Long‐term storage of peripheral blood stem cells frozen and stored with a conventional liquid nitrogen technique compared with cells frozen and stored in a mechanical freezer

McCullough, Jeffrey; Haley, Rebecca; Clay, Mary; Hubel, Allison; Lindgren, Bruce R.; Moroff, Gary

doi:10.1111/j.1537-2995.2009.02482.x

Cited by 75 publications

(59 citation statements)

References 43 publications

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“…Historically, the long-term preservation of mammalian cells at -80°C has met with variable success, with some tumor cell lines and cells destined for transplantation surviving (McCullough et al 2010;Ratajczak et al 1994;, and other cell lines dying within a year (Simione 1992). We report that insect cells can be preserved at -80°C and extend the application of this observation by increasing preserved volumes to levels allowing for regeneration of large scale insect cell expression cultures in a timely manner.…”

Section: Resultsmentioning

confidence: 99%

Long-term, large scale cryopreservation of insect cells at −80 °C

et al. 2014

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Standard tissue culture methods advise freezing cells in small aliquots (B1 9 10 7 cells in 1 mL), and storing in liquid nitrogen. This is inconvenient for laboratories culturing large quantities of insect cells for recombinant baculovirus expression, owing to the length of time taken to produce large scale cultures from small aliquots of cells. Liquid nitrogen storage requires use of specialized cryovials, personal protective equipment and oxygen monitoring systems. This paper describes the long-term, large scale cryopreservation of 8 9 10 8 insect cells at -80°C, using standard 50 mL conical tubes to contain a 40 mL cell suspension. Sf9, Sf21 and High 5 cells were recovered with a viability [ 90 % after storage for one year under these conditions, which compared favorably with the viability of cells stored in liquid nitrogen for the same length of time. Addition of green fluorescent protein encoding baculovirus demonstrated that cells were ''expression ready'' immediately post thaw. Our method enables large scale cultures to be recovered rapidly from stocks cryopreserved at -80°C, thus avoiding the inconvenience, hazards and expense associated with liquid nitrogen.

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Section: Resultsmentioning

confidence: 99%

Long-term, large scale cryopreservation of insect cells at −80 °C

et al. 2014

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“…Albumin, modified gelatin, hydroxyethyl starch(HES), polyvinylpyrrolidone and polyethylene oxide are members of this group of substances. HES has been most widely studied, particularly to reduce the DMSO concentration (Clapisson et al, 2004;Jeffrey McCullough et al, 2010). A combination of 5% DMSO and 6%HES has been shown to be associated with successful long term storage of PBSC (Jeffrey McCullough et al, 2010).…”

Section: Alternatives To Standard Dmsomentioning

confidence: 99%

Cryopreservation of Hematopoietic and Non-Hematopoietic Stem Cells – A Review for the Clinician

Berz¹,

Colvin²

2012

New Advances in Stem Cell Transplantation

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“…In a start-up situation, with limited resources, it is possible to maintain cellular therapy product viability using a ⩽ −70°C mechanical chest freezer instead of a programmable controlled rate freezer and LN 2 storage freezer. [8][9][10] Cellular therapy product stability can be maintained for several months at the warmer temperature by using a cryoprotectant consisting of 6% hydroxyethylstarch and 5% dimethyl sulfoxide (DMSO) and human albumin. 10 For longer-term storage (years) cryopreservation with a final concentration of 10% DMSO and storage in liquid or vapor phase nitrogen is still the preferred and most widely used methodology, as there has been some indication of a fall in GM-CFU (granulocyte macrophage colony-forming unit) colonies after 1 year of storage at − 80°C.…”

Section: Personnelmentioning

confidence: 99%