Long-term transcriptional memory in rice plants submitted to salt shock

Amaral, Marcelo Nogueira do; Arge, Luis Willian Pacheco; Auler, Priscila Ariane; Rossatto, Tatiana; Milech, Cristini; Magalhaes, A. M. de; Braga, Eugênia Jacira Bolacel

doi:10.1007/s00425-020-03397-z

Cited by 13 publications

(10 citation statements)

References 75 publications

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“…A similar relation was noted for the transcriptomic analysis of sensitive and tolerant plants, where the highest number of regulated transcripts was noted for sensitive cucumber after recurring hypoxia [25]. Data concerning the response of priming versus non-priming plants revealed the increasing number of differentially expressed genes for salinity priming rice [36] and cold priming Arabidopsis [37]. Generally, a higher reaction level of response (changes in hormones, metabolites, and other signals) in primed plants was reported [38].…”

Section: Global Proteome Analysis After 2xh Candidates For Priming An...supporting

confidence: 69%

Proteomic Studies of Roots in Hypoxia-Sensitive and -Tolerant Tomato Accessions Reveal Candidate Proteins Associated with Stress Priming

Czernicka

Kęska

Planchon

et al. 2022

Cells

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Tomato (Solanum lycopersicum L.) is a vegetable frequently exposed to hypoxia stress induced either by being submerged, flooded or provided with limited oxygen in hydroponic cultivation systems. The purpose of the study was to establish the metabolic mechanisms responsible for overcoming hypoxia in two tomato accessions with different tolerance to this stress, selected based on morphological and physiological parameters. For this purpose, 3-week-old plants (plants at the juvenile stage) of waterlogging-tolerant (WL-T), i.e., POL 7/15, and waterlogging-sensitive (WL-S), i.e., PZ 215, accessions were exposed to hypoxia stress (waterlogging) for 7 days, then the plants were allowed to recover for 14 days, after which another 7 days of hypoxia treatment was applied. Root samples were collected at the end of each time-point and 2D-DIGE with MALDI TOF/TOF, and expression analyses of gene and protein-encoded alcohol dehydrogenase (ADH2) and immunolabelling of ADH were conducted. After collating the obtained results, the different responses to hypoxia stress in the selected tomato accessions were observed. Both the WL-S and WL-T tomato accessions revealed a high amount of ADH2, which indicates an intensive alcohol fermentation pathway during the first exposure to hypoxia. In comparison to the tolerant one, the expression of the adh2 gene was about two times higher for the sensitive tomato. Immunohistochemical analysis confirmed the presence of ADH in the parenchyma cells of the cortex and vascular tissue. During the second hypoxia stress, the sensitive accession showed a decreased accumulation of ADH protein and similar expression of the adh2 gene in comparison to the tolerant accession. Additionally, the proteome showed a greater protein abundance of glyceraldehyde-3-phosphate dehydrogenase in primed WL-S tomato. This could suggest that the sensitive tomato overcomes the oxygen limitation and adapts by reducing alcohol fermentation, which is toxic to plants because of the production of ethanol, and by enhancing glycolysis. Proteins detected in abundance in the sensitive accession are proposed as crucial factors for hypoxia stress priming and their function in hypoxia tolerance is discussed.

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Section: Global Proteome Analysis After 2xh Candidates For Priming An...supporting

confidence: 69%

Proteomic Studies of Roots in Hypoxia-Sensitive and -Tolerant Tomato Accessions Reveal Candidate Proteins Associated with Stress Priming

Czernicka

Kęska

Planchon

et al. 2022

Cells

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“…This may indicate that first waterlogging treatment enhanced tolerance to recurring waterlogging stress in the sensitive accession. An increased number of DEGs in primed plants, compared to non-primed plants, was also observed in rice, where salt shock was used as the priming factor [64] and in radish, where cold priming was applied [65]. To the best of our knowledge, there is no information on the differential number of regulated genes between plants treated once with hypoxic stress and plants treated again with stress, thus these results are novel and unique in the area of waterlogging tolerance in cucumber.…”

