In addition, the mean number of retrieved oocytes and fertilization and cleavage were significantly increased in the 45 mg/kg resveratrol group compared with the saline group. ConclusionsAdministration of resveratrol could improve the quality of cryopreserved mouse ovarian tissue after transplantation and the embryo outcome, through anti-inflammatory and antioxidative mechanisms.

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“…After patients recover from diseases and when they desire to have children, cryopreserved ovarian tissue can be processed to thaw and transplant as the artificial ovary. Long‐term cryopreservation does not impact the quality of human ovarian tissue and therefore provides flexibility to patients to schedule their pregnancies (Fabbri et al, ). The time for isolating follicles, before or after cryopreservation, has not been found to influence their viability; therefore, follicles can be isolated after thawing cryopreserved ovarian tissue (Vanacker et al, ).…”

Section: Issues Of Preserving Ovarian Tissuementioning

confidence: 99%

A new possibility in fertility preservation: The artificial ovary

Cho

Kim

Noh

et al. 2019

J Tissue Eng Regen Med

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Conventional fertility preservation methods such as oocyte or embryo cryopreservation are currently insufficient to treat including those patients with prepubertal cancer and premature ovarian failure. Ovarian tissue cryopreservation presents as an alternative but has limitations with a potential risk of reintroducing malignant cells in patients who recover from cancer, those of chemotherapy prior to tissue cryopreservation. The so called “artificial ovary” aims to resolve this issue by transplanting isolated follicles with or without a biological scaffold. The artificial ovary may also offer an effective alternative option for those who cannot benefit from traditional assisted reproductive techniques such as in vitro fertilisation. To date, in animal studies and human trial, the artificial ovary restored endocrine function, achieved in vivo follicular development, and resulted in successful pregnancies. However, development of a technique for higher follicular recovery rate and a more optimised design of delivery scaffold, better transplantation techniques to prevent postsurgical ischemia, and consideration for genetic safety are required for safer and consistent human clinical applications. Ideas from different transplantation surgeries (e.g., entire ovary, ovarian cortex, and transplantation with tissue‐engineered products) can be applied to enhance the efficacy of artificial ovarian transplantation. For the better application of artificial ovary, a deeper understanding of mechanical and biochemical properties of the ovary and folliculogenesis after cryopreservation, transplantation with or without scaffold, and development of sophisticated in vivo imaging techniques of transplanted artificial ovary need to precede its efficient clinical application.

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Long-term storage does not impact the quality of cryopreserved human ovarian tissue

Cited by 20 publications

References 40 publications

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Retrievable hydrogels for ovarian follicle transplantation and oocyte collection

Effect of Resveratrol on Mouse Ovarian Cryopreservation and Transplantation

A new possibility in fertility preservation: The artificial ovary

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