Long-term, Sustained Feeding by Asian citrus psyllid Disrupts Salicylic Acid Homeostasis in Sweet Orange

Abstract

Background Phloem-feeding insects are known to modulate the salicylic acid (SA) signaling pathway in various plant-insect interaction models. Diaphorina citri is a phloem feeding vector of a deadly phytopathogen and interactions with its host that may modulate plant defense are not well understood. The objectives of this study were to investigate the molecular mechanisms involved in transcriptional regulation of SA modification and activation of defense-associated responses in sweet orange ( Citrus sinensis ) exposed to various durations (7-, 14- and 150- days) of continuous feeding by D. citri .. Results We quantified expression of genes involved in SA pathway activation and subsequent modification, as well as, associated SA metabolites (SA methyl ester, 2,3-Dihydroxybenzoic acid and SA 2-O-β-D-glucoside). NPR1 and PR-1 expression was upregulated in plants exposed to continuous feeding by D. citri for 14 days. Expression of BSMT-like , MES1-like and DMR6-like oxygenase , as well as, accumulation of their respective SA metabolites (SA methyl ester, 2,3-Dihydroxybenzoic acid) was significantly higher in plants exposed to continuous feeding by D. citri for 150 days than in those without D. citri infestation. Concomitantly, expression of UGT74F2-like was significantly downregulated and its metabolite, salicylic acid 2-β-D-glucoside, was highly accumulated in trees exposed to 150 d of feeding compared to control trees without D. citri . Conclusions D. citri herbivory differentially regulated transcription and SA-metabolite accumulation in citrus leaves, depending on duration of insect feeding. Our results suggest that prolonged and uninterrupted exposure (150 d) of citrus to D. citri feeding suppressed plant immunity and inhibited growth, which may highlight the importance of vector suppression as part of HLB management in citrus.