Long‐term warming effects on the microbiome and<i>nifH</i>gene abundance of a common moss species in sub‐Arctic tundra

Klarenberg, Ingeborg J.; Keuschnig, Christoph; Colmenares, Ana J. Russi; Warshan, Denis; Jungblut, Anne D.; Jónsdóttir, Ingibjörg S.; Vilhelmsson, Oddur

doi:10.1111/nph.17837

Cited by 30 publications

(22 citation statements)

References 87 publications

(111 reference statements)

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“…In contrast, moderate warming of 1.4°C for 20 years led to a decrease in Nostoc relative abundance that was associated with the moss Racomitrium sp. in Iceland (Klarenberg et al, 2021). The effect of temperature elevation on moss‐associated cyanobacterial relative abundance may thus depend on the severity and duration of warming, as well as the location and moss species being considered.…”

Section: Discussionmentioning

confidence: 99%

New insights into the drivers of moss‐associated nitrogen fixation and cyanobacterial biomass in the eastern Canadian boreal forest

et al. 2022

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Moss‐associated cyanobacteria nitrogen (N2‐) fixation can contribute to support moss growth and constitutes a major source of new N in boreal forest ecosystems. The biomass of moss‐colonizing cyanobacteria and their N2‐fixation are usually considered linearly correlated. Yet, recent evidence has shown that cyanobacterial biomass and N2‐fixation can be decoupled, suggesting that they are not necessary affected by the same environmental and ecological drivers. Climate and nutrients were reported as affecting moss‐associated N2‐fixation, with equivocal results, whereas drivers of moss cyanobacterial biomass remain unclear. In addition, these drivers are often determined through manipulative experiments (e.g. fertilization and incubation) and remain to be validated with complementary observational studies to help us better understand future impacts of global change on the moss–cyanobacteria symbiosis. We hypothesized that moss‐associated cyanobacterial biomass is controlled in situ by factors affecting bacterial growth, whereas N2‐fixation is controlled by factors affecting enzymatic reactions. Using random forests, Spearman correlations and linear mixed‐effects models, we determined the main drivers of cyanobacterial biomass and N2‐fixation of two feather moss species, which were collected over 3 years along a 1000‐km latitudinal transect in the eastern Canadian boreal forest. We found that temperature, precipitation and phosphorus were the main positive drivers of moss cyanobacterial biomass and that temperature and molybdenum were the main positive drivers of N2‐fixation. Vanadium was a negative driver of N2‐fixation, suggesting the use of alternative nitrogenases by cyanobacteria. Both cyanobacterial biomass and N2‐fixation were strongly influenced by the moss species and were negatively correlated with moss C:N stoichiometry, highlighting the role of N2‐fixation in moss N enrichment. Synthesis. We identified for the first time some environmental drivers of moss‐associated cyanobacterial biomass and showed that they contrast with the drivers of N2‐fixation, which should be considered in further research and confirmed in other experimental settings. This is an important advance in our knowledge of moss–cyanobacteria associations, which would greatly help in better predicting the impacts of global change on this symbiosis and on nitrogen inputs in boreal forest ecosystems.

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Section: Discussionmentioning

confidence: 99%

New insights into the drivers of moss‐associated nitrogen fixation and cyanobacterial biomass in the eastern Canadian boreal forest

et al. 2022

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“…Instead, the less abundant Stigonema cluster contributed > 80% to total moss N 2 fixation (Warshan et al ., 2016). However, Nostoc is the genus that may become the dominant one associating with mosses in a future, warmer climate (Carrell et al ., 2019; Klarenberg et al ., 2022), with consequences for ecosystem N input that channels through the moss–cyanobacteria pathway.…”

Section: Interactions Between Biotic and Abiotic Controlsmentioning

confidence: 99%

Biotic and abiotic controls of nitrogen fixation in cyanobacteria–moss associations

Rousk

2022

New Phytologist

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Summary Most mosses are colonized by nitrogen (N)‐fixing cyanobacteria. This discovery is relatively recent, which can explain the large knowledge gaps the field is now tackling. For instance, while we have a good understanding of the abiotic controls (e.g. nutrient availability, increased temperature), we still do not know much about the biotic controls of N2 fixation in mosses. I propose here that we should endeavour to position moss–cyanobacteria associations along the mutualism–parasitism continuum under varying abiotic conditions (e.g. nutrient availability). This would finally unravel the nature of the relationship between the partners and will be a big leap in our understanding of the evolution of plant–bacteria interactions using moss–cyanobacteria associations as a model system.

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“…The bacterial communities of Racomitrium moss species and underlying soil were clearly distinct at the ASV level, indicating that there might be little or no lateral transmission of the soil bacterial communities to the moss bacterial communities and/or vice versa. The bacterial community of R. lanuginosum in the Fláajökull glacier forefield was also similar to the bacterial community of R. lanuginosum from a subarctic-alpine heathland in northwest Iceland (Klarenberg et al 2021). Many taxa are shared in similar proportions, such as the orders Acetobacterales, Acidobacterales and Solibacterales, while Bacteroidetes were more abundant in the mosses in the glacier forefield and Planctomycetes more abundant in the heathland.…”

Section: Discussionmentioning

confidence: 61%

Moss functional traits are important drivers for moss and underlying soil bacterial communities: evidence from a chronosequence in an Icelandic glacier forefield

Klarenberg

Keuschnig

Salazar

et al. 2022

Preprint

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Mosses are among the first colonizing organisms after glacier retreat and can develop into thick moss mats during later successional stages. They are key players in N2 fixation through their microbiome, which is an important process for nutrient build-up during primary succession. How these moss-microbe interactions develop during succession is not well-studied and is relevant in the light of climate change and increased glacier retreat. We examined how the bacterial communities associated with two moss species of the genus Racomitrium and the underlying substrate, as well as moss traits and nitrogen fixation, develop along a successional gradient in the glacier forefield of Fláajökull in southeast Iceland. In addition, tested whether moss functional traits, such as total carbon (TC) and nitrogen contents (TN) are drivers of moss and underlying soil bacterial communities. Although time since deglaciation did not affect TN and moisture content, TC and shoot length increased with time since deglaciation. Moss and underlying soil bacterial communities were distinct. While the soil bacterial community structure was driven by the time since deglaciation and moss C/N ratios, the moss bacterial community structure was linked to time since deglaciation and moss moisture content. Moss N2-fixation rates were linked to bacterial community composition and nifH gene abundance rather than moss TN or time since deglaciation. This was accompanied by a shift from autotrophic to heterotrophic diazotrophs. Overall, our results suggest that there is little lateral transfer between moss and soil bacterial communities and that moss traits and time since deglaciation affect moss and soil bacterial community structure. In addition, moss N2-fixation rates are determined by bacterial community structure, rather than moss traits or time since deglaciation.

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Long‐term warming effects on the microbiome andnifHgene abundance of a common moss species in sub‐Arctic tundra

Cited by 30 publications

References 87 publications

New insights into the drivers of moss‐associated nitrogen fixation and cyanobacterial biomass in the eastern Canadian boreal forest

New insights into the drivers of moss‐associated nitrogen fixation and cyanobacterial biomass in the eastern Canadian boreal forest

Biotic and abiotic controls of nitrogen fixation in cyanobacteria–moss associations

Moss functional traits are important drivers for moss and underlying soil bacterial communities: evidence from a chronosequence in an Icelandic glacier forefield

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