Long-term survey and characterization of cyflumetofen resistance in Tetranychus urticae populations from Turkey

İnak, Emre; Alpkent, Yasin Nazım; Saalwaechter, Corinna; Albayrak, Tuba; İnak, Arda; Dermauw, Wannes; Geibel, Sven; Leeuwen, Thomas Van

doi:10.1016/j.pestbp.2022.105235

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…H258Y has not yet been reported in recent resistance screens of field populations, 22,42 which might be associated with the high fitness cost. It is likely that the strong impairment of complex II activity by H258Y (and potentially other mutations in complex II) would result in a rapid decrease of allele frequency in the absence of selection pressure, as we have observed in our experimental evolution setup.…”

Section: Discussionmentioning

confidence: 99%

“…[33][34][35][36][37][38] Resistance and cross-resistance cases have already been reported in regions where these products are widely used, with most cases reported in T. urticae populations identifying metabolic resistance as the major resistance mechanism. [39][40][41][42][43] Metabolic resistance to SDH inhibitors in spider mites is mainly mediated by CYP detoxification and leads to low to moderate resistance levels. [44][45][46][47] For example, CYP392A16 was shown to metabolize pyflubumide to a non-toxic metabolite.…”

Section: Introductionmentioning

confidence: 99%

“…Acaricidal SDH inhibitors such as cyflumetofen, cyenopyrafen, pyflubumide and cyetpyrafen belong to the most recently developed mode of action group (IRAC Group 25) [33][34][35][36][37][38] . Resistance and cross-resistance cases have already been reported in regions where these products are widely used, with most cases reported in T. urticae populations identifying metabolic resistance as the major resistance mechanism [39][40][41][42][43] . Metabolic resistance to SDH inhibitors in spider mites is mainly mediated by CYP detoxification and leads to low to moderate resistance levels [44][45][46][47] .…”

Section: Introductionmentioning

confidence: 99%

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The complex II resistance mutation H258Y in succinate dehydrogenase subunit B causes fitness penalties associated with mitochondrial respiratory deficiency

Njiru

Saalwaechter

Mavridis

et al. 2023

Pest Management Science

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BACKGROUNDThe acaricides cyflumetofen, cyenopyrafen and pyflubumide inhibit the mitochondrial electron transport chain at complex II [succinate dehydrogenase (SDH) complex]. A target site mutation H258Y was recently discovered in a resistant strain of the spider mite pest Tetranychus urticae. H258Y causes strong cross‐resistance between cyenopyrafen and pyflubumide, but not cyflumetofen. In fungal pests, fitness costs associated with substitutions at the corresponding H258 position that confer resistance to fungicidal SDH inhibitors have not been uncovered. Here, we used H258 and Y258 near‐isogenic lines of T. urticae to quantify potential pleiotropic fitness effects on mite physiology.RESULTSThe H258Y mutation was not associated with consistent significant changes of single generation life history traits and fertility life table parameters. In contrast, proportional Sanger sequencing and droplet digital polymerase chain reaction showed that the frequency of the resistant Y258 allele decreased when replicated 50:50 Y258:H258 experimentally evolving populations were maintained in an acaricide‐free environment for approximately 12 generations. Using in vitro assays with mitochondrial extracts from resistant (Y258) and susceptible (H258) lines, we identified a significantly reduced SDH activity (48% lower activity) and a slightly enhanced combined complex I and III activity (18% higher activity) in the Y258 lines.CONCLUSIONOur findings suggest that the H258Y mutation is associated with a high fitness cost in the spider mite T. urticae. Importantly, while it is the most common approach, it is clear that only comparing life history traits and life table fecundity does not allow to reliably estimate fitness costs of target site mutations in natural pest populations. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The complex II resistance mutation H258Y in succinate dehydrogenase subunit B causes fitness penalties associated with mitochondrial respiratory deficiency

Njiru

Saalwaechter

Mavridis

et al. 2023

Pest Management Science

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Monitoring acaricide resistance and the frequency of associated target‐site mutations in Tetranychus urticae populations from rose glasshouses in China

Zong,

Song,

Huang

et al. 2024

Pest Management Science

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BACKGROUNDThe two‐spotted spider mite, Tetranychus urticae, is a serious pest of glasshouse roses. The rapid development of resistance to acaricides has severely impacted rose production. To investigate the resistance status of T. urticae in glasshouse rose cultivation in China, we tested the susceptibility of 10 populations to 12 commonly used acaricides and examined the frequency of target‐site mutations associated with acaricide resistance.RESULTSAll of the tested populations showed resistance to at least eight types of acaricides and exhibited high levels of resistance to abamectin, diafenthiuron, hexythiazox and spirodiclofen. The JN‐YN and LZ‐GS populations even developed >70 000‐fold resistance to abamectin. Additionally, resistance to the newly applied bifenazate or cyetpyrafen was detected in eight populations. Many glasshouse‐collected populations showed significantly increased activity of detoxifying enzymes and harbored high frequencies of target‐site mutations known to be associated with resistance. A newly discovered I136A mutation in cytb was detected at >85% frequencies in two populations with high levels of bifenazate resistance, and its frequency showed a strong correlation with the median lethal concentration values in all of the populations.CONCLUSIONSMultiple‐acaricide resistance exists extensively in all collected populations. The high resistance levels of collected populations to many acaricides, together with multiple target‐site mutations, indicate a serious resistance status of T. urticae in rose glasshouses in China. The role of the newly discovered I136A mutation in bifenazate resistance requires further verification. These results will be essential for implementing a well‐thought‐out resistance management program for rose cultivation in China. © 2024 Society of Chemical Industry.

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Acaricidal efficacy of aqueous extracts from different plants on Tetranychus urticae Koch, 1836 (Acari: Tetranychidae)

Alpkent,

Ulusoy,

Ertürk

2024

J Plant Dis Prot

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Long-term survey and characterization of cyflumetofen resistance in Tetranychus urticae populations from Turkey

Cited by 6 publications

References 39 publications

The complex II resistance mutation H258Y in succinate dehydrogenase subunit B causes fitness penalties associated with mitochondrial respiratory deficiency

The complex II resistance mutation H258Y in succinate dehydrogenase subunit B causes fitness penalties associated with mitochondrial respiratory deficiency

Monitoring acaricide resistance and the frequency of associated target‐site mutations in Tetranychus urticae populations from rose glasshouses in China

Acaricidal efficacy of aqueous extracts from different plants on Tetranychus urticae Koch, 1836 (Acari: Tetranychidae)

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