Analysis of a 39-yr record of temperature profiles from Lake Kinneret, Israel, revealed that average epilimnion thickness decreased by , 1.2 m (, 3 cm yr 21 ), metalimnion thickness decreased by between , 1.0 m (December) and , 2.0 m (April), and average temperature of the epilimnion increased by , 1uC (, 0.028uC yr 21 ). Average hypolimnetic temperature remained constant (, 15uC), so that the thermal gradient across the metalimnion increased. The average duration of the stratification period (, 286 d) remained unchanged. We show that the stratification changes were the result of a decrease in lake level, a slight increase in air temperature during the spring and summer, and a reduction of inflows to the lake. These changes in time affected the stratification pattern in the same direction, i.e., an increase in the lake epilimnetic temperature and an increase in the thermal gradient across the metalimnion. Analysis of the data indicates that the effect of overpumping, which leads to a reduction in lake level, is larger than the effect of changes due to air-temperature increase and the reduction in inflow volumes.Many subtropical and temperate lakes are thermally stratified over the course of the year, resulting in a wellmixed warm surface layer (epilimnion), a transition layer (metalimnion) with a steep temperature gradient, and colder and relatively isothermal bottom waters (hypolimnion). The onset, strength, and duration of vertical stratification depend on several internal lake-specific factors and the external influence of weather. Water depth, water transparency, lake size, and the length of fetch along the direction of the prevailing wind are all lake-specific factors that influence the characteristics and strength of vertical stratification. Similarly, radiation, air temperature, wind speeds, and wind shadowing by hills or mountains surrounding the lake are weather-driven factors affecting lake stratification.In thermally stratified eutrophic lakes, there is a hydrochemical differentiation of the water column into an oxic epilimnion and an anoxic hypolimnion caused by the interplay between physical forcing and a succession of microbiological processes (Wetzel 1983). With the onset of thermal stratification, hypolimnetic dissolved oxygen is gradually depleted, while the epilimnion often becomes nutrient depleted (Nishri et al. 2000;Eckert et al. 2002). The result is a water column that consists of a gradual vertical gradient in chemical and biological characteristics, which has an effect on the replenishment of nutrients and on the biological activity in the lake and is greatly affected by vertical mixing processes (Adrian et al. 2009).Long-term changes in the thermal stratification are therefore expected to be the trigger for considerable changes in the biology of a lake. These stratification changes can be caused by (1) Lake Kinneret, in northern Israel (Fig. 1a), is an example of a thermally stratified lake that has exhibited stratification changes over the recent past (Hambright et al. 1994). ...