Section: Changes At the Transcriptomic Levelmentioning

confidence: 73%

Long-Term Waterlogging as Factor Contributing to Hypoxia Stress Tolerance Enhancement in Cucumber: Comparative Transcriptome Analysis of Waterlogging Sensitive and Tolerant Accessions

Kęska

Szcześniak

Makałowska

et al. 2021

Genes

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Waterlogging (WL), excess water in the soil, is a phenomenon often occurring during plant cultivation causing low oxygen levels (hypoxia) in the soil. The aim of this study was to identify candidate genes involved in long-term waterlogging tolerance in cucumber using RNA sequencing. Here, we also determined how waterlogging pre-treatment (priming) influenced long-term memory in WL tolerant (WL-T) and WL sensitive (WL-S) i.e., DH2 and DH4 accessions, respectively. This work uncovered various differentially expressed genes (DEGs) activated in the long-term recovery in both accessions. De novo assembly generated 36,712 transcripts with an average length of 2236 bp. The results revealed that long-term waterlogging had divergent impacts on gene expression in WL-T DH2 and WL-S DH4 cucumber accessions: after 7 days of waterlogging, more DEGs in comparison to control conditions were identified in WL-S DH4 (8927) than in WL-T DH2 (5957). Additionally, 11,619 and 5007 DEGs were identified after a second waterlogging treatment in the WL-S and WL-T accessions, respectively. We identified genes associated with WL in cucumber that were especially related to enhanced glycolysis, adventitious roots development, and amino acid metabolism. qRT-PCR assay for hypoxia marker genes i.e., alcohol dehydrogenase (adh), 1-aminocyclopropane-1-carboxylate oxidase (aco) and long chain acyl-CoA synthetase 6 (lacs6) confirmed differences in response to waterlogging stress between sensitive and tolerant cucumbers and effectiveness of priming to enhance stress tolerance.

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“…Transcriptomics has also facilitated the identification of primable genes in response to other abiotic stresses, including salt ( Hu et al, 2016 ; Liu et al, 2019a ; do Amaral et al, 2020a ), heat ( Liu et al, 2018 ), cold ( Zuther et al, 2019 ; Mayer and Charron, 2021 ), excess light ( Crisp et al, 2017 ), and mechanical stress ( Pomiès et al, 2017 ). Much information is now available for assessing PSM maintenance using the transcript abundances of primable genes as molecular outputs.…”

Section: Assessment Of Memory During Abiotic Stress Responsesmentioning

confidence: 99%

Maintenance of abiotic stress memory in plants: Lessons learned from heat acclimation

Charng

Mitra

2022

The Plant Cell

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Plants acquire enhanced tolerance to intermittent abiotic stress by employing information obtained during prior exposure to an environmental disturbance, a process known as acclimation or defense priming. The capacity for stress memory is a critical feature in this process. The number of reports related to plant stress memory (PSM) has recently increased, but few studies have focused on the mechanisms that maintain PSM. Identifying the components involved in maintaining PSM is difficult due in part to the lack of clear criteria to recognize these components. In this review, based on what has been learned from genetic studies on heat acclimation memory, we propose criteria for identifying components of the regulatory networks that maintain PSM. We provide examples of the regulatory circuits formed by effectors and regulators of PSM. We also highlight strategies for assessing PSMs, update the progress in understanding the mechanisms of PSM maintenance, and provide perspectives for the further development of this exciting research field.

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Long-term transcriptional memory in rice plants submitted to salt shock

Cited by 13 publications

References 75 publications

Proteomic Studies of Roots in Hypoxia-Sensitive and -Tolerant Tomato Accessions Reveal Candidate Proteins Associated with Stress Priming

Proteomic Studies of Roots in Hypoxia-Sensitive and -Tolerant Tomato Accessions Reveal Candidate Proteins Associated with Stress Priming

Long-Term Waterlogging as Factor Contributing to Hypoxia Stress Tolerance Enhancement in Cucumber: Comparative Transcriptome Analysis of Waterlogging Sensitive and Tolerant Accessions

Maintenance of abiotic stress memory in plants: Lessons learned from heat acclimation

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