Long‐term survival of human myeloid progenitor cells induced by a mouse bone marrow stromal cell line

Otsuka, Takeshi; Satoh, Hironobu; Ogo, Tomonori; Nakano, Taku; Niho, Yoshiyuki; Bairy, Osnat; Glück, Ursula; Zipori, Dov; Okamura, Seiichi

doi:10.1002/stem.5530100305

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…Primary BM cultures produce stromal layers [5, 7‐9, 11, 12], which after irradiation are able to maintain long‐term culture initiating cells (LTC‐IC) being inoculated as mononuclear cell (MNC) fractions and enriched CD34 + populations from mice [4, 10, 13, 14] and humans [9, 11, 12]. Murine and human cell lines such as M2‐10B4, MS5, and 14F1.1, mostly of embryonal origin, are also able to support growth of hematopoietic cells in vitro [1, 7, 15‐19].…”

Section: Introductionmentioning

confidence: 99%

Mature Accessory Cells Influence Long‐Term Growth of Human Hematopoietic Progenitors on a Murine Stromal Cell Feeder Layer

Mazini¹,

Wunder²,

Sovalat³

et al. 1998

STEM CELLS

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A recently described long-term culture system for early human progenitor cells was established with the murine preadipocyte stromal line FBMD-1 grown in 96-well plates; cobblestone areas formed by inoculated hematopoietic cells are determined in a limiting dilution setting after five weeks' culture. To compare the capacity of cobblestone-area-forming cell (CAFC) formation by bone marrow and leukapheresis products in this system, mononuclear cells (MNC) of both origins were cultured. As related to CD34 + cell content, CAFC yields after five weeks' culture were in the same range in bone marrow and leukapheresis stemming from patients with efficient mobilization of hematopoietic cells. In purified CD34 + cell fractions, the CAFC yield per inoculated cell number was considerably higher than in MNC; however, if the CAFC number was related to the inoculated CD34 + cell number in MNC and after purification, the yield was four to eight times decreased in purified fractions. Addition of the mature cells brought the CAFC yield back up to the numbers obtained in the unseparated MNC fraction. By contrast, slightly more advanced progenitors per CAFC were found in cultures of purified hematopoietic cells from both origins than in whole MNC. The results suggest that mature human accessory cells give noticeable support to recruitment of early progenitors on this feeder but lead to lower yield of GM progenitors.

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Section: Introductionmentioning

confidence: 99%

Mature Accessory Cells Influence Long‐Term Growth of Human Hematopoietic Progenitors on a Murine Stromal Cell Feeder Layer

Mazini¹,

Wunder²,

Sovalat³

et al. 1998

STEM CELLS

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“…The impact of feeder cells on growth conditions has been studied by different groups (5,6,8,10,11,24,25) and has lead to engineering supplementary growth factor genes into murine or human lines (5,11,24). Breems et al (26) have shown that cultures with conditioned medium from FBMD-1 stromal cells, which are known to produce transforming growth factor-b (TGF-b), macrophage colony-stimulating factor (M-CSF), IL-6, and stem cell factor (SCF) (9), supported the growth and expansion of early progenitors from LP better than L87/4 and L88/5 human stromal cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…H UMAN EARLY HEMATOPOIETIC PROGENITORS from bone marrow (BM) and leukapheresis products (LP) are known to develop in long-term culture (LTC) on human allogeneic stroma or on stromal cell lines for up to 3 months (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). Complete stroma provide them with a microenvironment essentially composed of human mesenchymal smooth muscle-like cells, giant fat cells, fibroblasts, osteoblasts, and monocytes/macrophages (1,2,12); optimal growth and maintenance of early progenitors within the stroma as cobblestone areas (CAFC) are observed in the presence of these varied cell types (1)(2)(3)(4)(12)(13)(14), and obviates addition of exogenous growth factors, which are essential for LTC in human and most murine stromal cell lines (11).…”

Section: Introductionmentioning

confidence: 99%

Human Accessory Cells Have a Humoral Bystander Effect on CAFC Growing on Murine Feeder

Mazini

Wunder²,

Sovalat³

et al. 2000

Journal of Hematotherapy & Stem Cell Research

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Human early hematopoietic progenitors from bone marrow (BM) and leukapheresis products (LP) are highly proliferative in presence of accessory cells in standard culture on the murine FBMD-1 cell feeder with weekly addition of human interleukin-3 (HuIL-3) and granulocyte-colony stimulating factor (HuG-CSF). If however purified CD34+ cells are cultured under otherwise identical conditions, cobblestone areas (CAFC) formed by the same number of target cells are diminished by more than 1 log, as we showed previously. This suggests that mature cells are involved in growth of early progenitors. To determine whether this bystander effect is mediated by soluble growth factors, or by direct cell-to-cell contact with early progenitors, we stimulated mature plastic adherent cells separately and tested the resulting conditioned supernatant (ACS) on CAFC and colony-forming unit-granulocyte-macrophage (CFU-GM) production. In ACS-complemented standard cultures of purified CD34+ cells, the yield of CAFC was up to 1 log higher if compared to parallel cultures without ACS. Likewise, the CFU-GM production was enhanced in presence of ACS, especially in the adherent fraction of the culture. When CD34+ cell cultures were performed with ACS but without added interleukin-3 (IL-3) and granulocyte colony-stimulating factor (G-CSF), CAFC production was in the same range as if these growth factors were added alone. Addition of anti-G-CSF antibody (Ab) to ACS decreased CAFC recruitment significantly, whereas anti-IL-3 Ab had no significant effect. These findings suggest that ACS complemented with IL-3 and G-CSF replaces the accessory cells largely; this is not only due to presence of G-CSF, because ACS in combination with recombinant growth factors mounts CAFC yield higher than saturating amounts of growth factors alone do. There must be further synergizing soluble factors in the supernatant.

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Stem Cell Niches

Zipori

2009

Biology of Stem Cells and the Molecular Basis of the Stem State

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Long‐term survival of human myeloid progenitor cells induced by a mouse bone marrow stromal cell line

Cited by 6 publications

References 18 publications

Mature Accessory Cells Influence Long‐Term Growth of Human Hematopoietic Progenitors on a Murine Stromal Cell Feeder Layer

Mature Accessory Cells Influence Long‐Term Growth of Human Hematopoietic Progenitors on a Murine Stromal Cell Feeder Layer

Human Accessory Cells Have a Humoral Bystander Effect on CAFC Growing on Murine Feeder

Stem Cell Niches

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